Results 21 to 30 of about 92,801 (255)
PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP
Journal of the Indonesian Tropical Animal Agriculture, 2014 This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E.
A.M.P. Nuhriawangsa +3 more
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Engineered Escherichia coli strains as platforms for biological production of isoprene
FEBS Open Bio, 2020 Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21 ...
Hyeok‐Won Lee +7 more
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Onderstepoort Journal of Veterinary Research, 2016 Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C.
Fatemah Bakhshi +2 more
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Data, 2021 The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the ...
Rafida Razali +2 more
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Characterisation of the bacterial biosensor GMG in E‐coli BL21 (DE3)
Engineering Biology, 2019 In this work, a gold biosensor has been constructed and characterised in Escherichia coli ( E. coli ). Though gold biosensors have been previously reported, a NOT gate gold biosensing genetic circuit was constructed using an orthogonal inverter system ...
RASHMI RAJASABHAI +2 more
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Different vectors used to transform and clone of nonstructural NS1 gene of Influenza B in Escherichia coli [PDF]
Iraqi Journal of Veterinary Sciences, 2019 Flu is a highly contagious and common illness caused by influenza A, B, and C viruses. The aim of the present study was to investigate the transformation and cloning of NS1B gene with pET-32a, pET-32b and pQE-81L in Escherichia coli BL21(DE3) and DH5α ...
Ali Dawood
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Biotechnology for Biofuels, 2020 Background Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source.
Tong Wu +8 more
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Effect of Desaturase Gene Overexpression on Fatty Acid Synthesis in Escherichia coli [PDF]
Shipin KexueIn order to obtain efficient engineered strains for oil production, the desaturase gene from Bacillus subtilis HB1310, an endophytic bacterium isolated from walnut, was expressed in Escherichia coli BL21 (DE3), the single-gene-expression strains BL21(DE3)
YE Jing, XU Siyuan, ZHANG Qin, QIAN Cheng, CAO Juanjuan, ZHAO Pei
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Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21(DE3) Leads to Increase of the Fatty Acid Biotransformation Activity. [PDF]
PLoS ONE, 2016 The biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. The productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance.
Ji-Min Woo +4 more
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Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation [PDF]
Microbial Cell Factories, 2013 Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels.
Collins, Tony +5 more
openaire +3 more sources