E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3) [PDF]
Microbial Cell Factories, 2018 Background Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is frequently employed due to its advantageous feature of lacking proteases which avoids degradation of target ...
Johanna Hausjell +4 more
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Reduction of the sulfoxide in glucoraphanin and sulforaphane by E. coli VL11 and BL21 (DE3) [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2016 Sulfoxide reductase activity of two Escherichia coli strains VL11 from human feces and BL21(DE3) from a molecular cloning host was expressed upon glucoraphanin induction for 16 hrs at 37°C under aerobic conditions.
Vijitra Luang-In +1 more
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Cascaded processing enables continuous upstream processing with E. coli BL21(DE3). [PDF]
Sci Rep, 2021 AbstractIn many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated.
Kittler S +8 more
europepmc +5 more sources
Expression and Functional Analysis of the Argonaute Protein of Thermus thermophilus (TtAgo) in E. coli BL21(DE3). [PDF]
Biomolecules, 2021 The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts. In the present study, we expressed functional Thermus thermophilus pAgo (TtAgo) in E. coli BL21 (DE3)
Xing J +7 more
europepmc +5 more sources
High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG. [PDF]
Microb Cell Fact, 2015 For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routinely cultured in Lysogeny broth (LB medium) and expression of the chromosomally localized t7rnap gene is governed by the isopropyl-β-D-1 ...
Zhang Z +6 more
europepmc +4 more sources
Generic Model Control Applied to E. coli BL21(DE3) Fed-Batch Cultures [PDF]
Processes, 2020 This work proposes a Generic Model Control (GMC) strategy to regulate biomass growth in fed-batch cultures of Escherichia coli BL21(DE3). The control law is established using a previously validated mechanistic model based on the overflow metabolism paradigm.
Merouane Abadli +4 more
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Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3) [PDF]
Genome Announcements, 2017 ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21 ...
Goffin, Philippe, Dehottay, Philippe
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Cloning and expression of native streptokinase in E. coli BL21(DE3)
International journal of health sciences, 2022 Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria. Streptokinase is one of the most important thrombolytic agents that was frequently employed treatment of myocardial infarction. In this study, we amplified the SK gene isolated from Streptococcus pyogenes. The sequence of SK was recorded in
Almuthana K. Hameed +2 more
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High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]
PLoS ONE, 2013 Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie +6 more
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Enhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters [PDF]
Journal of Sciences, Islamic Republic of Iran, 2018 Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector.
Z. Hajihassan, N. Biroonro
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