Results 61 to 70 of about 6,098 (159)

Messenger RNA degradation is initiated at the 5′ end and follows sequence- and condition-dependent modes in chloroplasts [PDF]

Using reporter gene constructs, consisting of the bacterial uidA (GUS) coding region flanked by the 5′ and 3′ regions of the Chlamydomonas rbcL and psaB genes, respectively, we studied the degradation of mRNAs in the chloroplast of Chlamydomonas ...
Anthonisen, Blowers, Blowers, Bollenbach, Bouvet, Boynton, Deana, Decker, Deutscher, Drager, Even, Goldschmidt-Clermont, Higgs, Jacobs, Kime, Klein, L. Suay, M. L. Salvador, Mathy, Nakamura, Nickelsen, Pfalz, Saha, Salvador, Salvador, Salvador, Salvador, Stern, Suay, U. Klein, Zicker, Zimmer   +31 more
core   +2 more sources

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions [PDF]

, 2011
Prokaryotes have evolved multiple versions of an RNA-guided adaptive immune system that targets foreign nucleic acids. In each case, transcripts derived from clustered regularly interspaced short palindromic repeats (CRISPRs) are thought to selectively ...
Barendregt, Arjan, Boekema, Egbert, Bultema, Jelle, Dickman, Mark, Doudna, Jennifer A., Heck, Albert, van Duijn, Esther, Waghmare, Sakharam, Westphal, Wiebke, Wiedenheft, Blake, Zhou, Kaihong   +10 more
core   +4 more sources

Controlling mRNA stability and translation with the CRISPR endoribonuclease Csy4 [PDF]

RNA, 2015
The bacterial CRISPR endoribonuclease Csy4 has recently been described as a potential RNA processing tool. Csy4 recognizes substrate RNA through a specific 28-nt hairpin sequence and cleaves at the 3′ end of the stem. To further explore applicability in mammalian cells, we introduced this hairpin at various locations in mRNAs derived from reporter ...
William F. Marzluff, Patrick E. Lackey, Michael Huang, Aravind Asokan, Erin K. Borchardt, Leonidas A. Vandoros   +5 more
openaire   +3 more sources

Identification of Apurinic/apyrimidinic endonuclease 1 (APE1) as the endoribonuclease that cleaves c-myc mRNA [PDF]

Endonucleolytic cleavage of the coding region determinant (CRD) of c-myc mRNA appears to play a critical role in regulating c-myc mRNA turnover. Using 32P-labeled c-myc CRD RNA as substrate, we have purified and identified two endoribonucleases from rat ...
A. K. Mantha, Barzilay, Barzilay, Bassett, Bergstrom, Bernstein, Berquist, Binder, Bollenbach, Brewer, C. H. Lee, Chattopadhyay, Chou, Claverie-Martin, Cunningham, Dodson, Eberle, Evans, Fan, Gatfield, Heise, Herrick, Ioannidis, Izumi, Kuninger, Lebreton, Lee, Lemm, Levens, LINGEL, Mitra, Morello, Rodriguez, S. Mitra, S.-E. Kim, Schaeffer, Schmittgen, Shih, Swartwout, T. Barnes, T. Izumi, Tafech, Tell, Tirasophon, Tourriere, van Dijk, Vascotto, W.-C. Kim, Wang, Wisdom, Yeilding   +50 more
core   +2 more sources

Human 5’-tailed Mirtrons are Processed by RNaseP [PDF]

, 2021
Approximately a thousand microRNAs (miRNAs) are documented from human cells. A third appear to transit non-canonical pathways that typically bypass processing by Drosha, the dedicated nuclear miRNA producing enzyme.
Zia, Mohammad Farid
core   +1 more source

Advancing the Identification of Bioactive Molecules and the Construction of a Synergistic Drug Delivery System in Combating Lung Injury

Advanced Science, Volume 12, Issue 18, May 15, 2025.
Traditional Chinese Medicine (TCM), while holistic and historically esteemed, faces challenges in “miracle cures” due to slow onset, long cycles, and difficulty controlling quality. This study obtains the active ingredients, glabridin (GLA) and puerarin (PUE), from Ge‐Gen Decoction (GGD), developing a safe and effective drug delivery system, GLA‐PUE ...
Jianhong Qi, Yanxia Wang, Huan Chen, Kaitian Wu, Pei Zhou, Yue Dou, Bingqi Xiong, Wei Zhou   +7 more
wiley   +1 more source

Mechanism of substrate selection by a highly specific CRISPR endoribonuclease [PDF]

RNA, 2012
Bacteria and archaea possess adaptive immune systems that rely on small RNAs for defense against invasive genetic elements. CRISPR (clustered regularly interspaced short palindromic repeats) genomic loci are transcribed as long precursor RNAs, which must be enzymatically cleaved to generate mature CRISPR-derived RNAs (crRNAs) that serve as guides for ...
Sternberg, Samuel H, Haurwitz, Rachel E, Doudna, Jennifer A   +2 more
openaire   +5 more sources

The Relevance of Ribonuclease III in Pathogenic Bacteria [PDF]

, 2013
Dissertation presented to obtain the Ph.D degree in Biology.Ribonucleases (RNases) are key factors in the control of all biological processes, since they modulate the stability of RNA transcripts, allowing rapid changes in gene expression.
Saramago, Ana Margarida Teixeira
core  

The Long and Short of MicroRNA [PDF]

, 2013
MicroRNAs (miRNAs) are versatile regulators of gene expression in higher eukaryotes. In order to silence many different mRNAs in a precise manner, miRNA stability and efficacy is controlled by highly developed regulatory pathways and fine-tuning ...
Gilbert, Robert J.C., Norbury, Chris J., Yates, Luke A.   +2 more
core   +1 more source

Regulation and Function of the Atypical IκBs—Bcl‐3, IκBNS, and IκBζ—in Lymphocytes and Autoimmunity

European Journal of Immunology, Volume 55, Issue 5, May 2025.
This review article summarizes the currently available information on the functions of atypical IκB proteins including Bcl‐3, IκBNS, and IκBζ, which have been studied mostly in mouse models. We focus on the regulation of these modulators of transcription, describe their role in lymphocyte differentiation, and their involvement in the development of ...
Tanja Kübelbeck, Nina Olivera Wichmann, Timsse Raj, Cynthia Raj, Caspar Ohnmacht, Nadine Hövelmeyer, Daniela Kramer, Vigo Heissmeyer   +7 more
wiley   +1 more source