Results 71 to 80 of about 92,801 (255)

Characterization of the genome editing with miniature DNA nucleases TnpB and IscB in Escherichia coli strains

Communications Biology
DNA endonucleases TnpB and IscB are emerging candidates for combating drug-resistant bacteria, particularly Escherichia coli, due to their specificity in targeting DNA and smaller size. However, the genome-editing of TnpB/IscB in E. coli remains unclear.
Hongjie Tang, Jie Gao, Hengyi Wang, Mingjun Sun, Suyi Zhang, Chuan Song, Qi Li   +6 more
doaj   +1 more source

High Expression Achievement of Active and Robust Anti-β2 microglobulin Nanobodies via E.coli Hosts Selection

Molecules, 2019
Nanobodies (VHHs) overcome many of the drawbacks of conventional antibodies, and the related technologies represent state-of-the-art and advanced applications in scientific research, pharmaceuticals, and therapies.
Da Li, Fangling Ji, Chundong Huang, Lingyun Jia   +3 more
doaj   +1 more source

Streptavidins Coordinate Biotin Sequestration and Self‐Resistance Within a Biotin‐Pathway Antibiotic Network

Advanced Science, EarlyView.
A conserved genomic region between two streptavidin genes in Streptomyces packages biosynthetic gene clusters for diverse biotin‐pathway antibiotics: acidomycin, stravidin, the new non‐proteinogenic amino acid ANDA, and the new BioA inhibitor α‐methyl‐KAPA.
Sumire Kurosawa, Jingjun Mo, Xiaotong Diao, Feng Xie, Tingting Wang, Haowen Zhao, Dalei Wu, Rolf Müller, Chengzhang Fu   +8 more
wiley   +1 more source

Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity

Advanced Science, EarlyView.
STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang, Yuanyuan Chu, Ruiqian Zhao, Siyu Li, Yu Feng, Hongdan Zheng, Yunli Xie, Tong Wang, Wenyu Wen   +8 more
wiley   +1 more source

Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli

Hayati Journal of Biosciences, 2016
Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available.
Hamdayanty Hamdayanty, Sri Hendrastuti Hidayat, Tri Asmira Damayanti   +2 more
doaj   +1 more source

Protein Structuromics Reveals a Loop‐Controlled Half‐Open Active Pocket Conformation Throughout Fe(II)/α‐ketoglutarate‐Dependent Dioxygenase Catalytic Cycle

Advanced Science, EarlyView.
This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu, Huan Liu, Songyin Zhao, Lei Qin, Jiarui Li, Jun Wang, Zixuan Dai, Jie Gu, Yan Xu, Feiran Li, Yao Nie   +10 more
wiley   +1 more source

High-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy.

Iranian journal of pharmaceutical research : IJPR, 2019
A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1 ...
Malekian, Razieh, Jahanian-Najafabadi, Ali, Moazen, Fatemeh, Ghavimi, Reza, Mohammadi, Elmira, Akbari, Vajihe   +5 more
openaire   +2 more sources

Histone Modification Complex JMJ704‐HDA709 Negatively Regulates Salinity Tolerance in Rice

Advanced Science, EarlyView.
This study reveals that the rice histone demethylase JMJ704 interacts with HDA709―a H3K9ac deacetylase characterized herein―to form a chromatin‐modifying complex. Under salt stress, OsWRKY72 recruits this complex through interaction with JMJ704 to target loci, repressing the expression of oxidative stress and salt‐responsive genes via removal of ...
Jing Wang, Zhengting Chen, Pengxiang Bai, Biao Liu, Lingling Meng, Jian Zhang, Xu Li, Dao‐Xiu Zhou, Xuan Ma, Yu Zhao   +9 more
wiley   +1 more source

CCT2 Promotes Prostate Cancer Progression Through EIF3F‐Dependent Stabilization of FASN

Advanced Science, EarlyView.
ABSTRACT Prostate cancer (PCa) is increasingly recognized to be driven by dysregulated lipid metabolism. Although fatty acid synthase (FASN) is highly expressed in PCa, the mechanisms governing FASN protein stability and its functional integration into oncogenic lipid metabolism remain poorly defined.
Shun Xu, Yifan Zhang, Haolin Li, Shengyu Zhao, Xiaoran Dai, Qili Xu, Mintian Fei, Chun Li, Zhihui Zou, Baojun Wang, Li Zhang, Hui Wang, Ligang Zhang, Chaozhao Liang   +13 more
wiley   +1 more source

Sucrose as an electron source for cofactor regeneration in recombinant Escherichia coli expressing invertase and a Baeyer Villiger monooxygenase

Microbial Cell Factories
Background The large-scale biocatalytic application of oxidoreductases requires systems for a cost-effective and efficient regeneration of redox cofactors. These represent the major bottleneck for industrial bioproduction and an important cost factor. In
Lucija Sovic, Lenny Malihan-Yap, Gábor Szilveszter Tóth, Vilja Siitonen, Véronique Alphand, Yagut Allahverdiyeva, Robert Kourist   +6 more
doaj   +1 more source