Results 81 to 90 of about 131,570 (298)

Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli [PDF]

, 2012
BACKGROUND: 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens.
Jin-Ho Seo, Josh Quarterman, Jung-Hyun Jo, Michael J Miller, Nam Han, Panchalee Pathanibul, Won-Heong Lee, Yong-Su Jin   +7 more
core   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

De-convoluting the Genetic Adaptations of E. coli C41(DE3) in Real Time Reveals How Alleviating Protein Production Stress Improves Yields

Cell Reports, 2015
The well-established E. coli protein production strain C41(DE3) was isolated from the T7 RNA polymerase-based BL21(DE3) strain for its ability to produce difficult recombinant proteins, and it acquired multiple mutations during its isolation.
Susan Schlegel, Pierre Genevaux, Jan-Willem de Gier   +2 more
doaj   +1 more source

Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways [PDF]

, 2019
Background The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity ...
Argandoña Bertrán, Montserrat, Bernal, Vicente, Borges, Nuno, Castaño Cerezo, Sara, Csonka, Laszlo N., Cánovas, Manuel, Gonçalves, Luís G., Goodner, Bradley W., Nieto Gutiérrez, Joaquín José, Pagán, Juan P., Pastor, José M., Reynolds, Kathryn A., Vargas Macías, Carmen   +12 more
core   +1 more source

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

Advanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
wiley   +1 more source

Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyase

Microbial Cell Factories, 2005
Background Production of heterologous proteins in the E. coli periplasm, or into the extracellular fluid has many advantages; therefore naturally occurring signal peptides are selected for proteins translocation.
Trikka Fotini A, Chaitidou Sotiria A, Papi Rigini M, Kyriakidis Dimitrios A   +3 more
doaj   +1 more source

In Vitro Stability of Phytase from Recombinant Bacteria E. Coli BL21 (DE3) EAS1-AMP [PDF]

, 2011
The objective of the research was to inquire the Km, Vm, activity, intracellular phytase stability exposed to pH variation, temperature variation and protease (pepsin and pancreas) in vitro.
Bachruddin, Z., Nuhriawangsa, A. M. P., SAJIDAN,, Wibowo, A.   +3 more
core  

Expansion of Thaumarchaeota habitat range is correlated with horizontal transfer of ATPase operons. [PDF]

, 2019
Thaumarchaeota are responsible for a significant fraction of ammonia oxidation in the oceans and in soils that range from alkaline to acidic. However, the adaptive mechanisms underpinning their habitat expansion remain poorly understood.
Bartlett, Douglas H, Eloe-Fadrosh, Emiley A, Hall, Richard, Han, Ping, Heiner, Cheryl, Herbold, Craig W, Huang, Li, Ingalls, Anitra E, Ji, Junbin, Jia, Zhongjun, Jung, Man-Young, Li, Meng, Liu, Linmeng, Liu, Yang, Lu, Lu, Martens-Habbena, Willm, Qin, Wei, Ren, Yi, Rhee, Sung-Keun, Stahl, David A, Wagner, Michael, Wang, Baozhan, Yan, Xin, Zheng, Yue, Zhou, Xue   +24 more
core   +2 more sources

Mass Spectrometry Imaging‐Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa

Advanced Science, EarlyView.
An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu, Wenna Duan, Weiwei Tang, Yucheng Zhao, Bin Li   +4 more
wiley   +1 more source

A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate

BMC Microbiology, 2020
Background A broad range of aromatic compounds can be degraded by enteric bacteria, and hydroxyphenylacetic acid (HPA) degrading bacteria are the most widespread.
Hanseol Kim, Sinyeon Kim, Dohyeon Kim, Sung Ho Yoon   +3 more
doaj   +1 more source