Results 191 to 200 of about 1,499,816 (300)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effective Sample Size: Quick Estimation of the Effect of Related Samples in Genetic Case-Control Association Analyses

, 2007
Correlated samples have been frequently avoided in case-control genetic association studies in part because the methods for handling them are either not easily implemented or not widely known.
Daniel Kastner, Peter K. Gregersen, Wentian F. Li, Yaning B. Yang, Chukwuma Ogunwole, Elaine L. Remmers   +5 more
core  

Estimating Recent and Historical Effective Population Size of Marine and Freshwater Sticklebacks. [PDF]

Mol Ecol
Feng X, Löytynoja A, Merilä J.
europepmc   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Estimating the Effective Population Size Across Space and Time in the Critically Endangered Western Chimpanzee in Guinea-Bissau: Challenges and Implications for Conservation Management. [PDF]

Evol Appl
da Silva MJF, Borges F, Gerini F, Sá RM, Silva F, Maié T, Hernández-Alonso G, Ramos-Madrigal J, Gopalakrishnan S, Aleixo-Pais I, Djaló SL, Fernandes N, Camará I, Regalla A, Casanova C, Costa M, Colmonero-Costeira I, Rodríguez Fernandes C, Chikhi L, Minhós T, Bruford MW.   +20 more
europepmc   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Dealing With the Complexity of Effective Population Size in Conservation Practice. [PDF]

Evol Appl
Fedorca A, Mergeay J, Akinyele AO, Albayrak T, Biebach I, Brambilla A, Burger PA, Buzan E, Curik I, Gargiulo R, Godoy JA, González-Martínez SC, Grossen C, Heuertz M, Hoban S, Howard-McCombe J, Kachamakova M, Klinga P, Köppä V, Neugebauer E, Paz-Vinas I, Pearman PB, Pérez-Sorribes L, Rinkevich B, Russo IM, Theraroz A, Thomas NE, Westergren M, Winter S, Laikre L, Kopatz A.   +30 more
europepmc   +1 more source

Effective population size in a partially clonal plant is not predicted by the number of genetic individuals. [PDF]

Evol Appl, 2023
Gargiulo R, Waples RS, Grow AK, Shefferson RP, Viruel J, Fay MF, Kull T.   +6 more
europepmc   +1 more source

Estimating Effective Population Size: A Reply to Nunney [PDF]

Evolution, 1995
Brian C, Husband, Spencer C H, Barrett
openaire   +2 more sources