Results 91 to 100 of about 1,575 (172)

Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome.

PLoS ONE, 2015
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants of PL1 (PL1A and PL1B) and PL9 from Clostridium thermocellum was carried out.
Soumyadeep Chakraborty, Vania O Fernandes, Fernando M V Dias, Jose A M Prates, Luis M A Ferreira, Carlos M G A Fontes, Arun Goyal, Maria S J Centeno   +7 more
doaj   +1 more source

Clostridium clariflavum: Key Cellulosome Players Are Revealed by Proteomic Analysis

mBio, 2015
Clostridium clariflavum is an anaerobic, cellulosome-forming thermophile, containing in its genome genes for a large number of cellulosomal enzyme and a complex scaffoldin system.
Lior Artzi, Ely Morag, Yoav Barak, Raphael Lamed, Edward A. Bayer   +4 more
doaj   +1 more source

Engineering an artificial catch bond using mechanical anisotropy

Nature Communications
Catch bonds are a rare class of protein-protein interactions where the bond lifetime increases under an external pulling force. Here, we report how modification of anchor geometry generates catch bonding behavior for the mechanostable Dockerin G:Cohesin ...
Zhaowei Liu, Haipei Liu, Andrés M. Vera, Byeongseon Yang, Philip Tinnefeld, Michael A. Nash   +5 more
doaj   +1 more source

Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis.

PLoS ONE, 2009
BackgroundEconomic feasibility and sustainability of lignocellulosic ethanol production requires the development of robust microorganisms that can efficiently degrade and convert plant biomass to ethanol.
Babu Raman, Chongle Pan, Gregory B Hurst, Miguel Rodriguez, Catherine K McKeown, Patricia K Lankford, Nagiza F Samatova, Jonathan R Mielenz   +7 more
doaj   +1 more source

Enzymatic profiling of cellulosomal enzymes from the human gut bacterium, Ruminococcus champanellensis, reveals a fine-tuned system for cohesin-dockerin recognition.

Environmental Microbiology, 2016
Sarah Moraïs, Yonit Ben David, Lizi Bensoussan, S. Duncan, N. Koropatkin, E. Martens, H. Flint, E. Bayer   +7 more
semanticscholar   +1 more source

Structure and enzymatic characterization of CelD endoglucanase from the anaerobic fungus Piromyces finnis. [PDF]

Appl Microbiol Biotechnol, 2023
Dementiev A, Lillington SP, Jin S, Kim Y, Jedrzejczak R, Michalska K, Joachimiak A, O'Malley MA.   +7 more
europepmc   +1 more source

Research progress of multi-enzyme complexes based on the design of scaffold protein. [PDF]

Bioresour Bioprocess, 2023
Wang X, Jiang Y, Liu H, Yuan H, Huang D, Wang T.   +5 more
europepmc   +1 more source

Cellulosome assembly and the crystal structure of the cohesin–dockerin complex [PDF]

Acta Crystallographica Section A Foundations of Crystallography, 2004
A. L. Carvalho, F. M. V. Dias, J. A. M. Prates, T. Nagy, H. J. Gilbert, G. J. Davies, C. M. G. A. Fontes, M. J. Romao   +7 more
openaire   +1 more source

Insights into lignocellulose degradation: comparative genomics of anaerobic and cellulolytic Ruminiclostridium-type species. [PDF]

Front Microbiol, 2023
You M, Zhao Q, Liu Y, Zhang W, Shen Z, Ren Z, Xu C.   +6 more
europepmc   +1 more source

Improvement of Cellulomonas fimi endoglucanase CenA by multienzymatic display on a decameric structural scaffold. [PDF]

Appl Microbiol Biotechnol, 2023
Iglesias Rando MR, Gorojovsky N, Zylberman V, Goldbaum FA, Craig PO.   +4 more
europepmc   +1 more source