Results 1 to 10 of about 1,133 (97)
A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples
Gels, 2022 Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing +4 more
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Frontiers in Immunology, 2023 IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis.
Gabriel E. Wagner +13 more
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Diagnostics, 2023 Background: M2-type anti-mitochondrial autoantibodies are considered the hallmark of primary biliary cholangitis and are directed mainly against the E2 subunits of the 2-oxo acid dehydrogenase complex enzymes (PDC, BCOADC and OGDC). The aim of this study
Cristina Arnaldos-Pérez +8 more
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R-Loop Analysis by Dot-Blot [PDF]
Journal of Visualized Experiments, 2021 The three-stranded nucleic acid structure, R-loop, is increasingly recognized for its role in gene regulation. Initially, R-loops were thought to be the by-products of transcription; but recent findings of fewer R-loops in diseased cells made it clear that R-loops have functional roles in a variety of human cells. Next, it is critical to understand the
Prisila, Ramirez +3 more
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Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
Molecules, 2021 Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically ...
Marcela Vlcnovska +10 more
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Veterinary Sciences, 2018 In recent years, Brucella suis has been sporadically reported in Italy in domestic and wild swine. Since standard serological tests can determine false positive results, the development of alternative tests with improved sensitivity and specificity is ...
Fabrizio Bertelloni +7 more
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Open Life Sciences, 2023 Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use enzyme-linked immunosorbent assays (ELISA) or chemiluminescence immunoassays (CLIA); however, equipment for these immunoassays is unavailable in many areas of low- and middle ...
Apú Navilla +4 more
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Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
Bio-Protocol, 2017 N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6A antibodies and quantitative liquid ...
Lisha Shen, Zhe Liang , Hao Yu
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Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle [PDF]
Pesquisa Veterinária Brasileira, 2020 : Serological techniques can detect antibodies against Sarcocystis spp., Neospora caninum and Toxoplasma gondii antigens in single or mixed infections.
Maiara S.T. Ferreira +5 more
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