Results 101 to 110 of about 131,570 (298)

Cloning and Expression of Recombinant Human Interleukin 1 Receptor Antagonist in Escherichia Coli Strains, BL21 (DE3), Rosetta (DE3), and Origami (DE3)

مجله دانشکده پزشکی اصفهان, 2019
Background: Recombinant human interleukin-1 receptor antagonist (IL-1RA) is a protein with 153 amino acids and molecular weight of 16.83 kDa. This drug protein is known as Anakinra, and has an effective application in the treatment of rheumatoid ...
Hanieh Fardgoli, Seyed Nezameddin Hoseini, Setareh Haghighat   +2 more
doaj   +1 more source

Characterization of GDP-mannose Pyrophosphorylase from Escherichia Coli O157:H7 EDL933 and Its Broad Substrate Specificity

, 2005
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed
Hwang, Bum-Yeol, Kang, Young Bok (Abraham), Kim, Byung-Gee, Lee, Kwang-Won, Lee, Tek-Hyung, Park, Sung-Soo, Yang, Yung-Hun   +6 more
core  

Function of the Signal Peptide and N- and C-terminal Propeptides in the Leucine Aminopeptidase from \u3cem\u3eAeromonas proteolytica\u3c/em\u3e [PDF]

, 2004
The leucine aminopeptidase from Aeromonas proteolytica (also known as Vibrio proteolyticus) (AAP) is a metalloenzyme with broad substrate specificity. The open reading frame (ORF) for AAP encodes a 54 kDa enzyme, however, the extracellular enzyme has a ...
Bzymek, Krzysztof P., Campbell, Heidi, Chen, Guanjing, D\u27Souza, Ventris M., Holz, Richard C., Mitchell, Alice   +5 more
core   +1 more source

Constructing and testing the thermodynamic limits of synthetic NAD(P)H:H-2 pathways [PDF]

, 2008
NAD(P)H:H(2) pathways are theoretically predicted to reach equilibrium at very low partial headspace H(2) pressure. An evaluation of the directionality of such near-equilibrium pathways in vivo, using a defined experimental system, is therefore important
Akhtar, Akhtar, Alberty, Alexeeva, Andersen, Angenent, Bautista, Blaschkowski, Brumaghim, Carrillo, Cho, Datsenko, De Vrije, Fischer, Fitzgerald, Gorwa, Gottschalk, Graves, Henry, Jungermann, Jungermann, Kimball, King, Kumar, Lamed, Lee, Lovley, Maeda, Malki, Meyer, Miller, Nakashimada, Patterson, Pauss, Pollak, Posewitz, Rabinowitz, Sambrook, Sauer, Sawers, Seo, Seo, Serres, Silva, Soboh, Steuber, Thauer, Van Groenestijn, Van Niel, Varma, Verhagen, Vignais, Walton, Woodward, Wu, Yoshida, Yoshida   +56 more
core   +1 more source

Identifying Cytokine Motif‐Containing, Immunomodulatory Bacterial Proteins in Human Gut Microbiome

Advanced Science, EarlyView.
By building and constructing HMM (Upper left, blue), the authors identify CMCPs in bacteria genomes and CRC related metagenomes and enriched CRC‐related CMCPs (Upper right, blue). They analyze sequence and structural similarity of hits (Lower left, green), test function with engineered EcN delivered to tumors in a mouse tumor model (Lower right, pink ...
Ziyu Wang, Siqi Guo, Jing Li, Qianqian Huang, Jing Ning, Binbin Xia, Xun Lv, Xiang Liu, Zeyu Gao, Jine Li, Longchao Liu, Moshi Song, Jun Wang   +12 more
wiley   +1 more source

Characterization of the genome editing with miniature DNA nucleases TnpB and IscB in Escherichia coli strains

Communications Biology
DNA endonucleases TnpB and IscB are emerging candidates for combating drug-resistant bacteria, particularly Escherichia coli, due to their specificity in targeting DNA and smaller size. However, the genome-editing of TnpB/IscB in E. coli remains unclear.
Hongjie Tang, Jie Gao, Hengyi Wang, Mingjun Sun, Suyi Zhang, Chuan Song, Qi Li   +6 more
doaj   +1 more source

High Expression Achievement of Active and Robust Anti-β2 microglobulin Nanobodies via E.coli Hosts Selection

Molecules, 2019
Nanobodies (VHHs) overcome many of the drawbacks of conventional antibodies, and the related technologies represent state-of-the-art and advanced applications in scientific research, pharmaceuticals, and therapies.
Da Li, Fangling Ji, Chundong Huang, Lingyun Jia   +3 more
doaj   +1 more source

Investigation of the biosynthesis of exopolysaccharides within the biofilm matrix of Pseudomonas aeruginosa and Pseudomonas syringae pv. actinidiea : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology and Genetics at Massey University, Manawatu, New Zealand [PDF]

, 2017
Polysaccharides are highly abundant natural biopolymers, which have biologically significant structural functions in living organisms. Various polysaccharides, with specific physicochemical properties, contribute to biofilm formation; defined as cell ...
Ghods, Shirin
core  

Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions [PDF]

, 2017
This work was supported in part by the European Union, Area NMP.2013.1.1–2: Self-assembly of naturally occurring nanosystems: CellulosomePlus Project number: 604530, and by the EU Seventh Framework Programme (FP7 2007–2013) under the WallTraC project ...
Alves, Victor D, Barak, Yoav, Bayer, Edward A, Borovok, Ilya, Bule, Pedro, Cardoso, Vânia, Dassa, Bareket, Flint, Harry J, Fontes, Carlos M G A, Gilbert, Harry J, Hamberg, Yuval, Israeli-Ruimy, Vered, Jindou, Sadanari, Lamed, Raphael, Moraïs, Sarah, Najmudin, Shabir, Slutzki, Michal, White, Bryan A   +17 more
core   +1 more source

D-Xylonic Acid from Recombinant E. coli BL21 (DE3): Comparison between Shake Flask and Benchtop Bioreactor Fermentation

Journal of Physics: Conference Series, 2020
Abstract Production D-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of D-xylonic acid in both
N A M Rodzri, W S W M Zain, R M A Hanapiah, R A Samah, R M Illias   +4 more
openaire   +1 more source