Results 71 to 80 of about 131,570 (298)

High energy-charged cell factory for heterologous protein synthesis [PDF]

, 2009
Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide ...
Hye Jung Kim, Pil Kim, Sang Yup Lee, Yeong Deok Kwon   +3 more
core   +1 more source

\u3cem\u3eArabidopsis thaliana\u3c/em\u3e GLX2-1 Contains a Dinuclear Metal Binding Site, but Is Not a Glyoxalase 2 [PDF]

, 2009
In an effort to probe the structure and function of a predicted mitochondrial glyoxalase 2, GLX2-1, from Arabidopsis thaliana, GLX2-1 was cloned, overexpressed, purified and characterized using metal analyses, kinetics, and UV–visible, EPR, and 1H-NMR ...
Bennett, Brian, Crowder, Michael W., Limphong, Pattraranee, Makaroff, Christopher A.   +3 more
core   +2 more sources

Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane [PDF]

, 2015
Lipopolysaccharide (LPS) is a main component of the outer membrane of Gram-negative bacteria, which is essential for the vitality of most Gram-negative bacteria and plays a critical role for drug resistance.
AX Tran, B van den Berg, C Dong, C Whitfield, CC Liu, DJ Sherman, E Freinkman, E Freinkman, EM Hearn, G Malojcic, G Zhang, H Dong, H Hong, H Liu, H Mori, JC Kauer, JC Whitney, JW Chin, KM Schultz, M Grabowicz, MA Khan, MD Suits, N Ruiz, NN Nickerson, P Sperandeo, P Sperandeo, QJ Xiang, R Villa, RE Bishop, RE Bishop, S Okuda, S Okuda, S Qiao, TS Young, Y Gu, Z Wang   +35 more
core   +1 more source

Coacervates Made of Elastin‐Like Polypeptides Fused with Melanocyte‐Stimulating Hormone and Monocyte Chemoattractant Protein Enhance Skin Wound Healing in Spinal Cord‐Injured Mice

Advanced Healthcare Materials, EarlyView.
Pressure skin wounds are frequent complications after spinal cord injury (SCI), with impaired healing due to vascular and immune deficits. Elastin‐like polypeptides (ELP) fused to α‐MSH (MSH‐ELP) or MCP‐1 (MCP‐ELP) are developed and tested on these wounds. The resulting nanoparticles are non‐toxic and bioactive, and they enhance macrophage recruitment,
Suneel Kumar, Dhruv Patel, Greeshma Manjunath, Shashank Madhavan, Ashley Mathew, Dnyaneshwari Rananavare, Jahnavi Shah, Francois Berthiaume   +7 more
wiley   +1 more source

Construction of an Antibiotic-Free Vector and its Application in the Metabolic Engineering of Escherichia Coli for Polyhydroxybutyrate Production

Frontiers in Bioengineering and Biotechnology, 2022
An antibiotic- and inducer-free culture condition was proposed for polyhydroxybutyrate (PHB) production in recombinant Escherichia coli. First, antibiotic-free vectors were constructed by installing the plasmid maintenance system, alp7, hok/sok, and the ...
Ying-Cheng Liao, Boonyawee Saengsawang, Jun-Wei Chen, Xiao-Zhen Zhuo, Si-Yu Li, Si-Yu Li   +5 more
doaj   +1 more source

The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein [PDF]

, 2007
Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777.
Abella, M, Barbe, J, Campoy, S, Paytubi, S, Rodriquez, S, White, Malcolm Frederick   +5 more
core   +1 more source

Optimised Expression and Activity Assessment of Bacterial Staphylokinase in E. coli BL21(DE3)

, 2023
Abstract The application of recombinant proteins is rare following the high production costs of expressing proteins with expensive inducers, such as isopropyl-β-D-1-thiogalactopyranoside (IPTG). Staphylokinase (SAK), a fibrinolytic enzyme, is a small bacterial thrombolytic agent that specifically clots and converts plasminogens to plasmins and ...
openaire   +1 more source

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

ADP-dependent Phosphofructokinases in Mesophilic and Thermophilic Methanogenic Archaea [PDF]

, 2001
Phosphofructokinase (PFK) is a key enzyme of the glycolytic pathway in all domains of life. Two related PFKs, ATP-dependent and PPi-dependent PFK, have been distinguished in bacteria and eucarya, as well as in some archaea.
Verhees, C.H., Tuininga, J.E., Kengen, S.W.M., Stams, A.J.M., Oost, J., van der, Vos, W.M., de   +5 more
core   +1 more source

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source