Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Anaerobic, mesophilic, and cellulolytic Ruminiclostridium cellulolyticum produces an efficient cellulolytic extracellular complex named cellulosome, which consist of a non-catalytic multi-functional integrating subunit, organizing the various ...
Na Wang +4 more
doaj +2 more sources
Targeted Control of Gene Expression Using CRISPR-Associated Endoribonucleases [PDF]
CellsCRISPR-associated endoribonucleases (Cas RNases) cleave single-stranded RNA in a highly sequence-specific manner by recognizing and binding to short RNA sequences known as direct repeats (DRs).
Sagar J. Parikh +8 more
doaj +2 more sources
Molecular basis for the calcium-dependent activation of the ribonuclease EndoU [PDF]
Nature CommunicationsRibonucleases (RNases) are ubiquitous enzymes that process or degrade RNA, essential for cellular functions and immune responses. The EndoU-like superfamily includes endoribonucleases conserved across bacteria, eukaryotes, and certain viruses, with an ...
Florian Malard +8 more
doaj +2 more sources
5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]
CellsDespite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh +6 more
doaj +2 more sources
Visual Evidence for the Recruitment of Four Enzymes with RNase Activity to the Bacillus subtilis Replication Forks [PDF]
CellsRemoval of RNA/DNA hybrids for the maturation of Okazaki fragments on the lagging strand, or due to misincorporation of ribonucleotides by DNA polymerases, is essential for all types of cells. In prokaryotic cells such as Escherichia coli, DNA polymerase
Rebecca Hinrichs, Peter L. Graumann
doaj +2 more sources
Global regulatory functions of the Staphylococcus aureus endoribonuclease III in gene expression. [PDF]
PLoS Genetics, 2012 RNA turnover plays an important role in both virulence and adaptation to stress in the Gram-positive human pathogen Staphylococcus aureus. However, the molecular players and mechanisms involved in these processes are poorly understood.
Efthimia Lioliou +7 more
doaj +3 more sources
Investigation of endoplasmic reticulum stress parameters in patients with gestational diabetes mellitus: A prospective study. [PDF]
Int J Gynaecol ObstetAbstract Objective Our aim in this study was to evaluate the levels of Activating Transcription Factor‐6 (ATF‐6), Inositol‐Requiring Enzyme 1 (IRE‐1), and C/EBP‐Homologous Protein (CHOP), which are critical markers of endoplasmic reticulum (ER) stress, in patients with gestational diabetes mellitus (GDM), a condition increasingly recognized for its ...
Sarıkaya S +6 more
europepmc +2 more sources
Using nucleolytic toxins as restriction enzymes enables new RNA applications. [PDF]
Nucleic Acids ResOver the past five decades, DNA restriction enzymes have revolutionized biotechnology. While these enzymes are widely used in DNA research and DNA engineering, the emerging field of RNA and mRNA therapeutics requires sequence-specific RNA ...
Rothweiler U +11 more
europepmc +3 more sources
A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags
Bio-Protocol, 2023 Interactions between RNA-binding proteins and RNA molecules are at the center of multiple biological processes. Therefore, accurate characterization of the composition of ribonucleoprotein complexes (RNPs) is crucial.
Violette Charteau +2 more
doaj +1 more source
mBio, 2021 Toxin-antitoxin (TA) systems are widespread genetic modules found in almost all bacteria that can regulate their growth and may play prominent roles in phage defense.
Peter H. Culviner +3 more
doaj +1 more source