Results 201 to 210 of about 135,255 (300)

Approaches to Superefficient Yeast Transformations: DNA Phase Separation, Cell Growth State and Systematic Experimental Design

Yeast, EarlyView.
Yeast transformations are mechanistically poorly understood and could be made orders of magnitude more efficient by three approaches. (1) Investigate mechanisms of DNA uptake including DNA phase separation. (2) Account for growth state. (3) Use systematic statistical design of experiments to test combinations of factors that enhance transformation ...
Alistair Elfick, Edward W. J. Wallace
wiley   +1 more source

A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis

Yeast, EarlyView.
ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley   +1 more source

Progenitor Timing Shapes NG2-Glia Fate and Oligodendrocyte Differentiation. [PDF]

Glia
Ojalvo-Sanz AC, Pernia-Solanilla C, López-Mascaraque L.   +2 more
europepmc   +1 more source

Integrating Chemical, Electrical, and Photonic Effects Into a Unified ETOP‐Based Plasma Dose Framework

High Voltage, EarlyView.
ABSTRACT In plasma medicine and biological applications, plasmas typically deliver a complex combination of reactive chemical species and multiple physical energy components. However, establishing a unified and comparable dose metric to characterise their integrated biological effects remains a major challenge that continues to limit progress in this ...
Erqi Wu, Jialin Liu, Zhiyu Li, Dawei Liu, Lanlan Nie, Xinpei Lu   +5 more
wiley   +1 more source

From Lipid Composition to Electroporation: Mechanical Encoding of Barrier Resilience in Stratum Corneum Membranes. [PDF]

ACS Omega
Machado NCF, García NA, Anguita-Ortiz N, Ruano L, Nogueira JJ, Martinho HDS.   +5 more
europepmc   +1 more source

Mapping the lineage of prostate tissue ablation medical device predicates for focal therapy of prostate cancer approved via the FDA 510(k) clearance pathway

Surgical Practice, EarlyView.
Abstract Objective Medical devices are reviewed by the US Food and Drug Administration (FDA) for safety and effectiveness. Class II devices can receive 510(k) clearance if they are “substantially equivalent” to an existing predicate device. This process can be repeated iteratively, leading to a lineage of predicates for newly cleared devices.
Yiting Lin, Amir Khan, Arvin George, Mohummad Minhaj Siddiqui   +3 more
wiley   +1 more source

Biomaterial-Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation. [PDF]

Small
Ji B, Wu Z, Jiang Y, Liu X, Li J, Xu F, Li C, Shan Q, Wang Z, Wang J, Huang W, Zhuang Y, Cui W, Wang Z.   +13 more
europepmc   +1 more source

Interventional oncology in children: Where are we now?

Journal of Medical Imaging and Radiation Oncology, EarlyView.
Abstract Paediatric Interventional Oncology (IO) lags behind adult IO due to a scarcity of specific outcome data. The suboptimal way to evolve this field is relying heavily on adult experiences. The distinct tumour types prevalent in children, such as extracranial germ cell tumours, sarcomas, and neuroblastoma, differ strongly from those found in ...
Premal Amrishkumar Patel, Fernando Gómez Muñoz   +1 more
wiley   +1 more source

An integrated nanopore-microfluidic platform for low-voltage electroporation of delivering self-amplifying RNA into dendritic cells. [PDF]

Microsyst Nanoeng
Zhang B, Cai Y, Deng C, Huang X, Yao C, Li X, Gao L, Wu Y, Chen J, Jiang J, Shang L, Xie X, Wang J, Chen HJ, Wu Y, Liu J.   +15 more
europepmc   +1 more source

Inhibition of HMGA2 Leads to Reduced Cell Proliferation and Increased Apoptosis in Human Embryonal Carcinoma Cell Lines

Andrology, EarlyView.
ABSTRACT Background The most prevalent solid tumors in young men are testicular germ cell tumors (TGCTs), and embryonal carcinoma is the most common subtype among non‐seminomatous germ cell tumors (NSGCTs). Despite the excellent cure rates of cisplatin‐based chemotherapy, resistance develops in 15%–30% of patients with metastatic cancer, which results ...
Marco De Martino, Matteo Lampitto, Alfredo Fusco, Marco Barchi, Francesco Esposito, Paolo Chieffi   +5 more
wiley   +1 more source