Results 51 to 60 of about 131,570 (298)

Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3) [PDF]

Microbial Cell Factories, 2012
Abstract Background Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory system in Escherichia coli BL21(DE3), and it was found that ...
Su, Lingqia, Chen, Sheng, Yi, Li, Woodard, Ronald W, Chen, Jian, Wu, Jing   +5 more
openaire   +4 more sources

Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity

Communications Biology, 2021
Heyde and Nørholm investigate the role of RNAse E in the toxicity mediated by overexpression of heterologous proteins in derivatives of the BL21(DE3) strain of E. coli.
Sophia A. H. Heyde, Morten H. H. Nørholm   +1 more
doaj   +1 more source

PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP [PDF]

, 2009
This research was aimed at producing a crude intracellular phytase characterized from recombinant bacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+) +pEAS1 into competent E. coli BL21 and E.
Bachruddin, Z., Nuhriawangsa, A. M. P., SAJIDAN,, Wibowo, A.   +3 more
core  

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Biotechnology Reports, 2017
Rare arginine codons AGA and AGG affect the heterologous expression of proteins in Eschericha coli. The tRNAs necessary for protein synthesis are scarce in E. coli strain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus −RIL.
Beatriz Aguirre-López, Nallely Cabrera, Marietta Tuena de Gómez-Puyou, Ruy Perez-Montfort, Armando Gómez-Puyou   +4 more
doaj   +1 more source

Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate in E. coli K12 strain TG1

Microbial Cell Factories, 2021
Background Recently it was shown that production of recombinant proteins in E. coli BL21(DE3) using pET based expression vectors leads to metabolic stress comparable to a carbon overfeeding response. Opposite to original expectations generation of energy
Jan Weber, Zhaopeng Li, Ursula Rinas
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

A eubacterial origin for the human tRNA nucleotidyltransferase? [PDF]

, 2001
tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily.
Aebi M., Altschul S.F., Chen J.Y., Cudny H., Deutscher M.P., Deutscher M.P., Deutscher M.P., Deutscher M.P., Martin G., Raynal L.C., Saitou N., Yue D., Zhu L.   +12 more
core   +1 more source

Production of Indigo by Immobilization of E. coli BL21 (DE3) Cells in Calcium-Alginate Gel Capsules [PDF]

Chinese Journal of Chemical Engineering, 2007
Abstract The ability of catalyzing indole into indigo of gene engineering strain expressing P450 BM3 immobilized by entrapment in calcium-alginate gel capsules was examined, and various characteristics of immobilized cells were assessed. Optimum conditions for cells activity were not affected after immobilization, and pH and temperature for both free
Yan LU, Lehe MEI
openaire   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source