Results 21 to 30 of about 5,849 (189)
Mutational and Kinetic Analysis of APE1 Endoribonuclease Activity [PDF]
Molecular Biology, 2021 Human apurinic/apyrimidinic endonuclease 1 (APE1) participates in the DNA repair system. It is believed that the main biological function of APE1 is Mg^(2+)-dependent hydrolysis of AP-sites in DNA. On the base of structural data, kinetic studies, and mutation analysis, the key stages of APE1 interaction with damaged DNA were established.
Kuznetsova, A. A. +4 more
openaire +3 more sources
Sinorhizobium meliloti RNase III: Catalytic Features and Impact on Symbiosis
Frontiers in Genetics, 2018 Members of the ribonuclease (RNase) III family of enzymes are metal-dependent double-strand specific endoribonucleases. They are ubiquitously found and eukaryotic RNase III-like enzymes include Dicer and Drosha, involved in RNA processing and RNA ...
Margarida Saramago +4 more
doaj +1 more source
Sequence-specific endoribonucleases
Postępy Biochemii, 2016 Ribonucleases are nucleolytic enzymes that commonly occur in living organisms and act by cleaving RNA molecules. These enzymes are involved in basic cellular processes, including the RNA maturation that accompanies the formation of functional RNAs, as well as RNA degradation that enables removal of defective or dangerous molecules or ones that have ...
Dawid, Głów +3 more
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Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
doaj +1 more source
Substrate specificity of bacterial endoribonuclease toxins
BMB Reports, 2020 Bacterial endoribonuclease toxins belong to a protein family that inhibits bacterial growth by degrading mRNA or rRNA sequences. The toxin genes are organized in pairs with its cognate antitoxins in the chromosome and thus the activities of the toxins are antagonized by antitoxin proteins or RNAs during active translation.
Han, Yoontak, Lee, Eun-Jin
openaire +3 more sources
Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
mLife, 2023 Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression. A key endoribonuclease in Gram‐negative bacteria is RNase E.
Satoru Watanabe +10 more
doaj +1 more source
Structural Biology of the Arterivirus nsp11 Endoribonucleases [PDF]
Journal of Virology, 2017 ABSTRACT Endoribonuclease (NendoU) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. Arterivirus nonstructural protein 11 (nsp11) was shown to have NendoU activity and play essential roles in the viral life cycle.
Manfeng Zhang +7 more
openaire +2 more sources
Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
Nature Communications, 2019 MazF endoribonucleases are thought to arrest growth of Mycobacterium tuberculosis by global translation inhibition. Here, Barth et al. show that MazF-mt9 cleaves a specific tRNA, leading to ribosome stalling at AAA codons and thus selective mRNA ...
Valdir C. Barth +5 more
doaj +1 more source
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H [PDF]
The Korean Journal of Parasitology, 2017 Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters.
Lim, Sung-Bin +9 more
openaire +2 more sources
Angewandte Chemie, EarlyView.Here, we transform this otherwise destructive enzymatic activity into a powerful diagnostic advantage through an RNase I–assisted rolling circle amplification (RI‐RCA) strategy. By integrating controlled RNase I–mediated RNA digestion with circular DNA templates, this approach enables direct and highly sensitive detection of target RNA sequences. Using
Amal Mathai +5 more
wiley +2 more sources