Results 21 to 30 of about 9,566 (262)

Regulation of 6S RNA biogenesis by switching utilization of both sigma factors and endoribonucleases [PDF]

Nucleic Acids Research, 2004
In Escherichia coli, 6S RNA functions as a modulator of RNA polymerase sigma70-holoenzyme activity, but its biosynthetic pathway remains uncharacterized.
Kwang-sun Kim
openalex   +2 more sources

Selective Characterization of mRNA 5′ End-Capping by DNA Probe-Directed Enrichment with Site-Specific Endoribonucleases

ACS Pharmacology & Translational Science, 2023
The N7-methyl guanosine cap structure is an essential 5′ end modification of eukaryotic mRNA. It plays a critical role in many aspects of the life cycle of mRNA, including nuclear export, stability, and translation. Equipping synthetic transcripts with a
Eric J. Wolf, Nan Dai, S. Chan, Ivan R. Corrêa   +3 more
semanticscholar   +1 more source

A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis. [PDF]

Nucleic Acids Res
RNA endonucleases are the rate-limiting initiator of decay for many bacterial mRNAs. However, the positions of cleavage and their sequence determinants remain elusive even for the well-studied Bacillus subtilis.
Taggart JC, Dierksheide KJ, LeBlanc HJ, Lalanne JB, Durand S, Braun F, Condon C, Li GW.   +7 more
europepmc   +2 more sources

PIN and CCCH Zn-finger domains coordinate RNA targeting in ZC3H12 family endoribonucleases

Nucleic Acids Research, 2021
The CCCH-type zinc finger (ZnF) containing ZC3H12 ribonucleases are crucial in post-transcriptional immune homoeostasis with ZC3H12A being the only structurally studied member of the family.
A. Garg, Y. Roske, Shinnosuke Yamada, Takuya Uehata, O. Takeuchi, U. Heinemann   +5 more
semanticscholar   +1 more source

A Novel Endoribonuclease, RNase LS, in Escherichia coli [PDF]

Genetics, 2005
Abstract The dmd gene of bacteriophage T4 is required for the stability of late-gene mRNAs. When this gene is mutated, late genes are globally silenced because of rapid degradation of their mRNAs. Our previous work suggested that a novel Escherichia coli endonuclease, RNase LS, is responsible for the rapid degradation of mRNAs.
Yuichi Otsuka, Tetsuro Yonesaki
openalex   +5 more sources

Sequence-specific endoribonucleases

Postępy Biochemii, 2016
Ribonucleases are nucleolytic enzymes that commonly occur in living organisms and act by cleaving RNA molecules. These enzymes are involved in basic cellular processes, including the RNA maturation that accompanies the formation of functional RNAs, as well as RNA degradation that enables removal of defective or dangerous molecules or ones that have ...
Dawid Głów, Martyna Nowacka, Krzysztof Skowronek, Janusz M. Bujnicki   +3 more
openalex   +4 more sources

Mutational and Kinetic Analysis of APE1 Endoribonuclease Activity [PDF]

Molecular Biology, 2021
Human apurinic/apyrimidinic endonuclease 1 (APE1) participates in the DNA repair system. It is believed that the main biological function of APE1 is Mg^(2+)-dependent hydrolysis of AP-sites in DNA. On the base of structural data, kinetic studies, and mutation analysis, the key stages of APE1 interaction with damaged DNA were established.
Alexandra A. Kuznetsova, Olga S. Fedorova, D. S. Novopashina, A. A. Gavrilova, A. A. Gavrilova, Nikita A. Kuznetsov   +5 more
openaire   +3 more sources

Human RNase 4 improves mRNA sequence characterization by LC-MS/MS. [PDF]

Nucleic Acids Res, 2022
With the rapid growth of synthetic messenger RNA (mRNA)-based therapeutics and vaccines, the development of analytical tools for characterization of long, complex RNAs has become essential.
Wolf EJ, Grünberg S, Dai N, Chen TH, Roy B, Yigit E, Corrêa IR.   +6 more
europepmc   +2 more sources

Colicin E3: A Unique Endoribonuclease [PDF]

Proceedings of the National Academy of Sciences, 1973
The question of whether or not a cellular ribonuclease is involved in the cleavage of 16S ribosomal RNA by colicin E3 was investigated. For this purpose ribosomes from strains devoid of some ribonucleases or ribosomes in which ribonucleases had been inactivated by heat or removed by extensive washings were used for the colicin reaction.
Irene Meyhack, Bernd Meyhack, David Apirion   +2 more
openaire   +3 more sources

The Continuing Story of Endoribonuclease III [PDF]

Microbial Physiology, 2004
Endoribonuclease III (RNase III) plays an important role in the processing of rRNA and mRNAs. It is timely to summarize the most relevant insights obtained during the last years stemming from RNase III. With this aim, the present mini-review provides a wealth of new information focusing on the distribution and architecture of RNase III, substrate ...
Ciarán Condon, Djamel Drider
openaire   +2 more sources