Targeted Control of Gene Expression Using CRISPR-Associated Endoribonucleases [PDF]
CellsCRISPR-associated endoribonucleases (Cas RNases) cleave single-stranded RNA in a highly sequence-specific manner by recognizing and binding to short RNA sequences known as direct repeats (DRs).
Sagar J. Parikh +8 more
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Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Anaerobic, mesophilic, and cellulolytic Ruminiclostridium cellulolyticum produces an efficient cellulolytic extracellular complex named cellulosome, which consist of a non-catalytic multi-functional integrating subunit, organizing the various ...
Na Wang +4 more
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De novo design of hypercompact transcript degraders by engineering substrate-specific toxins and Cas6-CBS system [PDF]
Nature CommunicationsArtificial assembly of small functional proteins provides effective strategies for development of compact RNA degradation systems, which overcome the challenges associated with delivery.
Pin-Ru Chen +7 more
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5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]
CellsDespite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh +6 more
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Molecular basis for the calcium-dependent activation of the ribonuclease EndoU [PDF]
Nature CommunicationsRibonucleases (RNases) are ubiquitous enzymes that process or degrade RNA, essential for cellular functions and immune responses. The EndoU-like superfamily includes endoribonucleases conserved across bacteria, eukaryotes, and certain viruses, with an ...
Florian Malard +8 more
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QM/MM Free Energy Calculations of IRE1 Reveal a Unique Protonation State of the Catalytic Lys599. [PDF]
J Comput ChemDifferent conformations at the IRE1 kinase site, imposed by different inhibitors, lead to changes in interactions between the key catalytic residues which in turn stabilize Lysine 599 in its neutral (deprotonated) form. ABSTRACT Inositol Requiring Enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease identified as therapeutic ...
Carlesso A +5 more
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Visual Evidence for the Recruitment of Four Enzymes with RNase Activity to the Bacillus subtilis Replication Forks [PDF]
CellsRemoval of RNA/DNA hybrids for the maturation of Okazaki fragments on the lagging strand, or due to misincorporation of ribonucleotides by DNA polymerases, is essential for all types of cells. In prokaryotic cells such as Escherichia coli, DNA polymerase
Rebecca Hinrichs, Peter L. Graumann
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A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags
Bio-Protocol, 2023 Interactions between RNA-binding proteins and RNA molecules are at the center of multiple biological processes. Therefore, accurate characterization of the composition of ribonucleoprotein complexes (RNPs) is crucial.
Violette Charteau +2 more
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mBio, 2021 Toxin-antitoxin (TA) systems are widespread genetic modules found in almost all bacteria that can regulate their growth and may play prominent roles in phage defense.
Peter H. Culviner +3 more
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Scientific Reports, 2021 Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of intestinal pathogens responsible for a range of illnesses, including kidney failure and neurological compromise.
Patricia B. Lodato
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