Results 1 to 10 of about 6,098 (159)
Mutational and Kinetic Analysis of APE1 Endoribonuclease Activity [PDF]
Molecular Biology, 2021 Human apurinic/apyrimidinic endonuclease 1 (APE1) participates in the DNA repair system. It is believed that the main biological function of APE1 is Mg^(2+)-dependent hydrolysis of AP-sites in DNA. On the base of structural data, kinetic studies, and mutation analysis, the key stages of APE1 interaction with damaged DNA were established.
Alexandra A. Kuznetsova +5 more
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Colicin E3: A Unique Endoribonuclease [PDF]
Proceedings of the National Academy of Sciences, 1973 The question of whether or not a cellular ribonuclease is involved in the cleavage of 16S ribosomal RNA by colicin E3 was investigated. For this purpose ribosomes from strains devoid of some ribonucleases or ribosomes in which ribonucleases had been inactivated by heat or removed by extensive washings were used for the colicin reaction.
Irene Meyhack +2 more
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The Continuing Story of Endoribonuclease III [PDF]
Microbial Physiology, 2004 Endoribonuclease III (RNase III) plays an important role in the processing of rRNA and mRNAs. It is timely to summarize the most relevant insights obtained during the last years stemming from RNase III. With this aim, the present mini-review provides a wealth of new information focusing on the distribution and architecture of RNase III, substrate ...
Ciarán Condon, Djamel Drider
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Substrate specificity of human MCPIP1 endoribonuclease [PDF]
Scientific Reports, 2018 AbstractMCPIP1, also known as Regnase-1, is a ribonuclease crucial for regulation of stability of transcripts related to inflammatory processes. Here, we report that MCPIP1 acts as an endonuclease by degrading several stem-loop RNA structures and single-stranded RNAs.
Mateusz Wilamowski +3 more
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Structural Biology of the Arterivirus nsp11 Endoribonucleases [PDF]
Journal of Virology, 2017 ABSTRACT Endoribonuclease (NendoU) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. Arterivirus nonstructural protein 11 (nsp11) was shown to have NendoU activity and play essential roles in the viral life cycle.
Manfeng Zhang +7 more
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Substrate specificity of bacterial endoribonuclease toxins
BMB Reports, 2020 Bacterial endoribonuclease toxins belong to a protein family that inhibits bacterial growth by degrading mRNA or rRNA sequences. The toxin genes are organized in pairs with its cognate antitoxins in the chromosome and thus the activities of the toxins are antagonized by antitoxin proteins or RNAs during active translation.
Eunjin Lee, Yoontak Han
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Sequence-specific endoribonucleases
Postępy Biochemii, 2016 Ribonucleases are nucleolytic enzymes that commonly occur in living organisms and act by cleaving RNA molecules. These enzymes are involved in basic cellular processes, including the RNA maturation that accompanies the formation of functional RNAs, as well as RNA degradation that enables removal of defective or dangerous molecules or ones that have ...
Dawid, Głów +3 more
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Novel specificity of an endoribonuclease of yeast [PDF]
FEBS Letters, 1986 Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y‐A bonds of single‐stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonucleotides.
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An assay of ribonuclease H, endoribonucleases, and phosphatases. [PDF]
Proceedings of the National Academy of Sciences, 1976 An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely.
J G Starvianopoulos, E Chargaff
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Endoribonucleases associated with RNAs in chick embryos
FEBS Letters, 1979 proteins in hemopoietic rat cells were reported to have RNase activities [ 121. Similarly, an endogeneous endoribonucleolytic activity was found in the 50 S ribosomal subunit of Eschenihia coli [ 131. This paper describes the existence of a RNA-bound cytoplasmic endoribonuclease in chick embryo.
José La Torre +2 more
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