A long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mRNA in murine cytomegalovirus [PDF]
, 1985 Using the simian virus 40 "enhancer trap" approach, we have identified a transcription enhancer located just upstream of the major immediate early gene of murine cytomegalovirus. This enhancer has several striking properties.
Dorsch-Häsler, Karoline +5 more
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cis-regulatory circuits regulating NEK6 kinase overexpression in transformed B cells Are super-enhancer independent [PDF]
, 2017 Alterations in distal regulatory elements that control gene expression underlie many diseases, including cancer. Epigenomic analyses of normal and diseased cells have produced correlative predictions for connections between dysregulated enhancers and ...
Huang, Yue +6 more
core +3 more sources
Nascent RNA sequencing analysis provides insights into enhancer-mediated gene regulation
BMC Genomics, 2018 Background Enhancers are distal cis-regulatory elements that control gene expression. Despite an increasing appreciation of the importance of enhancers in cellular function and disease, our knowledge of enhancer-regulated transcription is very limited ...
Jing Wang +5 more
doaj +1 more source
Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells. [PDF]
, 2017 Endothelial cells (ECs) are critical determinants of vascular homeostasis and inflammation, but transcriptional mechanisms specifying their identities and functional states remain poorly understood.
Glass, Christopher K +7 more
core +2 more sources
Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function. [PDF]
, 2012 A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic ...
Hiraoka, Kei +3 more
core +2 more sources
Long non-coding RNAs and enhancers [PDF]
Current Opinion in Genetics & Development, 2011 Long non-coding RNAs (ncRNAs) are emerging as important regulatory factors in mammalian genomics. A number of reports within the last 2 years have identified thousands of actively expressed long ncRNA transcripts with distinct properties. The long ncRNAs show differential expression patterns and regulation in a wide variety of cells and tissues, adding
Ulf Andersson, Ørom, Ramin, Shiekhattar
openaire +2 more sources
Haematologica, 2021 Enhancers are regulatory regions of DNA, which play a key role in cell-type specific differentiation and development. Most active enhancers are transcribed into enhancer RNA (eRNA) that can regulate transcription of target genes by means of in cis as ...
Sara Napoli +17 more
doaj +1 more source
Interleukin 2 transcription factors as molecular targets of cAMP inhibition: delayed inhibition kinetics and combinatorial transcription roles [PDF]
, 1994 Elevation of cAMP can cause gene-specific inhibition of interleukin 2 (IL-2) expression. To investigate the mechanism of this effect, we have combined electrophoretic mobility shift assays and in vivo genomic footprinting to assess both the availability ...
Chen, Dan, Rothenberg, Ellen V.
core +2 more sources
Enhancer RNAs: the new molecules of transcription [PDF]
Current Opinion in Genetics & Development, 2014 In the past few years, technological advances in nucleotide sequencing have culminated in a greater understanding of the complexity of the human transcriptome. Notably, the discovery that distal regulatory elements known as enhancers are transcribed and such enhancer-derived transcripts (eRNAs) serve a critical function in transcriptional activation ...
Fan, Lai, Ramin, Shiekhattar
openaire +2 more sources
Transcriptional landscape and clinical utility of enhancer RNAs for eRNA-targeted therapy in cancer
Nature Communications, 2019 Enhancer RNA (eRNA) is a type of noncoding RNA transcribed from enhancer regions. Here, the authors investigate the transcriptional landscape of eRNA in cancer, incorporating pharmacogenomic data to identify potential target genes and therapeutic targets.
Zhao Zhang +14 more
doaj +1 more source