Results 121 to 130 of about 226,631 (305)

Needle‐free, Liquid Metal‐embedded Electrospray Deposition System for Controlled Microdroplet Printing

Advanced Science, EarlyView.
The liquid metal‐embedded electrospray deposition (LM‐ESD) system combines stable droplet spray, clog‐free and recoverable operation, and simplified fabrication in a single polydimethylsiloxane (PDMS) microfluidic platform. By eliminating external electrodes, it offers needle‐free, robust, and scalable droplet array printing, ensuring reproducibility ...
Chaeyeong Kang, Seokbeom Roh, Hyunjun Park, Sugeun Lee, Taeha Lee, Sang Won Lee, Hansung Kim, Jinsung Park, Gyudo Lee, Insu Park   +9 more
wiley   +1 more source

Aloe‐Emodin Targeting FOXC2 Disrupts NETs Formation and EMT‐Driven Postoperative Peritoneal Adhesion Through TGF‐β1‐Smad2/3 Pathway

Advanced Science, EarlyView.
Forkhead box protein C2 (FOXC2) is highlighted as a pivotal effector in postoperative peritoneal adhesion (PPA) formation. Mechanically, FOXC2 mediates NETs‐induced EMT alterations through the TGF‐β1‐Smad2/3 pathway. Aloe‐emodin (AE) ameliorates FOXC2‐driven fibrosis by blocking NETs formation and EMT changes through this pathway.
Lili Yang, Yunda Fang, Yuheng Lian, Ziyang Kong, Jia Miao, Yanqi Chen, Wen Li, Feiyan Chen, Bin Zhang, Yao Chen, Yaoyao Bian   +10 more
wiley   +1 more source

Inverse Shifting-PCR Modified by Capillary Electrophoresis for Detecting F8 int22h and int1h Inversions in Severe Hemophilia A Patients and Probable Carriers

Life
Globally, intron 22 inversions (Inv22s) of the factor VIII gene (F8) are the most frequent pathogenic variants and account for 45–50% of severe hemophilia A (SHA) cases, while intron 1 inversion (Inv1) explains 1–5% of SHA cases.
Rosa Michel Martínez-Contreras, Silvia Sofía García-López, Hilda Luna-Záizar, Ana Rebeca Jaloma-Cruz   +3 more
doaj   +1 more source

Characterization of femtosecond laser written waveguides for integrated biochemical sensing [PDF]

, 2007
Fluorescence detection is known to be one of the most sensitive among the different optical sensing techniques. This work focuses on excitation and detection of fluorescence emitted by DNA strands labeled with fluorescent dye molecules that can be ...
Cerullo, G., Dekker, R., Dongre, C., Hoekstra, H.J.W.M., Laporta, P., Martinez-Vazquez, R., Nolli, D., Osellame, R., Pollnau, M.   +8 more
core   +1 more source

Pulmonary‐Targeted Nanoparticles Interrupt the Malignant Mechanical and Biochemical Signaling Crosstalk for Idiopathic Pulmonary Fibrosis Therapy

Advanced Science, EarlyView.
Idiopathic pulmonary fibrosis (IPF) progression is driven by TGF‐β induced biochemical signals and mechanical signals from increased ECM stiffness. Pulmonary‐targeted VB‐RT NPs not only eliminate excessive ROS and penetrate the dense ECM collagen barrier via NO release, but also simultaneously interrupt the persistent malignant between mechanical and ...
Xue‐Na Li, Ya‐Ping Lin, Xi‐Xi Ma, Yue‐Fei Fang, Hui Wang, Chun‐Hui Cui, Chen Zhang, Jin‐You Piao, Jee‐Heon Jeong, Xian Wu Cheng, Lei Xing, Hu‐Lin Jiang   +11 more
wiley   +1 more source

Artifacts of analysis in cell line identification by short tandem repeat profiling

Кардиоваскулярная терапия и профилактика
Aim. To study and describe the most common types of artifacts in detection of short tandem repeat (STR) amplicons by capillary electrophoresis and cause difficulties in interpreting the obtained STR profiles.Material and methods. Cell lines were obtained
A. A. Malchenkova, E. N. Kosobokova
doaj   +1 more source

Genome‐scale Metabolic Modeling Guided Escherichia coli Engineering for De Novo Biosynthesis of Chrysanthemic Acid

Advanced Science, EarlyView.
Genome‐scale metabolic modeling is preceded to the engineered E. coli cell factory for the de novo biosynthesis of biopesticide chrysanthemic acid. The debranching strategy redirects metabolic flux, enhancing precursor and target compound production.
Jiangpeng Yu, Kelin Cheng, Shenyang Qu, Jie Wang, Xun Wang, Cheng Zhao, Wei Li   +6 more
wiley   +1 more source

ARL5B Drives Esophageal Squamous Cell Carcinoma Progression via ROCK1–SREBP1‐Mediated Lipid Metabolic Reprogramming

Advanced Science, EarlyView.
Esophageal squamous cell carcinoma urgently requires new therapeutic strategies. Adenosine diphosphate‐ribosylation factor‐like protein 5B (ARL5B) is identified as a novel oncogene that remodels fatty acid metabolism through the ras homologous‐associated coiled‐coil containing protein kinase 1 (ROCK1)–sterol regulatory element‐binding protein 1 (SREBP1)
Xinyue Ma, Yanfei Sun, Hongyuan Mao, Chenhan Huang, Zerun Li, Tianzi Wang, Dizhi Jiang, Xinyu Zhang, Zhenyu Yuan, Zhihui Zhang, Bo Cheng, Ruiqing Wang, Yufeng Cheng   +12 more
wiley   +1 more source

A Dynamic Gate Enables Regioselective Hydroxylation of Free Arginine by a Non‐Canonical Heme Enzyme

Advanced Science, EarlyView.
The YqcI/YcgG enzyme AglA employs a unique “sandwich” fold and a dynamic gating mechanism to selectively hydroxylate AMPn‐Arg at Nε. Site‐directed mutations reprogram regioselectivity or enable activity on free arginine by altering hydrogen‐bond networks, precisely positioning substrates.
Yuan Sun, Chao Dou, Weizhu Yan, Pengpeng Chen, Lu Zhang, Dan Zhou, Yanhui Zheng, Zhaolin Long, Shoujie Li, Xiaoqing Xu, Qiuxia Huang, Xiaofeng Zhu, Wei Cheng   +12 more
wiley   +1 more source

Sample dispersion in isotachophoresis with Poiseuille counterflow

, 2013
A particular mode of isotachophoresis (ITP) employs a pressure-driven flow opposite to the sample electromigration direction in order to anchor a sample zone at a specific position along a channel or capillary.
Baier, Tobias, Bhattacharyya, Somnath, Gopmandal, Partha P., Hardt, Steffen   +3 more
core   +1 more source