Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation [PDF]
BMC Microbiology, 2021 Background Microorganisms can prioritize the uptake of different sugars depending on their metabolic needs and preferences. When both D-glucose and D-xylose are present in growth media, E.
Jung Min Heo, Hyun Ju Kim, Sang Jun Lee
doaj +5 more sources
Cascaded processing enables continuous upstream processing with E. coli BL21(DE3). [PDF]
Sci Rep, 2021 AbstractIn many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated.
Kittler S +8 more
europepmc +5 more sources
PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP [PDF]
Journal of the Indonesian Tropical Animal Agriculture, 2014 This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E.
A.M.P. Nuhriawangsa +3 more
doaj +2 more sources
Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production [PDF]
Microbial Cell Factories, 2021 Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (PlacUV5 ...
Fei Du +6 more
doaj +2 more sources
E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3) [PDF]
Microbial Cell Factories, 2018 Background Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is frequently employed due to its advantageous feature of lacking proteases which avoids degradation of target ...
Johanna Hausjell +4 more
doaj +4 more sources
Expression and Functional Analysis of the Argonaute Protein of Thermus thermophilus (TtAgo) in E. coli BL21(DE3). [PDF]
Biomolecules, 2021 The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts. In the present study, we expressed functional Thermus thermophilus pAgo (TtAgo) in E. coli BL21 (DE3)
Xing J +7 more
europepmc +5 more sources
High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG. [PDF]
Microb Cell Fact, 2015 For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with T7-promoter based expression vectors is widely used. Cells are routinely cultured in Lysogeny broth (LB medium) and expression of the chromosomally localized t7rnap gene is governed by the isopropyl-β-D-1 ...
Zhang Z +6 more
europepmc +4 more sources
Reduction of the sulfoxide in glucoraphanin and sulforaphane by E. coli VL11 and BL21 (DE3) [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2016 Sulfoxide reductase activity of two Escherichia coli strains VL11 from human feces and BL21(DE3) from a molecular cloning host was expressed upon glucoraphanin induction for 16 hrs at 37°C under aerobic conditions.
Vijitra Luang-In +1 more
doaj +2 more sources
Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. [PDF]
PLoS ONE, 2014 Excess acetate has long been an issue for the production of recombinant proteins in E. coli cells. Recently, improvements in acetate tolerance have been achieved through the use of genetic strategies and medium supplementation with certain amino acids ...
Hengwei Wang +6 more
doaj +2 more sources
BiomedicinesThe CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, while
Shilpi Agrawal +6 more
doaj +3 more sources