Results 41 to 50 of about 1,575 (172)

Evidence for a dual binding mode of dockerin modules to cohesins [PDF]

Proceedings of the National Academy of Sciences, 2007
The assembly of proteins that display complementary activities into macromolecular complexes is critical to cellular function. One such enzyme complex, of environmental significance, is the plant cell wall degrading apparatus of anaerobic bacteria, termed the cellulosome.
Carvalho AL, Dias FMV, Nagy T, Prates JAM, Proctor MR, Smith N, Bayer EA, Davies GJ, Ferreira LMA, Romao MJ, Fontes CMGA, Gilbert HJ   +11 more
openaire   +3 more sources

Abundance and diversity of dockerin-containing proteins in the fiber-degrading rumen bacterium, Ruminococcus flavefaciens FD-1.

PLoS ONE, 2010
BackgroundThe cellulosome is a multi-enzyme machine, which plays a key role in the breakdown of plant cell walls in many anaerobic cellulose-degrading microorganisms. Ruminococcus flavefaciens FD-1, a major fiber-degrading bacterium present in the gut of
Marco T Rincon, Bareket Dassa, Harry J Flint, Anthony J Travis, Sadanari Jindou, Ilya Borovok, Raphael Lamed, Edward A Bayer, Bernard Henrissat, Pedro M Coutinho, Dion A Antonopoulos, Margret E Berg Miller, Bryan A White   +12 more
doaj   +1 more source

Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system

Biotechnology for Biofuels, 2017
Background (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B.
Olga Zhivin, Bareket Dassa, Sarah Moraïs, Sagar M. Utturkar, Steven D. Brown, Bernard Henrissat, Raphael Lamed, Edward A. Bayer   +7 more
doaj   +1 more source

Diversity and strain specificity of plant cell wall degrading enzymes revealed by the draft genome of Ruminococcus flavefaciens FD-1. [PDF]

PLoS ONE, 2009
BACKGROUND:Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides.
Margret E Berg Miller, Dionysios A Antonopoulos, Marco T Rincon, Mark Band, Albert Bari, Tatsiana Akraiko, Alvaro Hernandez, Jyothi Thimmapuram, Bernard Henrissat, Pedro M Coutinho, Ilya Borovok, Sadanari Jindou, Raphael Lamed, Harry J Flint, Edward A Bayer, Bryan A White   +15 more
doaj   +1 more source

Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme

Microorganisms, 2017
The bacterial cellulosome is an extracellular, multi-enzyme machinery, which efficiently depolymerizes plant biomass by degrading plant cell wall polysaccharides.
Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs   +6 more
doaj   +1 more source

Cohesin–dockerin interaction in cellulosome assembly: a single Asp‐to‐Asn mutation disrupts high‐affinity cohesin–dockerin binding

FEBS Letters, 2004
The cohesive cellulosome complex is sustained by the high‐affinity cohesin–dockerin interaction. In previous work [J. Biol. Chem. 276 (2001) 9883], we demonstrated that a single Thr‐to‐Leu replacement in the Clostridium thermocellum dockerin component differentiates between non‐recognition and high‐affinity recognition by the interspecies rival cohesin
Handelsman, Tal, Barak, Yoav, Nakar, David, Mechaly, Adva, Lamed, Raphael, Shoham, Yuval, Bayer, Edward A   +6 more
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Protein scaffolds: A tool for multi-enzyme assembly

Biotechnology Reports, 2021
The synthesis of complex molecules using multiple enzymes simultaneously in one reaction vessel has rapidly emerged as a new frontier in the field of bioprocess technology.
Shubhada Gad, Sonal Ayakar
doaj   +1 more source

Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization. [PDF]

Eng Life Sci
ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity ...
Ölçücü G, Jaeger KE, Krauss U.
europepmc   +2 more sources

Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii

mBio, 2015
Ruminococcus bromii is a dominant member of the human gut microbiota that plays a key role in releasing energy from dietary starches that escape digestion by host enzymes via its exceptional activity against particulate “resistant” starches.
Xiaolei Ze, Yonit Ben David, Jenny A. Laverde-Gomez, Bareket Dassa, Paul O. Sheridan, Sylvia H. Duncan, Petra Louis, Bernard Henrissat, Nathalie Juge, Nicole M. Koropatkin, Edward A. Bayer, Harry J. Flint   +11 more
doaj   +1 more source

Noncellulosomal cohesin- and dockerin-like modules in the three domains of life [PDF]

FEMS Microbiology Letters, 2009
The high-affinity cohesin-dockerin interaction was originally discovered as modular components, which mediate the assembly of the various subunits of the multienzyme cellulosome complex that characterizes some cellulolytic bacteria. Until recently, the presence of cohesins and dockerins within a bacterial proteome was considered a definitive signature ...
Ayelet, Peer, Steven P, Smith, Edward A, Bayer, Raphael, Lamed, Ilya, Borovok   +4 more
openaire   +2 more sources