Results 21 to 30 of about 1,071,082 (162)
Genomics, Proteomics & Bioinformatics, 2022 The replication of DNA is a complex biological process that is essential for life. Bacterial DNA replication is initiated at genomic loci referred to as replication origins (oriCs).
Mei-Jing Dong, Hao Luo, Feng Gao
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Escherichia coli DnaA Protein Loads a Single DnaB Helicase at a DnaA Box Hairpin [PDF]
Journal of Biological Chemistry, 2002 The molecular engine that drives bidirectional replication fork movement from the Escherichia coli replication origin (oriC) is the replicative helicase, DnaB. At oriC, two and only two helicase molecules are loaded, one for each replication fork. DnaA participates in helicase loading; DnaC is also involved, because it must be in a complex with DnaB ...
Kevin M, Carr, Jon M, Kaguni
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Titration of DnaA protein by oriC DnaA‐boxes increases dnaA gene expression in Escherichia coli.
The EMBO Journal, 1987 Binding of the DnaA protein to its binding sites, the DnaA-boxes (TTATCCACA), was measured by a simple physiological approach. The presence of extra DnaA-boxes in growing cells leads to a derepression of dnaA gene expression, measured as beta-galactosidase activity of a dnaA-lacZ fusion polypeptide.
F G, Hansen +3 more
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, 2022 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Weigel, Christoph +6 more
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Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box
Journal of Bacteriology, 1995 DnaA protein is a DNA-binding protein which recognizes a 9-bp consensus sequence called the DnaA box. By binding to DnaA boxes, DnaA protein regulates initiation of chromosomal replication and transcription of several genes. The dnaA gene contains two DnaA boxes, one located in the regulatory region and one within the structural gene. In this paper, we
I, Pérez-Roger +2 more
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Structure and Function of the Campylobacter jejuni Chromosome Replication Origin
Frontiers in Microbiology, 2018 Campylobacter jejuni is the leading bacterial cause of foodborne infections worldwide. However, our understanding of its cell cycle is poor. We identified the probable C.
Pawel Jaworski +5 more
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Initiation of chromosomal replication in predatory bacterium Bdellovibrio bacteriovorus
Frontiers in Microbiology, 2016 Bdellovibrio bacteriovorus is a small Gram-negative predatory bacterium that attacks other Gram-negative bacteria, including many animal, human, and plant pathogens.
Lukasz Makowski +4 more
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A Critical DnaA Box Directs the Cooperative Binding of theEscherichia coli DnaA Protein to the Plasmid RK2 Replication Origin [PDF]
Journal of Biological Chemistry, 1999 The requirement of DnaA protein binding for plasmid RK2 replication initiation the Escherichia coli was investigated by constructing mutations in the plasmid replication origin that scrambled or deleted each of the four upstream DnaA boxes. Altered origins were analyzed for replication activity in vivo and in vitro and for binding to the E.
K S, Doran, D R, Helinski, I, Konieczny
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The Box VII Motif of Escherichia coli DnaA Protein Is Required for DnaA Oligomerization at the E. coli Replication Origin [PDF]
Journal of Biological Chemistry, 2004 Escherichia coli DnaA protein initiates DNA replication from the chromosomal origin, oriC, and regulates the frequency of this process. Structure-function studies indicate that the replication initiator comprises four domains. Based on the structural similarity of Aquifex aeolicus DnaA to other AAA+ proteins that are oligomeric, it was proposed that ...
Magdalena M, Felczak, Jon M, Kaguni
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The presence of two DnaA-binding sequences is required for an efficient interaction of theEscherichia coliDnaA protein with each particular weak DnaA box region [PDF]
FEMS Microbiology Letters, 1999 Using an electron microscopic method for visualizing interactions of the Escherichia coli DnaA protein with weak DnaA-binding DNA sequences, we found that DnaA binds effectively to two separated weak DnaA box regions located on the same DNA fragment. As expected, no DnaA-DNA interactions were detected when both DnaA box regions were mutagenized to the ...
Grażyna Konopa +5 more
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