Engineering an Optogenetic pH‐Modulator in Bacteria
Advanced Science, EarlyView.ABSTRACT Cells in many naturally occurring organisms routinely cooperate to control their extracellular pH in a dynamic and reversible manner, but this capability has been underexplored in synthetic biology. Here, we sought to engineer a microbial system that switches between two states —high and low extracellular pH— with minimal human intervention ...
Jenevieve Kuang +7 more
wiley +1 more source
Optimasi Kodon dan Konstruksi Plasmid Rekombinan Protein RBD SARS-CoV-2 pada E. coli BL21(DE3)
Journal of Health (JOH)Coronavirus Disease 2019 (COVID-19), yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), telah menyebabkan lebih dari 7 juta kematian di seluruh dunia. Strategi komprehensif diperlukan untuk mengatasi pandemi ini, terutama vaksinasi untuk mengurangi infeksi lebih lanjut.
Elly Widyarni Eka Purnamasari +4 more
openaire +3 more sources
Microbial Cell FactoriesBackground Acetate is an important chemical feedstock widely applied in the food, chemical and textile industries. It is now mainly produced from petrochemical materials through chemical processes.
Jieni Zhu +15 more
doaj +1 more source
Advanced Biomedical Research, 2014 Background: For the past 30 years, bacterial L-asparaginases have been used as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. It is found in a variety of organisms such as microbes, plants and mammals.
Meraj Pourhossein, Hassan Korbekandi
doaj +1 more source
Optimization of haloacid dehalogenase production by recombinant E. coli BL21 (DE3)/pET-hakp1 containing haloacid dehalogenase gene from Klebsiella pneumoniae ITB1 using Response Surface Methodology (RSM). [PDF]
Heliyon, 2022 Ratnaningsih E +4 more
europepmc +1 more source
The pleiotropic transcriptional regulator NlpR contributes to the modulation of nitrogen metabolism, lipogenesis and triacylglycerol accumulation in oleaginous rhodococci [PDF]
, 2017 The regulatory mechanisms involved in lipogenesis and triacylglycerol (TAG) accumulation are largely unknown in oleaginous rhodococci. In this study a regulatory protein (here called NlpR: Nitrogen lipid Regulator), which contributes to the modulation of
Alvarez, Hector Manuel +4 more
core +1 more source
Advanced Science, EarlyView.A semi‐interpenetrating engineered living material (sIHSELM), fabricated via thermosensitive hydroxybutyl chitosan and covalent protein self‐assembly, demonstrates exceptional mechanical properties and environmental stability. Shielding bacteria from harsh conditions, sIHSELM exhibits remarkable efficacy in ulcerative colitis treatment and pollutants ...
Zixian Bao +8 more
wiley +1 more source
Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity. [PDF]
Heliyon, 2022 Maksum IP +5 more
europepmc +1 more source
Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System
Advanced Science, EarlyView.Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei +7 more
wiley +1 more source
Shipin gongye ke-jiThis experiment investigated the ability of an aminopeptidase gene dmpA from microcyst basidiomycete MCA 4210 (Cystobasidium minutum MCA 4210) to catalyze the synthesis of L-carnosine, and constructed a genetically engineered strain to catalyze the ...
Xiaoxuan SUN +5 more
doaj +1 more source