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RNA interference and double-stranded-RNA-activated pathways
Biochemical Society Transactions, 2004RNAi (RNA interference) has become a powerful tool to determine gene function. Different methods of expressing the short ds (double-stranded) RNA intermediates required for interference in mammalian systems have been developed, including the introduction of si (short interfering) RNAs by direct transfection or driven from transfected plasmids or ...
C A, Sledz, B R G, Williams
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Gene silencing by double-stranded RNA
Current Opinion in Cell Biology, 2001Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21--23 nucleotides from the double-stranded RNA.
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Double-Stranded RNAs as Gene Activators
1994In 1963 Gomatos and Tamm discovered the first natural dsRNA, namely the genomic RNA of reoviruses. A few years later, Lampson et al. (1967) established that dsRNA is the active agent in an extract of a Penicillium funiculosum preparation which, following injection into rabbits, induces interferon (IFN) in serum. They also showed that various natural (i.
S, Landolfo +3 more
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The Replication of Double-Stranded RNA
1986A wide variety of fungi harbor virus-like particles (VLPs), approximately 40 nm in diameter, that contain double-stranded RNA (dsRNA) genomes (10). In some ways, these dsRNA genomes are more similar to plasmids than to true viruses. They are noninfectious and are transferred between cells only under conditions of cytoplasmic mixing, such as occurs in ...
A M, Newman, C S, McLaughlin
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Assembly of Double‐Stranded RNA Bacteriophages
2005Publisher Summary This chapter focuses on bacteriophage O6. Most of the current knowledge about the structure, assembly, and molecular biology of double stranded (ds) RNA bacteriophages is derived from model systems. It is found that the assembly of enveloped dsRNA bacteriophages can be dissected into four distinct steps: assembly of empty precursor ...
Minna M, Poranen +2 more
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Interferon stimulated by Double Stranded RNA
Nature, 1970Interferon represents an interesting approach to the control of viral disease in man and his domestic animals, as this review shows, for it is a part of the animal's own recovery processes during naturally occurring infection and active against most viruses that cause acute infection.
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Journal of Plant Research, 1999
) and wild rice (O. rufipogon) tissues. It is detected at every developmental stage, and is transmitted very efficiently to progeny via seeds (more than 98%). The dsRNA is maintained at a constant level (approximately 100 copies/cell) in almost all tissues.
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) and wild rice (O. rufipogon) tissues. It is detected at every developmental stage, and is transmitted very efficiently to progeny via seeds (more than 98%). The dsRNA is maintained at a constant level (approximately 100 copies/cell) in almost all tissues.
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Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA
Science, 2021Kun Huang, Xiaoxian Wu, Zhou-Geng Xu
exaly
การใช้ double-stranded RNA ในการพัฒนาสายพันธุ์กุ้ง
2013S1 (Special Issue 1), S.1, Thai Journal of ...
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