Results 61 to 70 of about 854,016 (247)

Serum proteins and their diagnostic utility in veterinary medicine: a review

Veterinární Medicína, 2016
The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of ...
C. Tothova, O. Nagy, G. Kovac
doaj   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Fractionnement et caractérisation des glycoprotéines dans les moûts de raisins blancs

OENO One, 1990
Les auteurs étudient les glycoprotéines dans des moûts de raisins blancs. Ces glycoprotéines sont fractionnées par chromatofocalisation et caractérisées par différentes techniques séparatives : CLHP, électrophorèse, SDS-PAGE, analyse des acides aminés ...
Michael Paetzold, Laurent Dulau, Denis Dubourdieu   +2 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Gel Electrophoresis

Clinical Chemistry, 1962
Abstract Gel electrophoresis is discussed and it is shown that this medium is still in the developmental stage for the resolution of serum proteins. It is the procedure of choice for the analysis of hemoglobins.
S, RAYMOND, M, NAKAMICHI
openaire   +2 more sources

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Coupling gel electrophoresis with photoluminescence reveals biochrome complexes in modern and fossil shells

Methods in Ecology and Evolution
Polyacrylamide gel electrophoresis is commonly used to visualize mixtures of proteins, such as those embedded in calcareous biominerals. However, it is ineffective for the detection of biochromes, a major class of low molecular weight organic compounds ...
Luca Polacchi, Camille Lutet‐Toti, Olivier Basuyaux, Pierre Gueriau, Marie Albéric, Hélène Pasco, Jérôme Thomas, Didier Merle, Benoît Habermeyer, Roger Guilard, Mathieu Thoury, Frédéric Marin   +11 more
doaj   +1 more source

Genetic diversity of kaffir sorghum accessions from the VIR collection in kafirin-encoding loci

Труды по прикладной ботанике, генетике и селекции
Background. Sorghum is a universal, highly drought-resistant crop, widely cultivated throughout the world. Sorghum grain has a valuable biochemical composition and is utilized in both food and feed production.
N. V. Alpatieva, I. N. Anisimova, M. K. Ryazanova, V. V. Vasipov, R. A. Abdullaev, O. I. Romanova, E. E. Radchenko   +6 more
doaj   +1 more source

Fluid Dynamics and Electrical Detection of λDNA in Electrode-Embedded Nanochannels

Journal of Biomechanical Science and Engineering, 2013
In the present study, we address theoretical approaches for the experimental results to investigate the flow dynamics of λDNA through a nanochannel in which two nanoelectrodes are integrated.
Satoshi UEHARA, Makusu TSUTSUI, Kentaro DOI, Masateru TANIGUCHI, Satoyuki KAWANO, Tomoji KAWAI   +5 more
doaj   +1 more source