Results 51 to 60 of about 106,966 (240)

Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage

Cell Reports, 2017
Structure-specific endonucleases contribute to the maintenance of genome integrity by cleaving DNA intermediates that need to be resolved for faithful DNA repair, replication, or recombination.
Irene Saugar, Alberto Jiménez-Martín, José Antonio Tercero   +2 more
doaj   +1 more source

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes [PDF]

, 2003
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for ...
Belfort, M, Bestor, T, Bhagwat, A S, Bickle, T A, Bitinaite, J, Blumenthal, R M, Degtyarev, S K, Dryden, D T F, Dybvig, K, Firman, K, Gromova, E S, Gumport, R I, Halford, S E, Hattman, S, Heitman, J, Hornby, D P, Janulaitis, A, Jeltsch, A, Josephsen, J, Kiss, A, Klaenhammer, T R, Kobayashi, I, Kong, H M, Kruger, D H, Lacks, S, Marinus, M G, Miyahara, M, Morgan, R D, Murray, N E, Nagaraja, V, Piekarowicz, A, Pingoud, A, Raleigh, E, Rao, D N, Reich, N, Repin, V E, Roberts, R J, Selker, E U, Shaw, P C, Stein, D C, Stoddard, B L, Szybalski, W, Trautner, T A, Van Etten, J L, Vitor, J M B, Wilson, G G, Xu, S Y   +46 more
core   +2 more sources

Endonuclease II of coliphage T4: a recombinase disguised as a restriction endonuclease? [PDF]

Molecular Microbiology, 1998
EndoII shares with restriction endonucleases the property of cleaving foreign DNA while leaving the endonuclease‐encoding genome intact, ensuring the survival of one DNA species in the cell. In addition, in vivo EndoII cleaves a specific DNA sequence and cleavage is context dependent. These context effects extend over at least 1000 bp, largely limiting
Linda D. Kosturko, Karin Carlson
openaire   +2 more sources

Apurinic/apyrimidinic endonucleases of Mycobacterium tuberculosis protect against DNA damage but are dispensable for the growth of the pathogen in guinea pigs. [PDF]

PLoS ONE, 2014
In host cells, Mycobacterium tuberculosis encounters an array of reactive molecules capable of damaging its genome. Non-bulky DNA lesions are the most common damages produced on the exposure of the pathogen to reactive species and base excision repair ...
Rupangi Verma Puri, P Vineel Reddy, Anil K Tyagi   +2 more
doaj   +1 more source

Nucleosomes in serum as a marker for cell death [PDF]

, 2001
The concentration of nucleosomes is elevated in blood of patients with diseases which are associated with enhanced cell death. In order to detect these circulating nucleosomes, we used the Cell Death Detection-ELISA(Plus) (CDDE) from Roche Diagnostics ...
Anker P, Arends MJ, Burlingame RW, Du Clos TW, Emlen W, Gauthier VJ, Holdenrieder S, Jahr S, Leist M, Leon SA, Maebo A, Majno G, Mannherz HG, Rumore P, Stroun M, Watson RW   +15 more
core   +1 more source

Dissecting endonuclease and exonuclease activities in endonuclease V from Thermotoga maritima [PDF]

Nucleic Acids Research, 2010
Endonuclease V is an enzyme that initiates a conserved DNA repair pathway by making an endonucleolytic incision at the 3'-side 1 nt from a deaminated base lesion. DNA cleavage analysis using mutants defective in DNA binding and Mn(2+) as a metal cofactor reveals a novel 3'-exonuclease activity in endonuclease V [Feng,H., Dong,L., Klutz,A.M ...
Weiguo Cao, Rongjuan Mi, Anne K. Abole
openaire   +3 more sources

Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

eLife, 2022
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 provides a highly efficient and flexible genome editing technology with numerous potential applications ranging from gene therapy to population control.
Anna M Langmüller, Jackson Champer, Sandra Lapinska, Lin Xie, Matthew Metzloff, Samuel E Champer, Jingxian Liu, Yineng Xu, Jie Du, Andrew G Clark, Philipp W Messer   +10 more
doaj   +1 more source

Modular Nucleic Acid Assembled p/MHC Microarrays for Multiplexed Sorting of Antigen-Specific T Cells [PDF]

, 2009
The human immune system consists of a large number of T cells capable of recognizing and responding to antigens derived from various sources. The development of peptide-major histocompatibility (p/MHC) tetrameric complexes has enabled the direct ...
Bailey, Ryan C., Comin-Anduix, Begonya, Hadrup, Sine Reker, Heath, James R., Hwang, Kiwook, Koya, Richard C., Kwong, Gabriel A., Ma, Chao, Radu, Caius G., Ribas, Antoni, Schumacher, Ton N., Shu, Chengyi J., Witte, Owen N.   +12 more
core   +2 more sources

Novel Cloning Method for Recombinant Adenovirus Construction in Escherichia coli

BioTechniques, 1999
pAdvantage is a rapid cloning system for generating recombinant adenoviruses. The system is based on manipulating the fulllength adenovirus genome as a stable plasmid in E. coli using intron-encoded endonucleases.
David W. Souza, Donna Armentano
doaj   +1 more source

Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time-of-flight mass spectrometry [PDF]

, 2002
Nano-electrospray ionization time-of-flight mass spectrometry (ESI-MS) was used to study the conformational consequences of metal ion binding to the colicin E9 endonuclease (E9 DNase) by taking advantage of the unique capability of ESI-MS to allow ...
HECK, ALBERT J.R., James, Richard, JISKOOT, WIM, KLEANTHOUS, COLIN, MAIER, CLAUDIA S., Moore, Geoffrey R., VAN DEN BREMER, EWALD T.J.   +6 more
core   +3 more sources