Results 31 to 40 of about 1,071,082 (162)
A single DnaA box is sufficient for initiation from the P1 plasmid origin [PDF]
Journal of Bacteriology, 1990 The P1 plasmid replication origin requires the host DnaA protein for function. Two DnaA-binding boxes lie in tandem within the previously defined minimal origin, constituting its left boundary. Three more boxes lie 200 base pairs to the right of these, in the leader region for the P1 repA gene.
A L, Abeles, L D, Reaves, S J, Austin
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DNA replication origins in archaea
Frontiers in Microbiology, 2014 DNA replication initiation, which starts at specific chromosomal site (known as replication origins), is the key regulatory stage of chromosome replication.
Zhenfang eWu +5 more
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Host‐dependent requirement for specific DnaA boxes for plasmid RK2 replication [PDF]
Molecular Microbiology, 1999 The replication origin of the broad‐host‐range plasmid RK2, oriV, contains four DnaA boxes, which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay, mutational analysis of these boxes showed a differential requirement for replication in different Gram‐negative bacteria. DnaA boxes 3 and 4 were required in E.
K S, Doran, D R, Helinski, I, Konieczny
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Modulation of pPS10 host range by DnaA
, 2002 Narrow-host-range plasmid pPS10, originally found in Pseudomonas savastanoi, is unable to replicate in other strains such as Escherichia coli. Here, we report that the establishment of pPS10 in E.
Couturier, Martine +5 more
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Nucleic Acids Research, 1992 We have found that DnaA dependent replication of R1 still occurred when 5 of the 9 bases in the dnaA box present in oriR were changed by site directed mutagenesis although the replication efficiency decreased to 20% and 70% of the wild-type origin in vitro and in vivo respectively.
Ortega-Jiménez, S. +4 more
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DnaA Boxes Are Important Elements in Setting the Initiation Mass of Escherichia coli [PDF]
Journal of Bacteriology, 1999 ABSTRACT The binding of DnaA protein to its DNA binding sites—DnaA boxes—in the chromosomal oriC region is essential for initiation of chromosome replication. In this report, we show that additional DnaA boxes affect chromosome initiation control, i.e., increase the initiation mass.
B B, Christensen, T, Atlung, F G, Hansen
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Regulation of initiation of the chromosomal replication by DnaA‐boxes in the origin region of the Bacillus subtilis chromosome. [PDF]
The EMBO Journal, 1988 A gene homologous to the Escherichia coli dnaA gene and two flanking 'regulatory' regions which contain nine and four DnaA-boxes respectively, are located in the replication origin region of the Bacillus subtilis chromosome. Attempts to isolate an autonomously replicating fragment from these 'regulatory' regions in order to identify oriC have been ...
S, Moriya +3 more
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Novel essential residues of Hda for interaction with DnaA in the regulatory inactivation of DnaA: unique roles for Hda AAA+ Box VI and VII motifs [PDF]
Molecular Microbiology, 2010 Summary Escherichia coli ATP–DnaA initiates chromosomal replication. For preventing extra‐initiations, a complex of ADP–Hda and the DNA‐loaded replicase clamp promotes DnaA‐ATP hydrolysis, yielding inactive ADP–DnaA. However, the Hda–DnaA interaction mode remains unclear except that the Hda Box VII Arg finger (Arg‐153) and DnaA sensor II Arg‐334 within
Kenta, Nakamura, Tsutomu, Katayama
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DnaA bokses regulering af mioC promoteraktivitet [PDF]
, 2007 #DnaA is an important protein for the initiation of DNA replication and also regulates gene transcription by binding to certain sequences in the DNA called DnaA boxes.
Jovanikic, Julijana, Hockenhull, Simon
core
DNA replication‐coupled inactivation of DnaA protein in vitro: a role for DnaA arginine‐334 of the AAA+ Box VIII motif in ATP hydrolysis [PDF]
Molecular Microbiology, 2001 The DnaA protein, which initiates chromosomal replication in Escherichia coli, is negatively regulated by both the sliding clamp of DNA polymerase III holoenzyme and the IdaB protein. We have found that, when the amount of minichromosome is limited in an in vitro replication system, minichromosomal replication‐stimulated hydrolysis of DnaA‐bound ATP ...
M, Su'etsugu +5 more
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