Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications [PDF]
, 2014 External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great
Bianchi, Dario Alejandro +2 more
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Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation [PDF]
Microbial Cell Factories, 2013 Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels.
Collins, Tony +5 more
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Acta Biochimica Indonesiana, 2019 Background: Immediate and accurate diagnosis of malaria is essential for effective control of this disease. Immunochromatographic based rapid diagnostic tests (RDTs) are economical, simple to perform, and provide results in a relative short time, can be
Fifi Fitriyah Masduki +6 more
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TRANSFORMASI PLASMID YANG MENGANDUNG GEN merB PADA Escherichia coli BL21(DE3) [PDF]
, 2019 DNA transformation is one of the methods for inserting DNA into bacterial cells. The current transformation method is widely used to transfer plasmids containing genetic material.
Bernadus, Zefanya G +2 more
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In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6 [PDF]
, 2009 Enzymes of the AlkB and CYP153 families catalyze the first step in the catabolism of medium-chain-length alkanes, selective oxidation of the alkane to the 1-alkanol, and enable their host organisms to utilize alkanes as carbon sources.
Arnold, Frances H. +3 more
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Microbial Cell Factories, 2022 Background Escherichia coli is one of the most important hosts for production of recombinant proteins in biopharmaceutical industry. However, when selecting a suitable production strain, it is often not considered that a lot of different sub-species ...
Florian Mayer +7 more
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Genetic incorporation of D-Lysine into diketoreductase in Escherichia coli cells [PDF]
, 2012 D-Lysine has been genetically introduced into diketoreductase in E. coli cells by utilization of an orthogonal Ph tRNA /Lysyl-tRNA synthetase pair.
Denghuan Yi +4 more
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β-Glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus [PDF]
, 2020 A clone encoding carboxymethyl cellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host.
Alessi, Anna M +11 more
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High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization [PDF]
, 2015 The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is ...
Fernández Lahore, Marcelo +5 more
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Engineering Escherichia coli BL21(DE3) Derivative Strains To Minimize E. coli Protein Contamination after Purification by Immobilized Metal Affinity Chromatography [PDF]
Applied and Environmental Microbiology, 2011 ABSTRACT Recombinant His-tagged proteins expressed in Escherichia coli and purified by immobilized metal affinity chromatography (IMAC) are commonly coeluted with native E. coli proteins, especially if the recombinant protein is expressed at a low level. The E. coli
Carine, Robichon +4 more
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