Results 11 to 20 of about 57,780 (287)

Type II DNA Topoisomerases Cause Spontaneous Double-Strand Breaks in Genomic DNA [PDF]

Genes, 2019
Type II DNA topoisomerase enzymes (TOP2) catalyze topological changes by strand passage reactions. They involve passing one intact double stranded DNA duplex through a transient enzyme-bridged break in another (gated helix) followed by ligation of the ...
Suguru Morimoto, M. Tsuda, Heeyoun Bunch, H. Sasanuma, C. Austin, S. Takeda   +5 more
semanticscholar   +3 more sources

Mechanism of topology simplification by type II DNA topoisomerases [PDF]

Proceedings of the National Academy of Sciences, 2001
Type II DNA topoisomerases actively reduce the fractions of knotted and catenated circular DNA below thermodynamic equilibrium values. To explain this surprising finding, we designed a model in which topoisomerases introduce a sharp bend in DNA. Because the enzymes have a specific orientation relative to the bend, they act like Maxwell's demon ...
A. Vologodskii, Wentao Zhang, V. Rybenkov, A. Podtelezhnikov, D. Subramanian, J. Griffith, N. Cozzarelli   +6 more
semanticscholar   +3 more sources

Phylogenomics of type II DNA topoisomerases

BioEssays, 2003
AbstractType II DNA topoisomerases (Topo II) are essential enzymes implicated in key nuclear processes. The recent discovery of a novel kind of Topo II (DNA topoisomerase VI) in Archaea led to a division of these enzymes into two non‐homologous families, (Topo IIA and Topo IIB) and to the identification of the eukaryotic protein that initiates meiotic ...
D. Gadelle, J. Filée, C. Buhler, P. Forterre   +3 more
semanticscholar   +3 more sources

Structure and function of type II DNA topoisomerases. [PDF]

Biochemical Journal, 1994
P. Watt, I. Hickson
semanticscholar   +3 more sources

Anaphase chromatid motion: involvement of type II DNA topoisomerases.

Biophysical Journal, 1995
Sister chromatids are topologically intertwined at the onset of anaphase: their segregation during anaphase is known to require strand-passing activity by type II DNA topoisomerase. We propose that the removal of the intertwinings involves at the same time the traction of the mitotic spindle and the activity of topoisomerases.
B. Duplantier, G. Jannink, J. Sikorav
semanticscholar   +3 more sources

Basis for the discrimination of supercoil handedness during DNA cleavage by human and bacterial type II topoisomerases.

Nucleic Acids Research, 2023
To perform double-stranded DNA passage, type II topoisomerases generate a covalent enzyme-cleaved DNA complex (i.e. cleavage complex). Although this complex is a requisite enzyme intermediate, it is also intrinsically dangerous to genomic stability ...
Jeffrey Y Jian, Kevin D. McCarty, J. A. Byl, F. Guengerich, K. Neuman, N. Osheroff   +5 more
semanticscholar   +1 more source

DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function

eLife, 2022
Type II topoisomerases modulate chromosome supercoiling, condensation, and catenation by moving one double-stranded DNA segment through a transient break in a second duplex.
Joshua Jeong, Joyce H Lee, Claudia C Carcamo, Matthew W Parker, James M Berger   +4 more
doaj   +1 more source

Viral origin of eukaryotic type IIA DNA topoisomerases

bioRxiv, 2022
Type II DNA topoisomerases of the family A (Topo IIA) are present in all bacteria (DNA gyrase) and eukaryotes. In eukaryotes, they play a major role in transcription, DNA replication, chromosome segregation and modulation of chromosome architecture.
Julien Guglielmini, Morgan Gaia, Violette Da Cunha, A. Criscuolo, M. Krupovic, P. Forterre   +5 more
semanticscholar   +1 more source

DNA-Topology Simplification by Topoisomerases

Molecules, 2021
The topological properties of DNA molecules, supercoiling, knotting, and catenation, are intimately connected with essential biological processes, such as gene expression, replication, recombination, and chromosome segregation. Non-trivial DNA topologies
Andreas Hanke, Riccardo Ziraldo, Stephen D. Levene   +2 more
doaj   +1 more source

DNA topoisomerases participate in fragility of the oncogene RET [PDF]

, 2013
Fragile site breakage was previously shown to result in rearrangement of the RET oncogene, resembling the rearrangements found in thyroid cancer. Common fragile sites are specific regions of the genome with a high susceptibility to DNA breakage under ...
A Ganguly, A Hellman, A Helmrich, AA Burrow, AJ Swerdlow, AT Jonstrup, AY Chen, BH Long, C Pondarré, CH Cheng, Christine E. Lehman, CM Caudill, D Mishmar, D Vane, DJ Mulvihill, E Grabczyk, E Zlotorynski, F de Vathaire, F Pelliccia, FH Drake, FM Bar, G Abdurashidova, G Capranico, GR Bignell, GR Sutherland, H Zhang, HY Wu, I Bongarzone, J Sambrook, JC Wang, JM Allan, JM Fortune, K Reddy, K Takeuchi, KR Hande, KW Gow, L Enewold, L Postow, Laura W. Dillon, LB Travis, Le Beau, Levi C. T. Pierce, LF Liu, LW Dillon, LW Dillon, M Drolet, M Gandhi, M Zuker, MB Smith, MD Been, MD Been, MF Arlt, MN Nikiforova, MS Kim, NR Lemoine, O Handt, P Boffetta, PA Smanik, R Garcia-Carbonero, R Venkitaraman, RT Hay, S Anderson, S Corbin, S Klugbauer, S McAvoy, S Tuduri, S Wada, SG Durkin, SG Durkin, SJ Froelich-Ammon, SM Jhiang, SM Vos, SN Shah, T Kohno, T Kondo, T Syrovets, TW Glover, TW Glover, Valentin V Rybenkov, X Li, Y Lin, Y Lin, YE Nikiforov, YE Nikiforov, YE Nikiforov, Yuh-Hwa Wang, Yuri E. Nikiforov   +86 more
core   +8 more sources