Results 41 to 50 of about 78,386 (303)

Sequencing double-stranded DNA by strand displacement [PDF]

Nucleic Acids Research, 1997
Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides.
D J, Fu, H, Köster, C L, Smith, C R, Cantor   +3 more
openaire   +2 more sources

Scalable DNA recognition circuits based on DNA strand displacement. [PDF]

Nanoscale Adv
A scalable recognition circuit was designed and implemented using DNA and eventually a molecular comparator was constructed.
Wang F, Shi B, Chen Y, Shi X, Kou Z, Qiang X.   +5 more
europepmc   +3 more sources

Rational Design of pH-Controlled DNA Strand Displacement [PDF]

Journal of the American Chemical Society, 2014
Achieving strategies to finely regulate with biological inputs the formation and functionality of DNA-based nanoarchitectures and nanomachines is essential toward a full realization of the potential of DNA nanotechnology. Here we demonstrate an unprecedented, rational approach to achieve control, through a simple change of the solution's pH, over an ...
Alessia Amodio, Bin Zhao, Alessandro Porchetta, Andrea Idili, Matteo Castronovo, Chunhai Fan, Francesco Ricci   +6 more
openalex   +6 more sources

Rapid Visual Authentication Based on DNA Strand Displacement [PDF]

ACS Applied Materials & Interfaces, 2021
Novel ways to track and verify items of a high value or security is an ever-present need. Taggants made from deoxyribonucleic acid (DNA) have several advantageous properties, such as high information density and robust synthesis; however, existing methods require laboratory techniques to verify, limiting applications.
Kimberly L. Berk, Steven M. Blum, Vanessa L. Funk, Yuhua Sun, In-Young Yang, Mark V. Gostomski, Pierce A. Roth, Alvin T. Liem, Peter A. Emanuel, Michael E. Hogan, Aleksandr E. Miklos, Matthew W. Lux   +11 more
openaire   +2 more sources

Control of DNA Strand Displacement Kinetics Using Toehold Exchange [PDF]

, 2009
DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions.
Winfree, Erik, Zhang, David Yu
core   +2 more sources

The Effect of Basepair Mismatch on DNA Strand Displacement. [PDF]

Biophys J, 2016
DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized.
Broadwater DWB, Kim HD.
europepmc   +5 more sources

Leakless DNA Strand Displacement Systems [PDF]

, 2015
While current experimental demonstrations have been limited to small computational tasks, DNA strand displacement systems DSD systemsi¾?[25] hold promise for sophisticated information processing within chemical or biological environments. A DSD system encodes designed reactions that are facilitated by three-way or four-way toehold-mediated strand ...
Chris Thachuk, Erik Winfree, David Soloveichik   +2 more
openaire   +2 more sources

Post-replicative nick translation occurs on the lagging strand during prolonged depletion of DNA ligase I in Saccharomyces cerevisiae

G3: Genes, Genomes, Genetics, 2021
During lagging-strand synthesis, strand-displacement synthesis by DNA polymerase delta (Pol ∂), coupled to nucleolytic cleavage of DNA flap structures, produces a nick-translation reaction that replaces the DNA at the 5′ end of the preceding Okazaki ...
Natasha C Koussa, Duncan J Smith
doaj   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

Investigating the dynamics of surface-immobilized DNA nanomachines [PDF]

, 2016
Surface-immobilization of molecules can have a profound influence on their structure, function and dynamics. Toehold-mediated strand displacement is often used in solution to drive synthetic nanomachines made from DNA, but the effects of surface ...
AW Peterson, B Wei, B Yurke, BD Vogt, BJ Thompson, BM Frezza, D Han, D Wang, D Yao, DY Zhang, DY Zhang, DY Zhang, F Dannenberg, F Ricci, G Papadakis, G Papadakis, G Sauerbrey, G Seelig, H Chandran, H Dietz, HM Watkins, J SantaLucia, JC Love, JH Chen, JN Zadeh, K Lund, KE Dunn, L Qian, L Qian, L Qian, LM Adelman, LM Smith, M Castronovo, M Rodahl, M Teichmann, MC Dixon, N Srinivas, P Xu, PWK Rothemund, Q Liu, RRF Machinek, S Woo, SB Smith, SFJ Wickham, SFJ Wickham, SJ White, SM Douglas, SM Douglas, TE Ouldridge, TG Cha, Y Ke, Y Okahata   +51 more
core   +1 more source