Results 11 to 20 of about 131,570 (298)
Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). [PDF]
PLoS ONE, 2011 The Escherichia coli B strain BL21(DE3) has had a profound impact on biotechnology through its use in the production of recombinant proteins. Little is understood, however, regarding the physiology of this important E. coli strain. We show here that BL21(
Constanze Pinske +4 more
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Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine [PDF]
Journal of Genetic Engineering and BiotechnologyThis study systematically evaluated Escherichia coli (E. coli) expression systems for the production of a humanized single-chain variable fragment targeting interleukin-17A (hScFv-IL-17A).
Weeraya Thongkum +6 more
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Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli [PDF]
Shipin Kexue, 2023 Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E.
LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
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Engineering Microbiology, 2022 Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotechnological production of proteins and biochemicals. In particular, the probiotic status of one E. coli strain, E.
Linlin Zhao +5 more
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A Dual-Bacterial Coupled Fermentation Strategy for Nicotinamide Mononucleotide Synthesis [PDF]
Shipin Kexue, 2023 In this study, a dual-bacterial coupled fermentation system containing nicotinamide nucleoside kinase (NRK) and polyphosphatase (PPK) was constructed, and the application of PPK-based ATP regeneration system in NMN production was achieved.
SUN Ting, ZHANG Hongtao, YANG Feng, CHAI Wengang, XUE Haoyang, TAN Shuyin
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Revista Colombiana de Biotecnología, 2013 Título en ingles: Expression and purification of rotavirus structural proteins VP5* and VP8* in bacteria E. coli BL21(DE3) Resumen La caracterización de las proteínas estructurales del rotavirus y de las proteínas de la superficie de la célula hospedera ...
Luz Yurany Moreno +2 more
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Generic Model Control Applied to E. coli BL21(DE3) Fed-Batch Cultures [PDF]
Processes, 2020 This work proposes a Generic Model Control (GMC) strategy to regulate biomass growth in fed-batch cultures of Escherichia coli BL21(DE3). The control law is established using a previously validated mechanistic model based on the overflow metabolism paradigm.
Merouane Abadli +4 more
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Interleukin gene cloning and expression in E. coli
Annals of Medicine, 2021 Introduction Cytokines are a large category of protins; they play various functions from inflammation response to cancer development and sepsis. Over-expression or problems in production control of this small peptides can lead to the development of ...
Filipa Oliveira, Fernando Cardoso
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Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3) [PDF]
Genome Announcements, 2017 ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21 ...
Goffin, Philippe, Dehottay, Philippe
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Engineering in Life Sciences, 2023 γ‐Aminobutyric acid (GABA) is a kind of non‐proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries.
Liu Fengmin +5 more
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