Results 121 to 130 of about 92,801 (255)

Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy. [PDF]

Int J Mol Sci, 2020
Schwaighofer A, Ablasser S, Lux L, Kopp J, Herwig C, Spadiut O, Lendl B, Slouka C.   +7 more
europepmc   +1 more source

UFL1‐Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression

Advanced Science, EarlyView.
UFMylation restrains aerobic glycolysis and colorectal cancer progression by modifying the glycolytic enzyme ENO1. Pharmacological enhancement of the UFL1–ENO1 interaction by the FDA‐approved antibiotic torezolid promotes ENO1 UFMylation, suppresses tumor metabolism, and sensitizes colorectal cancer to chemotherapy, revealing an actionable metabolic ...
Xiuqing Ma, Rui Wan, Yueyuan Zhong, Ziyang Cui, Xuan Zhang, Xiaoqiang He, Rong Wang, Lei Huang, Wan‐Yang Sun, Rong‐Rong He, Yang Zhou, Jianshuang Li, Li Shen, Shao‐Hua Wang, Tongzheng Liu   +14 more
wiley   +1 more source

F‐Type Pyocin Versus Phage λ Tail: Conserved Hub, Divergent Fibers

Advanced Science, EarlyView.
Near‐atomic cryo‐EM structures of a Gram‐negative F‐type pyocin reveal a conserved tail tip architecture shared with bacteriophage λ together with a previously unrecognized side‐fiber attachment mode. These findings provide new insight into tailocin assembly and host recognition and establish a structural framework for engineering precision ...
Zhiwei Gu, Yufan Xie, Lanxin Wang, Luyan Ma, Xiaofei Ge, Jiawei Wang   +5 more
wiley   +1 more source

Accelerating Biosensor Discovery: A Computationally‐Driven Pipeline for Microplastics Monitoring

Advanced Intelligent Discovery, EarlyView.
A computationally guided pipeline unites molecular simulation, synthetic biology, electrochemical engineering, and machine learning to accelerate biosensor discovery. A Bacillus anthracis carbohydrate‐binding module is used to develop a high‐performance micro‐ and nanoplastics sensor with greatly reduced error and variability.
Gabriel X. Pereira, Marcos de C. Leite, Pedro H. M. Zanineli, Juliana N. Y. Costa, Débora M. Cunha, Sophia P. Serra, Pedro H. Sophia, Flávio M. Shimizu, Yasmin W. Moura, Célio C. Oliveira, Gabriel R. Schleder, Gabriela F. Persinoti, Leandro Merces, Renato S. Lima   +13 more
wiley   +1 more source

Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3). [PDF]

Bioengineering (Basel), 2017
Kopp J, Slouka C, Ulonska S, Kager J, Fricke J, Spadiut O, Herwig C.   +6 more
europepmc   +1 more source

Rewriting Tumor Immunity With Bacterial Membrane Vesicles: A Versatile and Potent Therapeutic Strategy

Advanced NanoBiomed Research, EarlyView.
Bacterial membrane vesicles (BMVs) emerge as promising platforms for cancer immunotherapy owing to their intrinsic adjuvant properties and tunable cargo delivery capabilities. Their ability to modulate the tumor microenvironment, enhance antitumor immune responses, and support personalized therapeutic strategies highlights their growing potential as ...
Md Sifat Rahi, Md Mijanur Rahman, Asma Talukder, Urooj Yousaf Virk, Jennifer C. Wilson, Nigel McMillan, Ming Q. Wei   +6 more
wiley   +1 more source

Design and Construction of ctxB-gfp-stxB Gene Cassette and Investigation of Its Expression in E. coli Bl21 (DE3)

Journal of Fasa University of Medical Sciences, 2013
Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future
Aghil Esmaeili, Hossein Honari, Sadegh Safaei, Mahdi Ghofrani   +3 more
openaire   +1 more source

Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)

New Biotechnology
Y Wang, S Leontakianakou, E Nordberg Karlsson, W van Gulik   +3 more
openaire   +1 more source

Cloning and Recombinant Expression of Intimin C-Terminal Domain of EHEC Pathogen in E. coli BL21(DE3) Strain

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2015
Abstract   Background and Objectives: Some intestinal infections are caused by pathogens that enter the gastrointestinal tract of humans by consumption of contaminated food. A set of proteins are involved in colonization of E. coli O157:H7 in the large intestine, some of which initiate this phenomenon and their absence prevents it.
Mostafa Bakhshi, Firouz Ebrahimi, Vahide Sheikhzade   +2 more
openaire   +1 more source

The sRNA vvrr1 Modulates the VarS/VarA‐Csr System via LeuO to Regulate Biofilm Formation and Virulence in Vibrio alginolyticus

Animal Research and One Health, EarlyView.
vvrr1, a small RNA, post‐transcriptionally represses the transcriptional regulator LeuO, which directly inhibits the VarS/VarA two‐component system. This triggers a Csr cascade that relieves LuxO repression, ultimately modulating exopolysaccharide production to control biofilm formation and virulence in Vibrio alginolyticus.
Theerakamol Pengsakul, Lei Wen, Lingmin Zhao, Bei Huang, Jiang Zheng, Binfu Xu, Lixing Huang   +6 more
wiley   +1 more source