Results 21 to 30 of about 5,190 (143)
Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
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Substrate specificity of bacterial endoribonuclease toxins
BMB Reports, 2020 Bacterial endoribonuclease toxins belong to a protein family that inhibits bacterial growth by degrading mRNA or rRNA sequences. The toxin genes are organized in pairs with its cognate antitoxins in the chromosome and thus the activities of the toxins are antagonized by antitoxin proteins or RNAs during active translation.
Han, Yoontak, Lee, Eun-Jin
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Structural Biology of the Arterivirus nsp11 Endoribonucleases [PDF]
Journal of Virology, 2017 ABSTRACT Endoribonuclease (NendoU) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. Arterivirus nonstructural protein 11 (nsp11) was shown to have NendoU activity and play essential roles in the viral life cycle.
Manfeng Zhang +7 more
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Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
Nature Communications, 2019 MazF endoribonucleases are thought to arrest growth of Mycobacterium tuberculosis by global translation inhibition. Here, Barth et al. show that MazF-mt9 cleaves a specific tRNA, leading to ribosome stalling at AAA codons and thus selective mRNA ...
Valdir C. Barth +5 more
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Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
mLife, 2023 Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression. A key endoribonuclease in Gram‐negative bacteria is RNase E.
Satoru Watanabe +10 more
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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H [PDF]
The Korean Journal of Parasitology, 2017 Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters.
Lim, Sung-Bin +9 more
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Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions
Microbiology Spectrum, 2021 Dicer-like proteins (DCLs) play a vital role in RNA interference (RNAi), by cleaving RNA filament into small RNAs. Although DCL-mediated RNAi can regulate interspecific communication between pathogenic/mutualistic organisms and their hosts, its role in ...
Edoardo Piombo +7 more
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Global regulatory functions of the Staphylococcus aureus endoribonuclease III in gene expression. [PDF]
PLoS Genetics, 2012 RNA turnover plays an important role in both virulence and adaptation to stress in the Gram-positive human pathogen Staphylococcus aureus. However, the molecular players and mechanisms involved in these processes are poorly understood.
Efthimia Lioliou +7 more
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The Continuing Story of Endoribonuclease III
Microbial Physiology, 2004 Endoribonuclease III (RNase III) plays an important role in the processing of rRNA and mRNAs. It is timely to summarize the most relevant insights obtained during the last years stemming from RNase III. With this aim, the present mini-review provides a wealth of new information focusing on the distribution and architecture of RNase III, substrate ...
Djamel, Drider, Ciarán, Condon
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Global analyses of small interfering RNAs derived from Bamboo mosaic virus and its associated satellite RNAs in different plants. [PDF]
PLoS ONE, 2010 BACKGROUND: Satellite RNAs (satRNAs), virus parasites, are exclusively associated with plant virus infection and have attracted much interest over the last 3 decades.
Kuan-Yu Lin +8 more
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