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Two-dimensional gel electrophoresis of membrane proteins
Biochemistry, 1976A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Sample preparation for two‐dimensional gel electrophoresis
PROTEOMICS, 2003Abstract The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two‐dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent ...
Margaret M, Shaw, Beat M, Riederer
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6 Two-dimensional gel electrophoresis
2005Summary 2-DE continues to set the benchmark as the premier technique for protein separation in protoemic applications. The approach is very well sited for routine separation of moderately abundant proteins within a window of pH 3-10 and M r =10-200 kDa, allowing for cataloging and quantitative measures of protein expression.
Mark P. Molloy, Michael T. McDowell
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Two-Dimensional Gel Electrophoresis: A Reference Protocol
2017Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s. Over this time, it has been used in the study of many diseases including cancer, diabetes, heart disease, and psychiatric disorders ...
Veronica M, Saia-Cereda +3 more
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Two-dimensional Gel Electrophoresis (2DE)
2013The chemical compounds, which are present in the environment, increasingly cause bad effects on health. The most serious effects are tumors and various mutations at the cellular level. Such compounds, from the analytical point of view, can serve the function of biomarkers, constituting measurable changes in the organism’s cells and biochemical ...
Ewa Kłodzińska, Bogusław Buszewski
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Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics
2016Phosphorylation is one of the most important reversible protein modifications and is involved in regulating signal transduction, subcellular localization and enzyme activity of target proteins. Phosphorylation or dephosphorylation of proteins is directly reflected in changed ratios of phosphoprotein abundance and total protein abundance.
Chao, Han, Pingfang, Yang
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Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
2012Two-dimensional gel electrophoresis (2-DE) is one of the most powerful tools for separating proteins based on their size and charge. 2-DE is very useful to separate two proteins with identical molecular weights but different charges, which cannot be achieved with just sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Here, a simpler
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Vapour-phase deposition of two-dimensional layered chalcogenides
Nature Reviews Materials, 2023Jiangtao Wang, Peng Wu, Jing Kong
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Low-dimensional wide-bandgap semiconductors for UV photodetectors
Nature Reviews Materials, 2023Ziqing Li, Xiaosheng Fang
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