Results 121 to 130 of about 131,570 (298)

Cofactor Selectivity in Methylmalonyl Coenzyme A Mutase, a Model Cobamide-Dependent Enzyme. [PDF]

, 2019
Cobamides, a uniquely diverse family of enzyme cofactors related to vitamin B12, are produced exclusively by bacteria and archaea but used in all domains of life.
Mok, Kenny, Park, Jong, Quanstrom, Kathryn, Sokolovskaya, Olga, Taga, Michi, Tran, Jennifer   +5 more
core   +1 more source

G4‐Ligand‐Directed PROTACs Unveil DR1 as a Novel Ligand‐Co‐Binding G4‐Protein and Reshape G4‐Dependent Transcription

Advanced Science, EarlyView.
G4‐binding proteins (G4BPs) that specifically co‐bind G4s in the presence of small‐molecule ligands represent a critical but unexplored class of proteins. We introduce G4‐Ligand‐Directed PROTACs (G4L‐TACs), a chemical platform that couples G4 ligands (PDS) to E3 recruiters to selectively degrade these ligand‐co‐binding G4BPs.
Mao‐Lin Li, Shu‐Min Xu, Xin‐Chen Jiang, Le‐Tian Dai, Yu‐Tao Hu, Kai‐Bo Wang, Jia‐Heng Tan, Zhi‐Shu Huang, Shuo‐Bin Chen   +8 more
wiley   +1 more source

To Construct an Engineered (S)-Equol Resistant E. coli for in Vitro (S)-Equol Production

Frontiers in Microbiology, 2018
(S)-equol is one of the major metabolites of daidzein that is produced by human and animal gut bacteria. Most of the physiological functions of soybean isoflavones, such as anti-oxidative activity, anti-cancer activity, and cardiovascular protection have
Hailiang Li, Hailiang Li, Hailiang Li, Shaoming Mao, Huahai Chen, Huahai Chen, Liying Zhu, Wei Liu, Xin Wang, Yeshi Yin, Yeshi Yin   +10 more
doaj   +1 more source

Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration [PDF]

, 2009
Background Thermostable enzymes from thermophilic microorganisms are playing more and more important roles in molecular biology R&D and industrial applications.
Yiran Wang, Y-H Percival Zhang
core   +1 more source

Introgressed Variation in TaMYB7‐A1 Drives Graded Dormancy and Climate‐Adaptive Pre‐Harvest Sprouting Resistance in Wheat

Advanced Science, EarlyView.
TaMYB7‐A1 directly activates TaABI5 to enhance ABA signaling and regulate ABA‐GA homeostasis, enforcing seed dormancy. Its superior allele, derived from wild einkorn introgression, harbors a MITE insertion that elevates expression and two amino acid substitutions that enhance transcriptional activity, collectively generating graded PHS resistance for ...
Hao Wang, Dongzhi Wang, Yafei Guo, Qi Zheng, Min Zhang, James Simmonds, Jian Hou, Xuemei Liu, Xuelei Lin, Xiaomin Bie, Xiansheng Zhang, Xiaohui Li, Yuan Chen, Xueyong Zhang, Cristobal Uauy, Fei Lu, Chengcai Chu, Zhixi Tian, Jun Xiao   +18 more
wiley   +1 more source

Sucrose as an electron source for cofactor regeneration in recombinant Escherichia coli expressing invertase and a Baeyer Villiger monooxygenase

Microbial Cell Factories
Background The large-scale biocatalytic application of oxidoreductases requires systems for a cost-effective and efficient regeneration of redox cofactors. These represent the major bottleneck for industrial bioproduction and an important cost factor. In
Lucija Sovic, Lenny Malihan-Yap, Gábor Szilveszter Tóth, Vilja Siitonen, Véronique Alphand, Yagut Allahverdiyeva, Robert Kourist   +6 more
doaj   +1 more source

Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3). [PDF]

Appl Microbiol Biotechnol, 2021
Jones CM, Korosh TC, Nielsen DR, Pfleger BF.   +3 more
europepmc   +1 more source

Novel frataxin isoforms may contribute to the pathological mechanism of friedreich ataxia [PDF]

, 2012
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
Di Zhou, Efthimios M. C. Skoulakis, Haiyan Xia, Kuanyu Li, Mark A. Pook, Ran Mo, Sahar Al-Mahdawi, Silke Leimkühler, Tracey A. Rouault, Xiaoman Dai, Yun Cao, Zvonimir Marelja   +11 more
core   +4 more sources

CRISPR/Cas13a: Compensatory Target Activation Mechanism

Advanced Science, EarlyView.
CRISPR/Cas13a‐CTAM as a novel compensatory target activation mechanism that enables synergistic activation of Cas13a via two independently editable short RNA effectors. This dual‐effector system maintains enzymatic activity comparable to traditional single‐effector while significantly enhancing flexibility, sensitivity, and application scope.
Bowen Jiang, Tenghua Zhang, Yao Lu, Shujun Zhou, Wenjing Xiao, Shuming Pan, Nan Shi, Yan Sheng, Jiaming Hu   +8 more
wiley   +1 more source

Engineering E. coli recombinant strains for high yield production of Burkholderia pseudomallei specific antigens

Инфекция и иммунитет, 2019
A risk of introducing into the Russian Federation exotic infections including laboratory-confirmed melioidosis regularly recorded worldwide necessitates development and improvement of express diagnostics tools.
Yu. A. Kuzyutina, I. B. Zakharova, D. V. Viktorov   +2 more
doaj   +1 more source