Unique and Universal Features of Epsilonproteobacterial Origins of Chromosome Replication and DnaA-DnaA Box Interactions. [PDF]
Front Microbiol, 2016 In bacteria, chromosome replication is initiated by the interaction of the initiator protein DnaA with a defined region of a chromosome at which DNA replication starts (oriC).
Jaworski P +6 more
europepmc +9 more sources
Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid [PDF]
BMC Microbiology, 2018 Background The MiniR1–1 plasmid is a derivative of the R1 plasmid, a low copy cloning vector. Results Nucleotide sequencing analysis shows that the MiniR1–1 plasmid is a 6316 bp circular double-stranded DNA molecule with an oriR1 (origin for replication).
Yuan Yao +4 more
doaj +4 more sources
Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly. [PDF]
Int J Mol Sci, 2021 oriC is a region of the bacterial chromosome at which the initiator protein DnaA interacts with specific sequences, leading to DNA unwinding and the initiation of chromosome replication.
Jaworski P +6 more
europepmc +6 more sources
Frontiers in Microbiology, 2018 More than 50 years have passed since the presentation of the Replicon Model which states that a positively acting initiator interacts with a specific site on a circular chromosome molecule to initiate DNA replication. Since then, the origin of chromosome
Flemming G. Hansen, Tove Atlung
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Regulation of chromosomal replication initiation by oriC-proximal DnaA-box clusters in Bacillus subtilis. [PDF]
Nucleic Acids Res, 2012 Bacterial chromosome replication is initiated by binding of DnaA to a DnaA-box cluster (DBC) within the replication origin (oriC). In Bacillus subtilis, six additional DBCs are found outside of oriC and some are known to be involved in tran-scriptional ...
Okumura H +5 more
europepmc +5 more sources
Mutations in the CCGTTCACA DnaA Box of Mycobacterium tuberculosis oriC That Abolish Replication of oriC Plasmids Are Tolerated on the Chromosome [PDF]
Journal of Bacteriology, 2002 The origin of replication ( oriC ) region in some clinical strains of Mycobacterium tuberculosis is a hot spot for IS 6110 elements.
Malini Rajagopalan +6 more
core +3 more sources
In-vitro helix opening of M. tuberculosis oriC by DnaA occurs at precise location and is inhibited by IciA like protein. [PDF]
PLoS ONE, 2009 BACKGROUND: Mycobacterium tuberculosis (M.tb), the pathogen that causes tuberculosis, is capable of staying asymptomatically in a latent form, persisting for years in very low replicating state, before getting reactivated to cause active infection. It is
Sandeep Kumar +2 more
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Frontiers in Microbiology, 2021 The structure and function of bacterial chromosomes are dynamically regulated by a wide variety of nucleoid-associated proteins (NAPs) and DNA superstructures, such as DNA supercoiling.
Kazutoshi Kasho +5 more
doaj +1 more source
DnaA protein binding to individual DnaA boxes in the Escherichia coli replication origin, oriC [PDF]
The EMBO Journal, 1997 The formation of nucleoprotein complexes between the Escherichia coli initiator protein DnaA and the replication origin oriC was analysed in vitro by band-shift assays and electron microscopy. DnaA protein binds equally well to linear and supercoiled oriC substrates as revealed by analysis of the binding preference to individual DnaA boxes (9-mer ...
C, Weigel +4 more
openaire +2 more sources
Development and host compatibility of plasmids for two important ruminant pathogens, Mycoplasma bovis and Mycoplasma agalactiae. [PDF]
PLoS ONE, 2015 Mycoplasma bovis is a cause of pneumonia, mastitis, arthritis and otitis media in cattle throughout the world. However, despite its clinical significance, there is a paucity of tools to genetically manipulate it, impeding our capacity to further explore ...
Shukriti Sharma +5 more
doaj +1 more source