Results 81 to 90 of about 1,575 (172)

Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA [PDF]

Journal of Bacteriology, 1997
This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated
E, Leibovitz, H, Ohayon, P, Gounon, P, Béguin   +3 more
openaire   +2 more sources

An extracellular glucose sensor for substrate‐dependent secretion and display of cellulose‐degrading enzymes

Biotechnology and Bioengineering, Volume 121, Issue 1, Page 403-408, January 2024.
An extracellular glucose sensor was developed for the yeast Saccharomyces cerevisiae to enable substrate‐dependent secretion and surface display of cellulases. Promoter and strain engineering increased the performance of the system, and cellobiose‐dependent signal amplification resulted in a multi‐fold increase in extracellular enzyme activity ...
Pamela B. Besada‐Lombana, Wilfred Chen, Nancy A. Da Silva   +2 more
wiley   +1 more source

Comparative analysis of carbohydrate active enzymes in Clostridium termitidis CT1112 reveals complex carbohydrate degradation ability.

PLoS ONE, 2014
Clostridium termitidis strain CT1112 is an anaerobic, gram positive, mesophilic, cellulolytic bacillus isolated from the gut of the wood-feeding termite, Nasutitermes lujae.
Riffat I Munir, John Schellenberg, Bernard Henrissat, Tobin J Verbeke, Richard Sparling, David B Levin   +5 more
doaj   +1 more source

Rumen cellulosomics: divergent fiber-degrading strategies revealed by comparative genome-wide analysis of six ruminococcal strains.

PLoS ONE, 2014
BackgroundA complex community of microorganisms is responsible for efficient plant cell wall digestion by many herbivores, notably the ruminants. Understanding the different fibrolytic mechanisms utilized by these bacteria has been of great interest in ...
Bareket Dassa, Ilya Borovok, Vered Ruimy-Israeli, Raphael Lamed, Harry J Flint, Sylvia H Duncan, Bernard Henrissat, Pedro Coutinho, Mark Morrison, Pascale Mosoni, Carl J Yeoman, Bryan A White, Edward A Bayer   +12 more
doaj   +1 more source

A high throughput approach for the generation of orthogonally interacting protein pairs

Scientific Reports, 2018
In contrast to the nearly error-free self-assembly of protein architectures in nature, artificial assembly of protein complexes with pre-defined structure and function in vitro is still challenging.
Justin Lawrie, Xi Song, Wei Niu, Jiantao Guo   +3 more
doaj   +1 more source

Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge

Biotechnology for Biofuels, 2017
Background Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass.
Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L. Nielsen, Per Halkjær Nielsen, Lene Lange   +6 more
doaj   +1 more source

Transcriptomic characterization of Caecomyces churrovis: a novel, non-rhizoid-forming lignocellulolytic anaerobic fungus

Biotechnology for Biofuels, 2017
Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates.
John K. Henske, Sean P. Gilmore, Doriv Knop, Francis J. Cunningham, Jessica A. Sexton, Chuck R. Smallwood, Vaithiyalingam Shutthanandan, James E. Evans, Michael K. Theodorou, Michelle A. O’Malley   +9 more
doaj   +1 more source

Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies.

International Journal of Biological Macromolecules, 2022
Marlene Duarte, V. Alves, Márcia A. S. Correia, C. Caseiro, L. Ferreira, M. Romão, A. Carvalho, S. Najmudin, E. Bayer, C. Fontes, P. Bule   +10 more
semanticscholar   +1 more source

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

Microbial Cell Factories, 2011
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production.
Madan Bhawna, Tsai Shen-Long, Goyal Garima, DaSilva Nancy A, Chen Wilfred   +4 more
doaj   +1 more source

Deconstruction of Lignocellulose into Soluble Sugars by Native and Designer Cellulosomes

mBio, 2012
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose, hydrophobicity of lignin, and encapsulation of cellulose by the lignin ...
Sarah Moraïs, Ely Morag, Yoav Barak, Dan Goldman, Yitzhak Hadar, Raphael Lamed, Yuval Shoham, David B. Wilson, Edward A. Bayer   +8 more
doaj   +1 more source