Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle +2 more
doaj +1 more source
Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3). [PDF]
Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins.
Hanseol Kim, Sinyeon Kim, Sung Ho Yoon
doaj +1 more source
Cloning and expression of native streptokinase in E. coli BL21(DE3)
Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria. Streptokinase is one of the most important thrombolytic agents that was frequently employed treatment of myocardial infarction. In this study, we amplified the SK gene isolated from Streptococcus pyogenes. The sequence of SK was recorded in
Almuthana K. Hameed +2 more
openaire +1 more source
High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]
Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie +6 more
doaj +1 more source
Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3) [PDF]
Membrane protein production is usually toxic to E. coli. However, using genetic screens strains can be isolated in which the toxicity of membrane protein production is reduced, thereby improving production yields. Best known examples are the C41(DE3) and
Baumgarten, Thomas +10 more
core +9 more sources
Enhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters [PDF]
Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector.
Z. Hajihassan, N. Biroonro
doaj +1 more source
Engineered Escherichia coli strains as platforms for biological production of isoprene
Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21 ...
Hyeok‐Won Lee +7 more
doaj +1 more source
The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the ...
Rafida Razali +2 more
doaj +1 more source
Microbial Cell Factories / Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production [PDF]
Background: In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration ...
Bayer, Karl +4 more
core +2 more sources
Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C.
Fatemah Bakhshi +2 more
doaj +1 more source

