Different vectors used to transform and clone of nonstructural NS1 gene of Influenza B in Escherichia coli [PDF]
Iraqi Journal of Veterinary Sciences, 2019 Flu is a highly contagious and common illness caused by influenza A, B, and C viruses. The aim of the present study was to investigate the transformation and cloning of NS1B gene with pET-32a, pET-32b and pQE-81L in Escherichia coli BL21(DE3) and DH5α ...
Ali Dawood
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Characterisation of the bacterial biosensor GMG in E‐coli BL21 (DE3)
Engineering Biology, 2019 In this work, a gold biosensor has been constructed and characterised in Escherichia coli ( E. coli ). Though gold biosensors have been previously reported, a NOT gate gold biosensing genetic circuit was constructed using an orthogonal inverter system ...
RASHMI RAJASABHAI +2 more
openaire +2 more sources
Biotechnology for Biofuels, 2020 Background Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source.
Tong Wu +8 more
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Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone [PDF]
, 2013 The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of
Altschul +30 more
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Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability [PDF]
, 2006 Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9 ...
Erable, Benjamin +4 more
core +4 more sources
Effect of Desaturase Gene Overexpression on Fatty Acid Synthesis in Escherichia coli [PDF]
Shipin KexueIn order to obtain efficient engineered strains for oil production, the desaturase gene from Bacillus subtilis HB1310, an endophytic bacterium isolated from walnut, was expressed in Escherichia coli BL21 (DE3), the single-gene-expression strains BL21(DE3)
YE Jing, XU Siyuan, ZHANG Qin, QIAN Cheng, CAO Juanjuan, ZHAO Pei
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Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21(DE3) Leads to Increase of the Fatty Acid Biotransformation Activity. [PDF]
PLoS ONE, 2016 The biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. The productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance.
Ji-Min Woo +4 more
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Non-conventional gas phase remediation of volatile halogenated compounds by dehydrated bacteria [PDF]
, 2009 Traditional biological removal processes are limited by the low solubility of halogenated compounds in aqueous media. A new technology appears very suitable for the remediation of these volatile organic compounds (VOCs). Solid/gas bio-catalysis applied
Erable, Benjamin +3 more
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Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation [PDF]
, 2015 Figure S1. Physiology of recombinant E. coli BL21(DE3)(pLysS) strains bearing pET-24a. Panel A and B show biomass formation (circles), glucose consumption (squares), acetate formation (triangles), and proline consumption (diamonds) during batch ...
Andreas Schmid +3 more
core +3 more sources
Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis
Frontiers in Microbiology, 2019 Bacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation.
Ping Li +7 more
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