A PCR-Reverse Dot Blot Hybridization Based Microfluidics Detection System for the Rapid Identification of 13 Fungal Pathogens Directly After Blood Cultures Over a Period of Time [PDF]
Infect Drug Resist, 2023 Chunhui Lin,1,2,* Hao Tang,3,* Xinyi Hu,1,2 Ge Li,1,2 Tong Jiang,1,2 Wensu Yang,1,2 Zhaoxin Xia,1,2 Yi Zhu,1,2 Huaming Xu,4 Jing Zhou,1,2 Jilu Shen1,2 1Clinical Laboratory, the First Affiliated Hospital of Anhui Medical University, Hefei, People ...
Li G +10 more
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In-House IgM Dot-Blot Assay for Serodiagnosis of Human Leptospirosis: Development, Standardisation, and Performance Evaluation [PDF]
MicroorganismsLaboratory confirmation of human leptospirosis relies on serological tests, with the microscopic agglutination test (MAT) as the reference. However, due to its complexity, there is a need for a simpler and more accessible diagnostic method.
Roberta Morozetti Blanco +4 more
doaj +2 more sources
Development of a quick dot blot assay for the titering of bovine ephemeral fever virus [PDF]
BMC Veterinary Research, 2019 Background Bovine ephemeral fever virus (BEFV) causes fever and muscle stiffness in cattle, resulting in negative economic impact for cattle and dairy farms.
Li-Ting Cheng +3 more
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Protocol for High-Throughput Screening of Neural Cell or Brain Tissue Protein Using a Dot-Blot Technique with Near-Infrared Imaging [PDF]
STAR Protocols, 2020 Summary: Dot blotting allows for the rapid screening of a larger number of samples and/or targets than more traditional methods, such as a western blot or in-tissue-based methods.
Anna C. Chlebowski, Glen E. Kisby
doaj +2 more sources
Detection of staphylococcal enterotoxins A and B in cow milk using antigen capture enzyme-linked immunosorbent assay and dot-blot assays [PDF]
Veterinary WorldBackground and Aim: Staphylococcus aureus is a significant foodborne pathogen responsible for producing enterotoxins, particularly staphylococcal enterotoxins A (SEA) and staphylococcal enterotoxins B (SEB), which are frequently found in milk and dairy ...
Hidayatun Nisa Purwanasari +4 more
doaj +2 more sources
Assessment of Dot Blot tests and latex agglutination for cysticercosis diagnostic in Peru [PDF]
Revista Peruana de Medicina Experimental y Salud Pública, 2014 In order to evaluate dot blot tests and latex agglutination for the detection of human cysticercosis with liquid antigen of Taenia solium cysticerci, 125 human sera were used, of which 60 were from people with cysticercosis confirmed by Western Blot, 45 ...
Eduardo Miranda-Ulloa +3 more
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Development and comparison of Western blot, dot blot and ELISA for mussels tropomyosin quantification [PDF]
XI Serbian Biochemical Society Eleventh Conference, 22nd-23rd September, 2022., 2022 Tropomyosin (TPM) is considered a major allergen among different shellfish species. Developing sensitive, specific, and reliable methods for quantifying TPM in food products is crucial for persons allergic to shellfish. We have previously developed a highly sensitive sandwich ELISA method for quantifying shrimp TPM.
Radomirovic, Mirjana +3 more
openaire +6 more sources
Serological follow-up of patients with paracoccidioidomycosis treated with itraconazole using Dot-blot, ELISA and Western-blot [PDF]
Revista do Instituto de Medicina Tropical de São Paulo, 1997 Twenty-seven mycologically proven cases of paracoccidioidomycosis (PCM) were treated with itraconazole (100-200 mg/day in month 1 and 100 mg/day until month 6-8) and evaluated clinically and serologically, up to 3.5 years post-therapy, using Dot-blot and
Rosana MARTINS +4 more
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Bioconjugation of quantum dot luminescent probes for Western blot analysis [PDF]
BioTechniques, 2005 Western blot analysis is a widely used technique for protein immunodetection. Its current format, however, is unsuitable for multiplex detection of proteins, primarily due to intrinsic limitations of standard organic dyes employed as probes. Quantum dot (
Savvas C. Makride +2 more
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Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression [PDF]
BMC Biotechnology, 2004 Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu +4 more
doaj +2 more sources