Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis. [PDF]
FEBS LettThe S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Gilet L +4 more
europepmc +2 more sources
Using nucleolytic toxins as restriction enzymes enables new RNA applications. [PDF]
Nucleic Acids ResOver the past five decades, DNA restriction enzymes have revolutionized biotechnology. While these enzymes are widely used in DNA research and DNA engineering, the emerging field of RNA and mRNA therapeutics requires sequence-specific RNA ...
Rothweiler U +11 more
europepmc +3 more sources
Control of expression of the RNases J1 and J2 in bacillus subtilis [PDF]
, 2014 In Bacillus subtilis, the dual activity 5' exo- and endoribonucleases J1 and J2 are important players in mRNA and stable RNA maturation and degradation. Recent work has improved our understanding of their structure and mechanism of action and identified ...
Hébert, A. +3 more
core +1 more source
Drosophila CG3303 is an essential endoribonuclease linked to TDP-43-mediated neurodegeneration [PDF]
, 2017 Endoribonucleases participate in almost every step of eukaryotic RNA metabolism, acting either as degradative or biosynthetic enzymes. We previously identified the founding member of the Eukaryotic EndoU ribonuclease family, whose components display ...
BOZZONI, Irene +11 more
core +1 more source
Structural Principles of CRISPR RNA Processing [PDF]
, 2015 The Cas6 superfamily, the Cas5d subclass, and the host RNase III endoribonucleases are responsible for producing small RNAs (crRNA) that function in the CRISPR-Cas immunity.
Li, Hong
core +1 more source
Composition and conservation of the mRNA-degrading machinery in bacteria [PDF]
, 2011 RNA synthesis and decay counteract each other and therefore inversely regulate gene expression in pro- and eukaryotic cells by controlling the steady-state level of individual transcripts.
Dharam Singh +2 more
core +4 more sources
Functional implications of hexameric dynamics in SARS-CoV-2 Nsp15. [PDF]
Protein SciAbstract SARS‐CoV‐2, the virus responsible for the COVID‐19 pandemic, has undergone continuous evolution, leading to the emergence of variants with altered transmissibility and immune evasion. For the non‐structural proteins (Nsps) of SARS‐CoV‐2, there are limited structural analyses of their naturally occurring mutations.
Sonowal M +18 more
europepmc +2 more sources
Ribonucleases J1 and J2: two novel endoribonucleases in B.subtilis with functional homology to E.coli RNase E [PDF]
, 2005 Many prokaryotic organisms lack an equivalent of RNase E, which plays a key role in mRNA degradation in Escherichia coli. In this paper, we report the purification and identification by mass spectrometry in Bacillus subtilis of two paralogous ...
Bréchemmier-Baey, Dominique +6 more
core +2 more sources
Pdxdc1 modulates prepulse inhibition of acoustic startle in the mouse. [PDF]
, 2017 Current antipsychotic medications used to treat schizophrenia all target the dopamine D2 receptor. Although these drugs have serious side effects and limited efficacy, no novel molecular targets for schizophrenia treatment have been successfully ...
Boutros, PC +5 more
core +1 more source
Regulation of the human tRNase ZS gene expression [PDF]
, 2008 There are two types of tRNA 3′ processing endoribonucleases (tRNase Z): a short form (tRNase ZS) and a long form (tRNase ZL). Although the human genome contains both genes, little is known about the physiological role of tRNase ZS.
Nashimoto, Masayuki +2 more
core +1 more source