Results 31 to 40 of about 206,297 (286)

The meganuclease I-SceI containing nuclear localization signal (NLS-I-SceI) efficiently mediated mammalian germline transgenesis via embryo cytoplasmic microinjection. [PDF]

PLoS ONE, 2014
The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition ...
Yong Wang, Xiao-Yang Zhou, Peng-Ying Xiang, Lu-Lu Wang, Huan Tang, Fei Xie, Liang Li, Hong Wei   +7 more
doaj   +1 more source

Editing DNA Methylation in Mammalian Embryos [PDF]

International Journal of Molecular Sciences, 2020
DNA methylation in mammals is essential for numerous biological functions, such as ensuring chromosomal stability, genomic imprinting, and X-chromosome inactivation through transcriptional regulation. Gene knockout of DNA methyltransferases and demethylation enzymes has made significant contributions to analyzing the functions of DNA methylation in ...
Taiga Yamazaki, Yu Hatano, Ryoya Taniguchi, Noritada Kobayashi, Kazuo Yamagata   +4 more
openaire   +2 more sources

The p66(Shc) adaptor protein controls oxidative stress response in early bovine embryos. [PDF]

PLoS ONE, 2014
The in vitro production of mammalian embryos suffers from high frequencies of developmental failure due to excessive levels of permanent embryo arrest and apoptosis caused by oxidative stress.
Dean H Betts, Nathan T Bain, Pavneesh Madan   +2 more
doaj   +1 more source

Modeling mammalian gastrulation with embryonic stem cells

, 2017
Understanding cell fate patterning and morphogenesis in the mammalian embryo remains a formidable challenge. Recently, in vivo models based on embryonic stem cells (ESCs) have emerged as complementary methods to quantitatively dissect the physical and ...
Amita, Arnold, Azioune, Bauwens, Bedzhov, Ben-Haim, Bernardo, Blin, Bolouri, Chambers, Coucouvanis, Dunn, Engler, Etoc, Farin, Gjorevski, Green, Gurdon, Harrison, Heemskerk, Heemskerk, Horii, Lee, Lei, Li, Lippmann, Loh, Meinhardt, Meinhardt, Mendjan, Migeotte, Müller, Nakamura, Nallet-Staub, Nemashkalo, Nemashkalo, Nowotschin, Pagliuca, Peerani, Perea-Gomez, Przybyla, Qin, Ranga, Rosowski, Saka, Sasai, Schohl, Shao, Shao, Simunovic, Sorre, Sun, Tang, ten Berge, Teo, Tewary, Théry, Turing, Turner, Turner, van den Brink, Warmflash, Wilson, Xu, Yang, Yu   +65 more
core   +1 more source

Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia. [PDF]

, 2018
Cell division is essential to expand, shape, and replenish epithelia. In the adult small intestine, cells from a common progenitor intermix with other lineages, whereas cell progeny in many other epithelia form contiguous patches.
Castillo-Azofeifa, David, Delling, Markus, Klein, Ophir D, McKinley, Kara L, Royer, Loic A, Schartner, Christoph, Stuurman, Nico, Vale, Ronald D   +7 more
core   +1 more source

Reorganization, specialization, and degradation of oocyte maternal components for early development

Reproductive Medicine and Biology, 2023
Background Oocyte components are maternally provided, solely determine oocyte quality, and coordinately determine embryo quality with zygotic gene expression.
Yuhkoh Satouh, Ken Sato
doaj   +1 more source

Haemogenic endocardium contributes to transient definitive haematopoiesis. [PDF]

, 2013
Haematopoietic cells arise from spatiotemporally restricted domains in the developing embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models suggest a close relationship among cardiac, endocardial and haematopoietic ...
Arshi, Armin, Conway, Simon J., Harmon, Andrew W., Harvey, Richard P., Liu, Xiaoqian, Mikkola, Hanna K. A., Nakano, Atsushi, Nakano, Haruko, Nakano, Haruko, Nakashima, Yasuhiro, Pashmforoush, Mohammad, Sasidharan, Rajkumar, Schwartz, Robert J., Shin, Jae-Ho, van Handel, Ben   +14 more
core   +1 more source

Epigenetic regulation in mammalian preimplantation embryo development

Reproductive Biology and Endocrinology, 2009
Preimplantation embryo development involves four stages: fertilization, cell cleavage, morula and blastocyst formation. During these stages, maternal and zygotic epigenetic factors play crucial roles.
Shi Lingjun, Wu Ji
doaj   +1 more source

A Tale of Division and Polarization in the Mammalian Embryo [PDF]

Developmental Cell, 2017
The first cell fate choice in mouse development is the segregation of the embryonic inner cell mass and the extra-embryonic trophectoderm. In this issue of Developmental Cell, Korotkevic and colleagues (2017) show that the interplay between cell polarization and cell-cell contact drives the segregation of these lineages, providing a framework for self ...
Srinivas, S, Rodriguez, TA
openaire   +5 more sources

Development of visible light‐sensitive human neuropsin (OPN5) via single amino acid substitution

FEBS Letters, EarlyView.
The present study determines a key amino acid residue, Lys91, for defining UV sensitivity of human OPN5. Heterologous action spectroscopy of the wild type and K91 mutants of OPN5 in HEK293T cells reveals that substitution of Lys91 with neutral (alanine) or acidic amino acids (glutamic or aspartic acids) causes substantial shifts in spectral sensitivity
Yusuke Sakai, Richard J. McDowell, Robert J. Lucas   +2 more
wiley   +1 more source