Results 71 to 80 of about 1,505,378 (311)

Persistence of environmental DNA in marine systems [PDF]

Communications Biology, 2018
AbstractAs environmental DNA (eDNA) becomes an increasingly valuable resource for marine ecosystem monitoring, understanding variation in its persistence across contrasting environments is critical. Here, we quantify the breakdown of macrobial eDNA over a spatio-temporal axis of locally extreme conditions, varying from ocean-influenced offshore to ...
Rupert A. Collins, Owen S. Wangensteen, Eoin J. O’Gorman, Stefano Mariani, David W. Sims, Martin J. Genner   +5 more
openaire   +7 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

Computational and Structural Biotechnology Journal, 2021
Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments ...
Mohamed A. Sobhy, Muhammad Tehseen, Masateru Takahashi, Amer Bralić, Alfredo De Biasio, Samir M. Hamdan   +5 more
doaj   +1 more source

Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity. [PDF]

, 2018
Here we introduce Protein-indexed Assay of Transposase Accessible Chromatin with sequencing (Pi-ATAC) that combines single-cell chromatin and proteomic profiling. In conjunction with DNA transposition, the levels of multiple cell surface or intracellular
Chang, Howard Y, Chen, Xingqi, Choudhry, Hani, Giaccia, Amato J, Greenleaf, William J, LaGory, Edward L, Litzenburger, Ulrike M, Schep, Alicia N, Wei, Yuning   +8 more
core   +2 more sources

Epigenetic Biomarkers for Environmental Exposures and Personalized Breast Cancer Prevention. [PDF]

, 2020
Environmental and lifestyle factors are believed to account for >80% of breast cancers; however, it is not well understood how and when these factors affect risk and which exposed individuals will actually develop the disease.
Park, Hannah Lui
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Environmental assessment of recombinant DNA fermentations

Journal of Industrial Microbiology, 1993
This issue of the Journal of Industrial Microbiology contains a compilation of papers presented at the 1992 National Meeting of the Society for Industrial Microbiology in two symposia entitled 'Environmental Assessment of Recombinant DNA Fermentations'.
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Digging for DNA at depth: rapid universal metabarcoding surveys (RUMS) as a tool to detect coral reef biodiversity across a depth gradient [PDF]

PeerJ, 2019
Background Effective biodiversity monitoring is fundamental in tracking changes in ecosystems as it relates to commercial, recreational, and conservation interests. Current approaches to survey coral reef ecosystems center on the use of indicator species
Joseph D. DiBattista, James D. Reimer, Michael Stat, Giovanni D. Masucci, Piera Biondi, Maarten De Brauwer, Michael Bunce   +6 more
doaj   +2 more sources

Charge-Transfer Matrix Elements by FMO-LCMO Approach: Hole Transfer in DNA with Parameter Tuned Range-Separated DFT [PDF]

, 2015
A scheme for computing charge-transfer matrix elements with the linear combination of fragment molecular orbitals and the 'nonempirically tuned range-separated' density functional is presented.
Ando, Koji, Kitoh-Nishioka, Hirotaka
core   +2 more sources