Results 41 to 50 of about 37,201 (270)

Nucleic acid strand displacement – from DNA nanotechnology to translational regulation

RNA Biology, 2023
Nucleic acid strand displacement reactions involve the competition of two or more DNA or RNA strands of similar sequence for binding to a complementary strand, and facilitate the isothermal replacement of an incumbent strand by an invader.
Friedrich C. Simmel
doaj   +1 more source

Mechanism of strand displacement DNA synthesis by the coordinated activities of human mitochondrial DNA polymerase and SSB

, 2023
Many replicative DNA polymerases couple DNA replication and unwinding activities to perform strand displacement DNA synthesis, a critical ability for DNA metabolism.
Plaza-G A, Ismael, Plaza-G.A., Ismael, Ibarra, Borja, Cao García, Francisco Javier, Plaza-G A., Ismael, Lemishko, Kateryna M., Truong, Thinh Q., Crespo, Rodrigo, Ciesielski, Grzegorz L, Cao-García, Francisco J, Kaguni, Laurie S, Kaguni, Laurie S., Cao-García, Francisco J., Truong, Thinh Q, Ciesielski, Grzegorz L., Lemishko, Kateryna M   +15 more
core   +1 more source

Single-molecule dilution and multiple displacement amplification for molecular haplotyping

BioTechniques, 2005
Separate haploid analysis is frequently required for heterozygous genotyping to resolve phase ambiguity or confirm allelic sequence. We demonstrate a technique of single-molecule dilution followed by multiple strand displacement amplification to ...
Philip Paul, Josh Apgar
doaj   +1 more source

Strong sequence dependence in RNA/DNA hybrid strand displacement kinetics

, 2023
Strand displacement reactions underlie dynamic nucleic acid nanotechnology. The kinetic and thermodynamic features of DNA-based displacement reactions are well understood and well predicted by current computational models.
Smith, Francesca G, Stevens, Molly M, Goertz, John P, Ouldridge, Thomas E   +3 more
core   +1 more source

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA [PDF]

Scientific Reports, 2015
Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement.
Khodakov, D., Khodakova, A., Huang, D., Linacre, A., Ellis, A.   +4 more
openaire   +3 more sources

Control of DNA Strand Displacement Kinetics Using Toehold Exchange [PDF]

, 2009
DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions.
Winfree, Erik, Erik Winfree, David Yu Zhang, Zhang, David Yu   +3 more
core   +1 more source

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

A Double-Layer Image Encryption Scheme Based on Chaotic Maps and DNA Strand Displacement

Journal of Chemistry, 2022
The image encryption schemes combining chaotic maps, DNA coding, and DNA sequence operation can effectively protect the image. In this paper, a double-layer image encryption scheme is proposed by combining chaotic maps with DNA strand displacement (DSD).
Zhen Tang, Zhixiang Yin, Risheng Wang, Xiyuan Wang, Jing Yang, Jianzhong Cui   +5 more
doaj   +1 more source

Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

Electrochemistry Communications, 2023
In this work, a novel strand displacement polymerization strategy is designed with a single hairpin-structured DNA probe acting as both of primer and template.
Yulin Zhu, Xifeng Chen, Jiayue Shi, Haixuan Sun, Hua Chai, Peng Miao   +5 more
doaj   +1 more source

Design of DNA strand displacement reactions

Current Opinion in Biotechnology
DNA strand displacement (SD) reactions are central to the operation of many synthetic nucleic acid systems, including molecular circuits, sensors, and machines. Over the years, a broad set of design frameworks has emerged to accommodate various functional goals, initial configurations, and environmental conditions. Nevertheless, key challenges persist,
Križan Jurinović, Merry Mitra, Rakesh Mukherjee, Thomas E Ouldridge   +3 more
openaire   +3 more sources