Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis sp. PCC 6803
mLife, 2023 Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression. A key endoribonuclease in Gram‐negative bacteria is RNase E.
Satoru Watanabe +10 more
doaj +1 more source
Pdxdc1 modulates prepulse inhibition of acoustic startle in the mouse. [PDF]
, 2017 Current antipsychotic medications used to treat schizophrenia all target the dopamine D2 receptor. Although these drugs have serious side effects and limited efficacy, no novel molecular targets for schizophrenia treatment have been successfully ...
Boutros, PC +5 more
core +1 more source
Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal putative regulators with a potential role in carbon and iron metabolism [PDF]
, 2017 Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not ...
Coenye, Tom +2 more
core +3 more sources
Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
doaj +1 more source
Impact of alternative carbon sources and antifungal treatment on Candida glabrata biofilms transcription profile [PDF]
, 2016 Candida glabrata is considered a major opportunistic fungal pathogen of humans and has emerged as a leading cause of nosocomial fungal infections. The capacity of this yeast species to cause infections is dependent on the ability to grow within the human
Alves, Rosana +8 more
core +1 more source
Novel specificity of an endoribonuclease of yeast [PDF]
FEBS Letters, 1986 Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y‐A bonds of single‐stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonucleotides.
openaire +3 more sources
Composition and conservation of the mRNA-degrading machinery in bacteria [PDF]
, 2011 RNA synthesis and decay counteract each other and therefore inversely regulate gene expression in pro- and eukaryotic cells by controlling the steady-state level of individual transcripts.
Dharam Singh +2 more
core +4 more sources
Toxin-mediated ribosome stalling reprograms the Mycobacterium tuberculosis proteome
Nature Communications, 2019 MazF endoribonucleases are thought to arrest growth of Mycobacterium tuberculosis by global translation inhibition. Here, Barth et al. show that MazF-mt9 cleaves a specific tRNA, leading to ribosome stalling at AAA codons and thus selective mRNA ...
Valdir C. Barth +5 more
doaj +1 more source
A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]
, 2015 RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia +4 more
core +2 more sources
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
RNA: A publication of the RNA Society, 2010 For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
R. Tomecki, A. Dziembowski
semanticscholar +1 more source