Results 241 to 250 of about 4,995,766 (296)

Human Alu loci gain enhancer-type epigenetic modifications in monocytes during response to bacterial LPS. [PDF]

Epigenetics Chromatin
Pan Y, Ichiyanagi K.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

ELF5-Mediated Enhancer-Promoter Interaction Regulates LALBA Expression in Ovine Mammary Gland. [PDF]

Adv Sci (Weinh)
Wu X, Wang J, Hao Z, Li M, Zhen H, Liu Y, Zhao G, Ren C, Zhou X, Hu H, Li M, Shi N.   +11 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Unlocking the Regulatory Genome: Interpreting the Clinical Impact of Noncoding Variants in Genetic Cardiomyopathies. [PDF]

Circ Genom Precis Med
Renberg A, Coppersmith S, Helms AS.
europepmc   +1 more source

Lignin-correlated suppression of mobile genetic elements (MGEs) and virulence factors (VFs) in swine bedding microenvironment and its association with immune enhancement

Environmental Technology & Innovation
Zhonghui Wang, Yameng Li, Zhiyang Zhang, Zhihao Zhang, Ying Qian, Yiping Guo, Jinxia Fan, Honggui Liu, Liquan Ren   +8 more
openaire   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Conservation of Long G4-rich (LG4) genomic enhancer regulations. [PDF]

BMC Genomics
Shaw MH, DeMeis JD, Arnold CA, Cox MR, Duong TC, Gaviria KA, McDavid GK, Villegas JM, Weimer ML, Patil SS, Alqudah SY, Borchert GM.   +11 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source