Results 81 to 90 of about 92,801 (255)

To Construct an Engineered (S)-Equol Resistant E. coli for in Vitro (S)-Equol Production

Frontiers in Microbiology, 2018
(S)-equol is one of the major metabolites of daidzein that is produced by human and animal gut bacteria. Most of the physiological functions of soybean isoflavones, such as anti-oxidative activity, anti-cancer activity, and cardiovascular protection have
Hailiang Li, Hailiang Li, Hailiang Li, Shaoming Mao, Huahai Chen, Huahai Chen, Liying Zhu, Wei Liu, Xin Wang, Yeshi Yin, Yeshi Yin   +10 more
doaj   +1 more source

NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote Obesity

Advanced Science, EarlyView.
Adipose tissue‐derived NMI is secreted under metabolic stress and suppresses adaptive thermogenesis through TLR4/IRF3 signaling, repressing the PPARα/PGC‐1α/UCP1 thermogenic transcriptional program. Genetic ablation or anti ‐ NMI monoclonal antibody treatment enhances energy expenditure, protects against DIO, and ameliorates adipose tissue inflammation,
Ting‐Ting Li, Xin‐Yuan Zhao, Min Zhang, Zhuang‐Feng Weng, Xiao‐Ran Guo, Ying‐Fang Liu, Qiao‐Ping Wang, Huan‐Huan Liang   +7 more
wiley   +1 more source

Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3). [PDF]

Appl Microbiol Biotechnol, 2021
Jones CM, Korosh TC, Nielsen DR, Pfleger BF.   +3 more
europepmc   +1 more source

Expression and Characterization of Recombinant PETase in E. coli BL21(DE3) for PET Plastic Degradation

Asian Journal of Biochemistry, Genetics and Molecular Biology
Aim: Plastic pollution caused by the persistence of polyethylene terephthalate (PET) has become a major environmental challenge due to its resistance to natural degradation. Biocatalytic degradation using PETase provides an eco-friendly and sustainable solution for PET recycling.
openaire   +2 more sources

Discriminator‐Guided Inverse Folding for Multi‐Property Protein Design

Advanced Science, EarlyView.
Discriminator‐Guided Inverse Folding (DGIF) integrates multiple property predictors trained from single‐property datasets to guide protein sequence generation from a backbone structure. DGIF enables simultaneous improvement of thermostability and solubility without requiring multi‐property annotated datasets and generates designs that move toward the ...
Yuchuan Zheng, Chuyi Liu, Zhaoming Liu, Mao Su, Chenyu Tang, Xiang Zheng, Hao Zhang, Jingyuan Li   +7 more
wiley   +1 more source

Engineering E. coli recombinant strains for high yield production of Burkholderia pseudomallei specific antigens

Инфекция и иммунитет, 2019
A risk of introducing into the Russian Federation exotic infections including laboratory-confirmed melioidosis regularly recorded worldwide necessitates development and improvement of express diagnostics tools.
Yu. A. Kuzyutina, I. B. Zakharova, D. V. Viktorov   +2 more
doaj   +1 more source

RNF138‐Mediated Ubiquitination and Degradation of NS5 Restricts Tick‐Borne Encephalitis Virus Infection

Advanced Science, EarlyView.
Host‐specific compatibility between RNF138‐like proteins and flavivirus NS5 determines NS5 stability. Mammalian RNF138 but not arthropod homologs recognizes and induces conserved NS5/RdRp K48‐linked ubiquitination and proteasomal degradation, thereby restricting viral replication. Ectopic RNF138 in mice attenuates TBEV‐induced pathogenesis. (Created in
Jialiang Sun, Weijing Yang, Hao Zhou, Shuai Li, Xin Jiang, Jianyang Gu, Wenyan Zhang   +6 more
wiley   +1 more source

Acetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway

Microbial Cell Factories
Background Acetate is an important chemical feedstock widely applied in the food, chemical and textile industries. It is now mainly produced from petrochemical materials through chemical processes.
Jieni Zhu, Wei Liu, Leilei Guo, Xiaoxu Tan, Weikang Sun, Hongxu Zhang, Hui Zhang, Wenjia Tian, Tianyi Jiang, Wensi Meng, Yidong Liu, Zhaoqi Kang, Chao Gao, Chuanjuan Lü, Ping Xu, Cuiqing Ma   +15 more
doaj   +1 more source

Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli

Advanced Biomedical Research, 2014
Background: For the past 30 years, bacterial L-asparaginases have been used as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. It is found in a variety of organisms such as microbes, plants and mammals.
Meraj Pourhossein, Hassan Korbekandi
doaj   +1 more source

Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity. [PDF]

Heliyon, 2022
Maksum IP, Yosua Y, Nabiel A, Pratiwi RD, Sriwidodo S, Soedjanaatmadja UMS.   +5 more
europepmc   +1 more source