Results 231 to 240 of about 1,620,706 (306)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Protocol for high-content drug screening using tumor organoids on a 384-pillar plate platform. [PDF]

STAR Protoc
Choi JS, Lee MS, Song MJ, Jeong K.
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Neurofibromatosis Type 1 and the Search for Effective Tumor Therapies Using High-Throughput Drug Screening. [PDF]

Curr Oncol
Bouley SJ, Housden BE, Walker JA.
europepmc   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Development of a liquid overlay-based three-dimensional cell culture panel for drug screening applications. [PDF]

Sci Rep
Depresle M, Daumar P, Goisnard A, Roux M, Aubel C, Penault-Llorca F, Mounetou E, Bamdad M.   +7 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Patient-derived organoids based on targeted biopsy of primary prostate cancer: development, identification, and drug screening. [PDF]

Ann Med
Qian J, Peng S, Gao J, Fu Y, Huang H, Wu C, Gan W, Qiu X, Guo H.   +8 more
europepmc   +1 more source

Transcriptomics-guided high-throughput drug screening identifies potent therapies for P53 pathway altered DIPG/DMG. [PDF]

Neuro Oncol
Jiang Z, Xie L, Zhou H, Geng Y, Xiao X, Li T, Deng Y, Zhang M, Shan S, Xu C, Zhang L.   +10 more
europepmc   +1 more source