Results 1 to 10 of about 8,074 (224)

Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis [PDF]

Scientific Reports
Sequence-specific microRNAs (miRNAs) represent promising biomarkers for tumor diagnosis, while current detection methods are often limited by stringent temperature requirements and excessive protease dependency.
Yuepeng Zhang, Yuhua Sun, Xueshan Gong, Shuang Zhao, Weiwei Cao, Hongbo Wang, Hao Wang, Xi Zhang, Changwei Du, Zhiguo Chen, Lu Deng   +10 more
doaj   +2 more sources

A Cryo-EM Method to Solve Small Nucleic Acid Structures [PDF]

Structural Dynamics
Nucleic acids adopt diverse structures that are essential to their biological function. Probing the structure-function relationship of nucleic acids provides critical insight into the principles governing molecular recognition and catalysis. DNAzymes are
Holly L. Shultz, Evan R. Cramer, Aaron R. Robart   +2 more
doaj   +2 more sources

Allergen-Induced Asthmatic Responses Modified by a GATA3-Specific DNAzyme [PDF]

New England Journal of Medicine, 2015
BACKGROUND : The most prevalent phenotype of asthma is characterized by eosinophil-dominated inflammation that is driven by a type 2 helper T cell (Th2).
Nan Zhang, Claus Bachert, Thomas Werfel
exaly   +4 more sources

Evaluation of DNA adduct damage using G-quadruplex-based DNAzyme

Bioactive Materials, 2023
Toxicity assessment is a major problem in pharmaceutical candidates and industry chemicals development. However, due to the lack of practical analytical methods for DNA adduct analysis, the safety evaluation of drug and industry chemicals was severely ...
Yi Xiao, Haomin Yi, Jingzhi Zhu, Suhua Chen, Guofang Wang, Yilong Liao, Yuanyuan Lei, Liyin Chen, Xingcai Zhang, Fangfu Ye   +9 more
doaj   +1 more source

Structure of a 10-23 deoxyribozyme exhibiting a homodimer conformation

Communications Chemistry, 2023
Deoxyribozymes (DNAzymes) are in vitro evolved DNA sequences capable of catalyzing chemical reactions. The RNA-cleaving 10-23 DNAzyme was the first DNAzyme to be evolved and possesses clinical and biotechnical applications as a biosensor and a knockdown ...
Evan R. Cramer, Sarah A. Starcovic, Rebekah M. Avey, Ali I. Kaya, Aaron R. Robart   +4 more
doaj   +1 more source

Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection

Journal of Analytical Science and Technology, 2022
Sensitive quantification of microRNA (miRNA) plays a crucial role in early diagnosis and precise therapy of osteosarcoma. Herein, we build a label-free and sensitive miRNA quantification approach based on the activation of split-DNAzyme initiated primer ...
Xuguo Luo, Hongze Wu, Mingjie Xiong, Liyun Jiang, Zhihao Jiang, Minyong Gong   +5 more
doaj   +1 more source

Studies on the Effect of Lipofectamine and Cell-Penetrating Peptide on the Properties of 10-23 DNAzyme

Molecules, 2023
Cationic polymeric materials and cell-penetrating peptides (CPPs) were often used as the delivery vectors in the evaluation of nucleic acid therapeutics.
Huanhuan Liu, Yang Li, Shanshan Du, Chenhong Wang, Yuexiang Li, Ruiyuan Cao, Weiguo Shi, Shihui Liu, Junlin He   +8 more
doaj   +1 more source

DEOXYRIBOZYMES IN DETECTION OF PATHOGENIC BACTERIA [PDF]

Biotechnologia Acta, 2021
The purpose of the review was to analyze the use of DNAzyme biosensors for the detection of pathogens. In the recent years, deoxyribozymes (DNAzymes) have a significant impact as biosensors in diverse fields, from detection of metal ions in the ...
K. A. P. Gaminda, I. B. K. Thomas, D. T. Abeysinghe, C. D. Jayasinghe , R. Senthilnithy   +4 more
doaj   +1 more source

A CRISPR/Cas12a-based DNAzyme visualization system for rapid, non-electrically dependent detection of Bacillus anthracis

Emerging Microbes and Infections, 2022
As next-generation pathogen detection methods, CRISPR-Cas-based detection methods can perform single-nucleotide polymorphism (SNP) level detection with high sensitivity and good specificity.
Dongshu Wang, Gang Chen, Yufei Lyu, Erling Feng, Li Zhu, Chao Pan, Weicai Zhang, Xiankai Liu, Hengliang Wang   +8 more
doaj   +1 more source

Programming a DNA tetrahedral nanomachine as an integrative tool for intracellular microRNA biosensing and stimulus-unlocked target regulation

Materials Today Bio, 2022
The synchronous detection and regulation of microRNAs (miRNAs) are essential for the early tumor diagnosis and treatment but remains a challenge. An integrative DNA tetrahedral nanomachine was self-assembled for sensitive detection and negative feedback ...
Lianyu Yu, Sha Yang, Zeyu Liu, Xiaopei Qiu, Xiaoqi Tang, Shuang Zhao, Hanqing Xu, Mingxuan Gao, Jing Bao, Ligai Zhang, Dan Luo, Kai Chang, Ming Chen   +12 more
doaj   +1 more source