Results 41 to 50 of about 2,195,182 (275)

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

Modelling DNA-strand displacement reactions in the presence of base-pair mismatches [PDF]

, 2020
Toehold-mediated strand displacement is the most abundantly used method to achieve dynamic switching in DNA-based nanotechnology. An ‘invader’ strand binds to the ‘toehold’ overhang of a target strand and replaces a target-bound ’incumbent’ strand ...
Seidel, Ralf, Irmisch, Patrick, Ouldridge, Thomas E   +2 more
core   +1 more source

A Double-Layer Image Encryption Scheme Based on Chaotic Maps and DNA Strand Displacement

Journal of Chemistry, 2022
The image encryption schemes combining chaotic maps, DNA coding, and DNA sequence operation can effectively protect the image. In this paper, a double-layer image encryption scheme is proposed by combining chaotic maps with DNA strand displacement (DSD).
Zhen Tang, Zhixiang Yin, Risheng Wang, Xiyuan Wang, Jing Yang, Jianzhong Cui   +5 more
doaj   +1 more source

Design of DNA strand displacement reactions

Current Opinion in Biotechnology
DNA strand displacement (SD) reactions are central to the operation of many synthetic nucleic acid systems, including molecular circuits, sensors, and machines. Over the years, a broad set of design frameworks has emerged to accommodate various functional goals, initial configurations, and environmental conditions. Nevertheless, key challenges persist,
Jurinović, Križan, Mitra, Merry, Mukherjee, Rakesh, Ouldridge, Thomas E   +3 more
openaire   +3 more sources

Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

Electrochemistry Communications, 2023
In this work, a novel strand displacement polymerization strategy is designed with a single hairpin-structured DNA probe acting as both of primer and template.
Yulin Zhu, Xifeng Chen, Jiayue Shi, Haixuan Sun, Hua Chai, Peng Miao   +5 more
doaj   +1 more source

In situ reduction of gold nanoparticles-decorated MXenes-based electrochemical sensing platform for KRAS gene detection

Frontiers in Bioengineering and Biotechnology, 2023
In this work, gold nanoparticles@Ti3C2 MXenes nanocomposites with excellent properties were combined with toehold-mediated DNA strand displacement reaction to construct an electrochemical circulating tumor DNA biosensor.
Xiongtao Yu, Silan Bai, Lishi Wang
doaj   +1 more source

Irreversible DNA strand displacement.

, 2014
Irreversible DNA strand displacement.
Zhiyu Wang (282312), Tao Song (130854), Chenyan Deng (642884), Xiaolong Shi (412458), Zhihua Chen (165630), Linqiang Pan (553947)   +5 more
core   +1 more source

hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity [PDF]

, 2011
hSSB1 is a recently discovered single-stranded DNA binding protein that is essential for efficient repair of DNA double-strand breaks (DSBs) by the homologous recombination pathway.
Dinoop Menon, Cubeddu, Liza (R16726), Bain, Amanda, White, Malcolm F., Emma Bolderson, Urquhart, Aaron J., Aaron J. Urquhart, Bolderson, Emma, Khanna, Kum Kum, Menon, Dinoop, Derek J. Richard, Amanda Bain, Malcolm F. White, Richard, Derek J., Kum Kum Khanna, Liza Cubeddu, Cubeddu, Liza, White, Malcolm F.; id_orcid   +17 more
core   +2 more sources

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Controlling DNA–RNA strand displacement kinetics with base distribution [PDF]

DNA–RNA hybrid strand displacement underpins the function of many natural and engineered systems. Understanding and controlling factors affecting DNA–RNA strand displacement reactions is necessary to enable control of processes such as CRISPR-Cas9 gene ...

core   +1 more source