Results 51 to 60 of about 155,310 (382)

A simple test for the cleavage activity of customized endonucleases in plants

Plant Methods, 2016
BackgroundAlthough customized endonucleases [transcription activator-like effector nucleases (TALENs) and RNA-guided endonucleases (RGENs)] are known to be effective agents of mutagenesis in various host plants, newly designed endonuclease constructs ...
N. Budhagatapalli, Sindy Schedel, M. Gurushidze, S. Pencs, S. Hiekel, T. Rutten, S. Kusch, Robert Morbitzer, T. Lahaye, R. Panstruga, J. Kumlehn, G. Hensel   +11 more
semanticscholar   +1 more source

Structure-Specific Endonucleases in DNA Repair

CHIMIA, 2009
Nucleotide excision repair (NER) is an essential DNA repair pathway that addresses a variety of DNA lesions formed by UV light, environmental mutagens and agents used in cancer chemotherapy.
Orlando D. Schärer
doaj   +1 more source

A novel zinc-finger nuclease platform with a sequence-specific cleavage module [PDF]

, 2011
Zinc-finger nucleases (ZFNs) typically consist of three to four zinc fingers (ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokI.
Cathomen, Toni, Dannemann, Nadine, Gabsalilow, Lilia, Pingoud, Alfred, Schierling, Benno, Wende, Wolfgang   +5 more
core   +2 more sources

F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif

Mobile DNA, 2018
Background There are six known families of homing endonucleases, LAGLIDADG, GIY-YIG, HNH, His-Cys box, PD-(D/E)-XK, and EDxHD, which are characterized by their conserved residues.
Xiaoting Fang, YongLiang Jiang, Kim Li, Qinglu Zeng   +3 more
doaj   +1 more source

Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases

Frontiers in Microbiology, 2020
Modification dependent restriction endonucleases (MDREs) restrict modified DNA, typically with limited sequence specificity (∼2–4 bp). Here, we focus on MDREs that have an SRA and/or SBD (sulfur binding domain) fused to an HNH endonuclease domain ...
Thomas Lutz, Honorata Czapinska, Alexey Fomenkov, Vladimir Potapov, Daniel F. Heiter, Bo Cao, Bo Cao, Peter Dedon, Matthias Bochtler, Matthias Bochtler, Shuang-yong Xu   +10 more
doaj   +1 more source

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

Genome Research, 2014
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9
Y. Sung, Jong Min Kim, Hyun-Taek Kim, Jaehoon Lee, Jisun Jeon, Young Jin, Jung-Hwa Choi, Y. Ban, S. Ha, Cheol-Hee Kim, Han-Woong Lee, Jin-Soo Kim   +11 more
semanticscholar   +1 more source

Genome editing technologies to fight infectious diseases [PDF]

, 2017
Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa, Pal\ue3\ub9, Giorgio, Trevisan, Marta   +2 more
core   +1 more source

SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres

Cell Reports, 2013
SLX4 interacts with several endonucleases to resolve structural barriers in DNA metabolism. SLX4 also interacts with telomeric protein TRF2 in human cells. The molecular mechanism of these interactions at telomeres remains unknown.
Bingbing Wan, Jinhu Yin, Kent Horvath, Jaya Sarkar, Yong Chen, Jian Wu, Ke Wan, Jian Lu, Peili Gu, Eun Young Yu, Neal F. Lue, Sandy Chang, Yie Liu, Ming Lei   +13 more
doaj   +1 more source

DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis. [PDF]

PLoS ONE, 2013
Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA ...
Ryushin Mizuta, Shinsuke Araki, Makoto Furukawa, Yuki Furukawa, Syota Ebara, Daisuke Shiokawa, Katsuhiko Hayashi, Sei-ichi Tanuma, Daisuke Kitamura   +8 more
doaj   +1 more source

Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions [PDF]

, 2013
During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation.
A Chavez, A De Muyt, A Hochwagen, A Hochwagen, A Irmisch, A McAleenan, A Schwacha, A Sourirajan, A Svetlanov, AC Chan, AJ Wood, AL Marston, Alex D. Herbert, Alice Copsey, Andreas Hochwagen, Andrew Chi-ho Chan, B Rockmill, BH Lee, C Buhler, C Tapia-Alveal, DM Sheedy, E Ampatzidou, EA Andrews, EA Outwin, ER Hoffmann, Eva Hoffmann, F Osman, FZ Watts, G De Piccoli, G Vader, GA Cromie, GR Smith, GV Borner, H Murakami, Hannah G. Blitzblau, HB Lindroos, HG Blitzblau, HG Blitzblau, HG Yu, HG Yu, I Lilienthal, J Gregan, J Matos, J Matos, J Palecek, J Pan, J Torres-Rosell, J Zalevsky, JA Carballo, JA Downs, JK Holloway, JM Murray, JR Mullen, JR Mullen, JS Bickel, K Nishimura, K Zakharyevich, K Zakharyevich, KA Henderson, KP Kim, KP Kohl, KT Nishant, L Jessop, L Jessop, L Newnham, L Wu, L Xu, LE Berchowitz, LE Hang, LJ Gaskell, Louise Newnham, M Bermudez-Lopez, M Xaver, MA Roy, Michael Lichten, MJ Neale, MN Boddy, MS McMahill, N Hunter, N Hunter, N Wu, Neil Hunter, NK Kolas, O Yildiz, P Jordan, Philip W. Jordan, PR Potts, PR Potts, Prakash Arumugam, RK Clyne, S Agarwal, S Chu, S Farmer, S Gray, S Panizza, S Pebernard, S Wehrkamp-Richter, SB Buonomo, SD Oh, SD Oh, Shangming Tang, SL Andersen, Sonya Newcombe, Stephen Gray, T de los Santos, T Snowden, T Wechsler, TM Carlile, TS Kitajima, V Kaliraman, WM Fricke, X Zhao, Y Blat, YH Chen, YH Chen, Zhaobo Li   +115 more
core   +3 more sources