Results 1 to 10 of about 2,383,057 (293)

RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]

Molecular Therapy: Nucleic Acids, 2014
Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Simon J Allison, Jo Milner
doaj   +2 more sources

Alternative approach to generate shRNA from cDNA

BioTechniques, 2005
Short hairpin RNA (shRNA) synthesized from vector-based expression is as effective as short interfering RNA (siRNA) synthesized in vitro for suppressing the expression of their corresponding genes.
Anh Dinh, Yin-Yuan Mo
doaj   +1 more source

Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA

Viruses, 2020
Generation of recombinant negative-stranded RNA viruses (NSVs) from plasmids involves in vivo reconstitution of biologically active nucleocapsids and faces a unique antisense problem where the negative-sense viral genomic RNAs can hybridize to viral ...
Xiaonan Ma, Zhenghe Li
doaj   +1 more source

The rice diacylglycerol kinase family: functional analysis using transient RNA interference

Frontiers in Plant Science, 2012
Diacylglycerol kinase (DGK) is a pivotal enzyme that phosphorylates diacylglycerol (DAG) to form phosphatidic acid (PA). The production of PA from phospholipase D (PLD) and the coupled phospholipase C (PLC)/DGK route is an important signaling process in ...
Hongliang eGe, Chu eChen, Wen eJing, Qun eZhang, Hong eWang, Rong eWang, Wenhua eZhang   +6 more
doaj   +1 more source

Double-Stranded RNA Resists Condensation

Physical Review Letters, 2011
Much attention has been focused on DNA condensation because of its fundamental biological importance. The recent discovery of new roles for RNA duplexes demands efficient packaging of double-stranded RNA for therapeutics. Here we report measurements of short DNA and RNA duplexes in the presence of trivalent ions.
Li, Li, Suzette A, Pabit, Steve P, Meisburger, Lois, Pollack   +3 more
openaire   +3 more sources

Modelling evolution on design-by-contract predicts an origin of Life through an abiotic double-stranded RNA world

Biology Direct, 2007
Background It is generally believed that life first evolved from single-stranded RNA (ssRNA) that both stored genetic information and catalyzed the reactions required for self-replication. Presentation of the hypothesis By modeling early genome evolution
de Roos Albert DG
doaj   +1 more source

Sixteen Novel Mycoviruses Containing Positive Single-Stranded RNA, Double-Stranded RNA, and Negative Single-Stranded RNA Genomes Co-Infect a Single Strain of Rhizoctonia zeae. [PDF]

J Fungi (Basel), 2023
Li S, Ma Z, Zhang X, Cai Y, Han C, Wu X.
europepmc   +1 more source

Double stranded RNA sensing is silenced during early embryonic development

Nature Communications
The type I interferon response is inactive during early mammalian development and becomes functional only after gastrulation. As a result, the totipotent and pluripotent embryonic stages remain susceptible to pathogens, including viruses.
Jeroen Witteveldt, Zicong Liu, Ana Ariza-Cosano, Christian Ramirez, Jessica L. Walters, Pilar G. Marchante, Lars Maas, Elias T. Friman, Alasdair Ivens, Toma Tebaldi, Sara R. Heras, Hendrik Marks, Sara Macias   +12 more
doaj   +1 more source

NrnC, an RNase D-Like Protein From Agrobacterium, Is a Novel Octameric Nuclease That Specifically Degrades dsDNA but Leaves dsRNA Intact

Frontiers in Microbiology, 2019
NrnC from Agrobacterium tumefaciens (At_NrnC, UniProt accession number A9CG28) is a nuclease containing a single DEDDy domain. Here, we determined the structures of both the apo and metal-ion-bound forms of At_NrnC.
Zenglin Yuan, Fei Gao, Kun Yin, Lichuan Gu   +3 more
doaj   +1 more source

Unwinding and Rewinding: Double Faces of Helicase?

Journal of Nucleic Acids, 2012
Helicases are enzymes that use ATP-driven motor force to unwind double-stranded DNA or RNA. Recently, increasing evidence demonstrates that some helicases also possess rewinding activity—in other words, they can anneal two complementary single-stranded ...
Yuliang Wu
doaj   +1 more source