Results 61 to 70 of about 60,825 (304)
A Cas9 with PAM recognition for adenine dinucleotides
Nature Communications, 2020 Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting ...
Pranam Chatterjee +7 more
doaj +1 more source
Type II restriction endonucleases: Structure and mechanism
, 2005 Type II restriction endonucleases are components of restriction modification systems that protect bacteria and archaea against invading foreign DNA.
Pingoud A. +3 more
core +1 more source
Advanced Science, EarlyView.This study elucidates the mechanisms of subcellular multidimensional collapse in exhausted T cells. By specifically targeting the nucleus, mitochondria, and endoplasmic reticulum, strategic interventions can effectively remodel the compromised organelle network. This integrated approach drives comprehensive T cell resuscitation, ultimately establishing
Mingxing Wang +9 more
wiley +1 more source
BioTechniques, 1999 REF Select, expert system software, has been developed to assist in the selection of optimal restriction endonucleases for restriction endonuclease fingerprinting (REF), a method for rapid and sensitive mutation screening of long DNA segments (1–2 kb ...
William A. Scaringe +3 more
doaj +1 more source
Tapping natural reservoirs of homing endonucleases for targeted gene modification
, 2011 Homing endonucleases mobilize their own genes by generating double-strand breaks at individual target sites within potential host DNA. Because of their high specificity, these proteins are used for “genome editing” in higher eukaryotes.
Barry L. Stoddard +8 more
core +1 more source
Advanced Science, EarlyView.ZDHHC17 palmitoylates CDK4, which together with TRAF6‐mediated ubiquitination, drives cell cycle progression and immune surveillance, revealing a rational combination of CDK4 inhibitors with immune checkpoint blockers for ZDHHC17‐driven cancers. ABSTRACT Uncontrolled cell cycle progression is a hallmark of cancer, tightly regulated by both intrinsic ...
Zekang Wang +7 more
wiley +1 more source
Nucleic Acid Therapeutics for “Undruggable” Cancer Targets: Mechanisms, Challenges, and Prospects
Advanced Science, EarlyView.Nucleic acid therapeutics bypass the structural limitations of conventional drugs by targeting mRNA rather than proteins. This review examines how antisense oligonucleotides, siRNAs, miRNAs, aptamers, and mRNA vaccines intervene against historically undruggable oncoproteins including Ras, MYC, and p53, highlighting mechanistic advances, delivery ...
Feng Xu +6 more
wiley +1 more source
EPIGENETICS TODAY AND TOMORROW
Вавиловский журнал генетики и селекции, 2015 Epigenetics is a science of inheritable organism properties that are not associated with changes in the DNA nucleotide sequence but can be indirectly encoded in the genome. The most known epigenetic mechanisms (signals) are enzymatic DNA methylation, the
B. F. Vanyushin
doaj
Targeted Mutagenesis Using RNA-guided Endonucleases in Mosses
Bio-Protocol, 2017 RNA-guided endonucleases (RGENs) have been used for genome editing in various organisms. Here, we demonstrate a simple method for performing targeted mutagenesis and genotyping in a model moss species, Physcomitrella patens, using RGENs.
Toshihisa Nomura, Hitoshi Sakakibara
doaj +1 more source
Topological digestion drives time-varying rheology of entangled DNA fluids
Nature Communications, 2022 Understanding and controlling the rheology of polymeric complex fluids is of fundamental importance in both industry and biology. Here, Michieletto et al. show how to achieve time-dependent rheology of DNA solutions via enzymatically-driven architectural
D. Michieletto +6 more
doaj +1 more source