Results 1 to 10 of about 92,652 (117)
Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production [PDF]
Microbial Cell Factories, 2021 Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (PlacUV5 ...
Fei Du +6 more
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Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation [PDF]
BMC Microbiology, 2021 Background Microorganisms can prioritize the uptake of different sugars depending on their metabolic needs and preferences. When both D-glucose and D-xylose are present in growth media, E.
Jung Min Heo, Hyun Ju Kim, Sang Jun Lee
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Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. [PDF]
PLoS ONE, 2014 Excess acetate has long been an issue for the production of recombinant proteins in E. coli cells. Recently, improvements in acetate tolerance have been achieved through the use of genetic strategies and medium supplementation with certain amino acids ...
Hengwei Wang +6 more
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Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli [PDF]
Shipin Kexue, 2023 Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E.
LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
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Engineering Microbiology, 2022 Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotechnological production of proteins and biochemicals. In particular, the probiotic status of one E. coli strain, E.
Linlin Zhao +5 more
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A Dual-Bacterial Coupled Fermentation Strategy for Nicotinamide Mononucleotide Synthesis [PDF]
Shipin Kexue, 2023 In this study, a dual-bacterial coupled fermentation system containing nicotinamide nucleoside kinase (NRK) and polyphosphatase (PPK) was constructed, and the application of PPK-based ATP regeneration system in NMN production was achieved.
SUN Ting, ZHANG Hongtao, YANG Feng, CHAI Wengang, XUE Haoyang, TAN Shuyin
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Interleukin gene cloning and expression in E. coli
Annals of Medicine, 2021 Introduction Cytokines are a large category of protins; they play various functions from inflammation response to cancer development and sepsis. Over-expression or problems in production control of this small peptides can lead to the development of ...
Filipa Oliveira, Fernando Cardoso
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Engineering in Life Sciences, 2023 γ‐Aminobutyric acid (GABA) is a kind of non‐proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries.
Liu Fengmin +5 more
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Revista Colombiana de Biotecnología, 2020 Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle +2 more
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Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3). [PDF]
PLoS ONE, 2018 Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins.
Hanseol Kim, Sinyeon Kim, Sung Ho Yoon
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