Results 81 to 90 of about 1,506,434 (336)

On a roll: a direct comparison of extraction methods for the recovery of eDNA from roller swabbing of surfaces

open access: yesBMC Research Notes, 2023
Objective Roller swabbing of surfaces is an effective way to obtain environmental DNA, but the current DNA extraction method for these samples is equipment heavy, time consuming, and increases potential contamination through multiple handling.
Austin M. Guthrie   +4 more
doaj   +1 more source

Identification of functional cis-regulatory elements by sequential enrichment from a randomized synthetic DNA library [PDF]

open access: yes, 2013
BACKGROUND: The identification of endogenous cis-regulatory DNA elements (CREs) responsive to endogenous and environmental cues is important for studying gene regulation and for biotechnological applications but is labor and time intensive. Alternatively,
Ahmadinejad, N.   +3 more
core   +2 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Insight into Invertebrate Community in Solonchak Soil Type Using EDNA Metabarcoding – A Pilot Study

open access: yesContemporary Agriculture
Solonchaks are soils characterized by high concentrations of soluble salts. These soils form unique ecosystems that provide habitats for organisms adapted to such extreme conditions.
Lazić Cvijeta   +9 more
doaj   +1 more source

The applicability of eDNA metabarcoding approaches for sessile benthic surveying in the Kimberley region, north‐western Australia

open access: yesEnvironmental DNA, 2022
The application of environmental DNA technologies is a promising new approach to rapidly audit biodiversity across large‐scale, remote regions. Here, we examine the efficacy of a dual‐assay eDNA metabarcoding approach for sessile benthic bioassessments ...
Katrina M. West   +6 more
doaj   +1 more source

A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations [PDF]

open access: yes, 2017
© 2017 Sellers et al. The tadpole shrimp, Triops cancriformis, is a freshwater crustacean listed as endangered in the UK and Europe living in ephemeral pools.
Griffin, Larry R.   +3 more
core   +3 more sources

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

open access: yesFEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom   +3 more
wiley   +1 more source

The European Catfish (Silurus glanis) as an Invasive Species – eDNA Detection Methods

open access: yesScientific Papers Animal Science and Biotechnologies, 2023
The European or Wels catfish (Silurus glanis) is an opportunistic apex predator which is the largest freshwater fish species in Europe, making it a popular choice as an angler fish or biological control agent.
Bettina Hegedűs   +3 more
doaj  

Syntheses and characterizations of the in vivo replicative bypass and mutagenic properties of the minor-groove O2-alkylthymidine lesions. [PDF]

open access: yes, 2014
Endogenous metabolism, environmental exposure, and treatment with some chemotherapeutic agents can all give rise to DNA alkylation, which can occur on the phosphate backbone as well as the ring nitrogen or exocyclic nitrogen and oxygen atoms of ...
Cai, Qian   +3 more
core   +1 more source

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