Results 111 to 120 of about 131,570 (298)

Nanoscale Spatial Organization of ARC High‐ and Low‐Order Assemblies at Excitatory Synapses

Advanced Science, EarlyView.
ARC (Activity‐Regulated Cytoskeleton‐Associated protein) mediates synaptic plasticity by forming nanoscale assemblies in neurons. Using super‐resolution microscopy and time‐resolved anisotropy with targeted tagging, the study reveals low‐order ARC assemblies at synapses colocalizing with AMPARs, semi‐circular structures at endocytic zones, and 60–80 nm
Martina Damenti, Giovanna Coceano, Mariline Mendes Silva, Jonatan Alvelid, Chiara Sgattoni, Andrea Volpato, Lea Rems, Luciano A. Masullo, Eduard M. Unterauer, Rafal Kowalewski, Lucie Delemotte, Erdinc Sezgin, Ralf Jungmann, Ilaria Testa   +13 more
wiley   +1 more source

Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli

Hayati Journal of Biosciences, 2016
Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available.
Hamdayanty Hamdayanty, Sri Hendrastuti Hidayat, Tri Asmira Damayanti   +2 more
doaj   +1 more source

Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs [PDF]

, 2014
Background: Lipases including the lipase from Burkholderia cepacia are in a main focus in biotechnology research since many years because of their manifold possibilities for application in industrial processes.
Detzel, C. (Christian), Jose, J. (Joachim), Kranen, E.E. (Eva), Weber, T. (Thomas)   +3 more
core   +1 more source

ZSL Orchestrates Synaptonemal Complex Assembly as a Central Region Scaffold to Ensure Synapsis Fidelity and Crossover Control in Polyploid Meiosis

Advanced Science, EarlyView.
A central molecular adaptor, ZSL, is identified that links transverse filaments ZYP1 to central element SCEP1/2 to drive synaptonemal complex assembly in Brassica napus. Loss of ZSL abolishes synaptonemal complex formation, disrupts meiotic chromosome segregation, and markedly increases crossovers, providing mechanistic insight into meiotic fidelity ...
Miaowei Geng, Shaochen Jia, Fei Cao, Cuiping Liu, Heshuang Zhang, Gang Xu, Jixin Zhuang, Yashi Zhang, Can Liu, Yuhang Zhao, Zilin Guo, Xinjie Yuan, Jiaqing Yang, Lei Chu, Bowei Cai, Hu Zhao, Chao Yang   +16 more
wiley   +1 more source

Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes [PDF]

, 2008
RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of
Malathi Raghunathan, Shilpakala Sainath Rao   +1 more
core   +1 more source

Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli [PDF]

, 2016
The expansion of microbial substrate and product scopes will be an important brick promoting future bioeconomy. In this study, an orthogonal pathway running in parallel to native metabolism and converting renewable dodecanoic acid methyl ester (DAME) via
Aßmann, M, Bühler, B, Julsing, MK, Ladkau, N, Schmid, A, Schrewe, M   +5 more
core   +1 more source

An ATP‐Mediated Antibiotic β‐Peptide Nanofiber That Kills Multidrug‐Resistant Bacteria via a Multistage Mechanism

Advanced Science, EarlyView.
The 3K‐AP system forms infinite supramolecular fibrils that undergo controlled degradation into shorter fragments upon enzymatic hydrolysis. Multitechnique imaging reveals a stepwise bacteriolytic mechanism involving bacterial entrapment, disassembly into lethal constructs, and associated extracellular vesicle release, highlighting the dynamic ...
Sohini Chakraborty, Kamal el Battioui, Dániel Molnár, Bálint Jezsó, Lejla Daruka, Tasvilla Sonallya, Tünde Juhász, Rita Hirmondó, Imola Cs. Szigyártó, Loránd Románszki, Natália Tőkési, Daniel Pinkas, Eszter Házy, Olivér Pavela, Andrea Bodor, Zoltán Varga, István Mándity, Mihály Kovács, Csaba Pál, Kata Horváti, Judit Tóth, Tamás Beke‐Somfai   +21 more
wiley   +1 more source

Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins [PDF]

, 2015
Es va publicar un treball amb esmenes a aquest article que es pot consultar a https://ddd.uab.cat/record/185349 (DOI 10.1186/s12934-015-0265-x)Background: lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer ...
Bramhill, David, Corchero Nieto, José Luis, Head, Steven Robert, Kohl, Thomas Andreas, Lindner, Buko, Mamat, Uwe, Meredith, Timothy Charles, Schaffer, Lana, Schromm, Andra Beate, Souvignier, Chad, Villaverde Corrales, Antonio, Wilke, Kathleen, Woodard, Ronald Wesley   +12 more
core   +1 more source

Cloning and Expression of C2 and V Domains of ALCAM Protein in E. coli BL21 (DE3)

Clinical Microbiology: Open Access, 2017
Introduction: ALCAM as a glycoprotein is a member of the immunoglobulin family and plays an important role in cell growth, survival and motility. This protein also contributes to the development of tumour invasion in colorectal and breast cancers and is typically considered as a cancer stem cell marker.
Hassan Dana, Ali Mazraeh, Ghanbar Mahmoodi Chalbatani, Vahid Marmari, Habibollah Mahmoodzadeh, Ali Ghamari, Fateme Moazzen, Mohammad Ebrahimi, Narges Mehmandoost   +8 more
openaire   +1 more source

POU2F1 Promotes Chemoresistance in Colorectal Cancer Cells via Attenuates the MDR2 Degradation Mediated by PPP1R11 Lactylation

Advanced Science, EarlyView.
Schematic diagram of our study showing that highly expressed POU2F1 in colorectal cancer (CRC) can directly accelerate the cytosolic lactate export to decrease PPP1R11 lactylation at K59, thereby attenuating the E3 ligase enzyme activity and protein stability of PPP1R11, which inhibits the ubiquitination of MDR2 at K413 and K538 but increases the ...
Longzheng Xia, Jinguan Lin, Xianjie Jiang, Linda Oyang, Shiming Tan, Zongyao Ren, Qiu Peng, Qianjin Liao, Yujuan Zhou   +8 more
wiley   +1 more source