Regulation of yeast DNA polymerase δ-mediated strand displacement synthesis by 5\u27-flaps [PDF]
, 2015 The strand displacement activity of DNA polymerase δ is strongly stimulated by its interaction with proliferating cell nuclear antigen (PCNA). However, inactivation of the 3′–5′ exonuclease activity is sufficient to allow the polymerase to carry out ...
Burgers, Peter M +3 more
core +2 more sources
Strand displacement synthesis by yeast DNA polymerase ε [PDF]
Nucleic Acids Research, 2016 DNA polymerase ε (Pol ε) is a replicative DNA polymerase with an associated 3'-5' exonuclease activity. Here, we explored the capacity of Pol ε to perform strand displacement synthesis, a process that influences many DNA transactions in vivo We found that Pol ε is unable to carry out extended strand displacement synthesis unless its 3'-5' exonuclease ...
Ganai, Rais A +3 more
openaire +5 more sources
A Novel Computational Method to Reduce Leaky Reaction in DNA Strand Displacement
Journal of Analytical Methods in Chemistry, 2015 DNA strand displacement technique is widely used in DNA programming, DNA biosensors, and gene analysis. In DNA strand displacement, leaky reactions can cause DNA signals decay and detecting DNA signals fails.
Xin Li +5 more
doaj +1 more source
Single-molecule dilution and multiple displacement amplification for molecular haplotyping
BioTechniques, 2005 Separate haploid analysis is frequently required for heterozygous genotyping to resolve phase ambiguity or confirm allelic sequence. We demonstrate a technique of single-molecule dilution followed by multiple strand displacement amplification to ...
Philip Paul, Josh Apgar
doaj +1 more source
DNA dynamics and computation based on toehold-free strand displacement
Nature Communications, 2021 Synthetic DNA constructs can to used to recognise and respond to input signals. Here the authors present complex DNA nanostructures with toehold-free strand displacement for generation of ON/OFF switches and Boolean gates.
Hong Kang +5 more
doaj +1 more source
DNA multi-bit non-volatile memory and bit-shifting operations using addressable electrode arrays and electric field-induced hybridization. [PDF]
, 2018 DNA has been employed to either store digital information or to perform parallel molecular computing. Relatively unexplored is the ability to combine DNA-based memory and logical operations in a single platform.
Heller, Michael J +3 more
core +3 more sources
Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA [PDF]
Scientific Reports, 2015 Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement.
Khodakov, D. +4 more
openaire +3 more sources
Availability: A Metric for Nucleic Acid Strand Displacement Systems [PDF]
, 2016 DNA strand displacement systems have transformative potential in synthetic biology. While powerful examples have been reported in DNA nanotechnology, such systems are plagued by leakage, which limits network stability, sensitivity, and scalability.
Goltry, Sara +8 more
core +3 more sources
BioTechniques, 2014 The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov +5 more
doaj +1 more source
Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]
, 2013 The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M +4 more
core +1 more source