Results 41 to 50 of about 1,071,082 (162)
, 2007 Plasmids carrying the mioC promoter region, which contains two DnaA boxes, R5 and R6 with one misfit to the consensus TT(A)/(T)TNCACA, are as efficient in in vivo titration of the DnaA protein as plasmids carrying a replication-inactivated oriC region ...
Atlung, Tove +3 more
core +1 more source
DnaA Box Sequences as the Site for Helicase Delivery during Plasmid RK2 Replication Initiation in Escherichia coli [PDF]
Journal of Biological Chemistry, 2001 DnaA box sequences are a common motif present within the replication origin region of a diverse group of bacteria and prokaryotic extrachromosomal genetic elements. Although the origin opening caused by binding of the host DnaA protein has been shown to be critical for the loading of the DnaB helicase, to date there has been no direct evidence ...
M, Pacek, G, Konopa, I, Konieczny
openaire +2 more sources
Cluster of DnaA Boxes Involved in Regulation of Streptomyces Chromosome Replication: from In Silico to In Vivo Studies [PDF]
Journal of Bacteriology, 2006 ABSTRACT In Streptomyces coelicolor , replication is initiated by the DnaA protein in the centrally located oriC region and proceeds bidirectionally until the replication forks reach the ends of the linear chromosome.
Aleksandra, Smulczyk-Krawczyszyn +6 more
openaire +2 more sources
DnaA-Mediated Regulation of Phage λ-Derived Replicons in the Absence ofpRand Cro Function [PDF]
, 1998 Bacteriophage λ-derived replicons can replicate inEscherichia colicells as plasmids. In the control of replication of these plasmids, an important role was ascribed to the λ Cro repressor autoregulatory loop. However, theoR/pR-cro-tR-cII′ region could be
Herman-Antosiewicz, Anna +3 more
core +1 more source
The DnaA box R4 in the minimaloriCis dispensable for initiation ofEscherichia colichromosome replication [PDF]
Nucleic Acids Research, 1995 We have developed a genetic system with which to replace oriC+ on the Escherichia coli chromosome with modified oriC sequences constructed on plasmids. Using this system we have demonstrated that chromosomal oriC can tolerate the insertion of a 2 kb fragment at the HindIII site between DnaA boxes R3 and R4, whereas the same insertion completely ...
D B, Bates +6 more
openaire +2 more sources
Investigation of binding features between E.coli DnaA mutant proteins and DNA: Structure study of E.coli DnaA domain IV by NMR [PDF]
, 2008 The E. coli DnaA protein initiates chromosome replication and regulates gene transcription by binding to DnaA boxes, the double-strand nonamers with the consensus sequence TTA/TTNCACA.
Ma, Xiaofang
core
, 2019 DnaA is the widely conserved bacterial AAA+ ATPase that functions as both the replication initiator and a transcription factor. In many organisms, DnaA controls expression of its own gene and likely several others during growth and in response to ...
Grossman, Alan Davis +2 more
core +1 more source
Compact Origins and Where to Find Them: ORC's Guide to Genome‐Wide Licensing
BioEssays, Volume 47, Issue 7, July 2025.Origin licensing is a key requirement for any eukaryotic cell to initiate DNA replication in S‐phase. Here, we review the most recent findings in the orchestrated action of the origin recognition complex (ORC) to sequentially load two MCM2‐7 helicases onto DNA with a focus on origin architecture and sequencing‐based methods.
Christian Speck +1 more
wiley +1 more source
Comparison of DnaA boxes from a consensus sequence with DnaA boxes from the PSSM.
, 2015 (A) A logo, drawn using WebLogo [32], of the DnaA boxes used to construct the DnaA box PSSM is shown. (B-E) Histograms of the number of sequence reads that start at each nucleotide are plotted (blue for sequence reads mapping to the top strand; green for
Alan D. Grossman (237649) +1 more
core +1 more source
Bacterial transcriptional repressor NrdR – a flexible multifactorial nucleotide sensor
The FEBS Journal, Volume 292, Issue 12, Page 3091-3112, June 2025.NrdR is a bacterial transcription factor that regulates the expression of all three types of ribonucleotide reductase, an essential enzyme for cell survival, in response to intracellular ATP and dATP levels. We have carried out a comprehensive biochemical, biophysical and structural study of the response of E. coli NrdR to ATP and dATP.
Inna Rozman Grinberg +9 more
wiley +1 more source