Acta Pharmaceutica Sinica B, 2021 The functionality of DNA biomacromolecules has been widely excavated, as therapeutic drugs, carriers, and functionalized modification derivatives.
Xiu Han +4 more
doaj +1 more source
"Alternative" endocytic mechanisms exploited by pathogens: new avenues for therapeutic delivery? [PDF]
, 2007 Some pathogens utilize unique routes to enter cells that may evade the intracellular barriers encountered by the typical clathrin-mediated endocytic pathway.
Medina-Kauwe, LK
core +1 more source
Endosomal escape for cell-targeted proteins. Going out after going in.
Biotechnology Advances, 2023 Protein-based nanocarriers are versatile and biocompatible drug delivery systems. They are of particular interest in nanomedicine as they can recruit multiple functions in a single modular polypeptide.
Eric Voltà-Durán +5 more
semanticscholar +1 more source
Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins. [PDF]
, 2018 New methods for delivering proteins into the cytosol of mammalian cells are being reported at a rapid pace. Differentiating between these methods in a quantitative manner is difficult, however, as most assays for evaluating cytosolic protein delivery are
Knox, Susan L +4 more
core +2 more sources
Role of liposome and peptide in the synergistic enhancement of transfection with a lipopolyplex vector [PDF]
, 2015 Lipopolyplexes are of widespread interest for gene therapy due to their multifunctionality and high transfection efficiencies. Here we compared the biological and biophysical properties of a lipopolyplex formulation with its lipoplex and polyplex ...
A Elouahabi +31 more
core +1 more source
Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts [PDF]
, 2020 Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target ...
Braeckmans, Kevin +6 more
core +1 more source
Lipid nanoparticle topology regulates endosomal escape and delivery of RNA to the cytoplasm
bioRxiv, 2022 RNA therapeutics have the potential to resolve a myriad of diseases caused by gene deficiency. Lipid nanoparticles (LNPs) are one of the most successful RNA delivery systems. However, expanding their application hinges on the discovery of next generation
Lining Zheng +2 more
semanticscholar +1 more source
Viral escape from endosomes and host detection at a glance [PDF]
Journal of Cell Science, 2018 ABSTRACT In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endosomal ...
Jacqueline Staring +2 more
openaire +2 more sources
Nuclear inclusion of nontargeted and chromatin-targeted polystyrene beads and plasmid DNA containing nanoparticles [PDF]
, 2011 The nuclear membrane is one of the major cellular barriers in the delivery of plasmid DNA (pDNA). Cell division has a positive influence on the expression efficiency since, at the end of mitosis, pDNA or pDNA containing complexes near the chromatin are ...
De Smedt, Stefaan +5 more
core +1 more source
Spatiotemporal Dynamics of Adenovirus Membrane Rupture and Endosomal Escape [PDF]
Journal of Virology, 2012 ABSTRACT A key step in adenovirus cell entry is viral penetration of cellular membranes to gain access to the cytoplasm and deliver the genome to the nucleus. Yet little is known about this important event in the adenoviral life cycle.
Oana, Maier +4 more
openaire +2 more sources