Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON) [PDF]
Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high
Abraham+23 more
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Electrowetting-Based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay [PDF]
Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the
Coudron, Loic+6 more
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A serological assay to detect SARS-CoV-2 seroconversion in humans [PDF]
Development of an enzyme-linked immunosorbent assay to detect antibodies to the SARS-CoV-2 spike protein in human sera and plasma. Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 ...
Amanat, Fatima+29 more
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Evaluation of efficacy of some serological tests used for diagnosis of brucellosis in cattle in Egypt using latent class analysis [PDF]
In this study serum samples were collected from 4 different groups of cattle, Group I (non-vaccinated Brucella infected group), Group II (Vaccinated Brucella infected group), Group III (Non-vaccinated Brucella free group) and Group IV (vaccinated ...
Abou-Gazia, KA+4 more
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ENZYME LINKED IMMUNOSORBENT ASSAY
The current state of enzyme immunoassay of medicinal substances is considered. The main methods for obtaining and purifying specific antibodies, immunogens, enzyme conjugates are described. Examples of definitions are given medicinal substances by enzyme immunoassay, lists the possible causes of errors in the determination of medicinal substances by ...
Nabieva Farangiz Sadriddinovna,+2 more
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ENZYME LINKED IMMUNOSORBENT ASSAYS REVISITED [PDF]
Enzyme linked immunosorbent assay (ELISA) has now gained wide acclaim in the immunodiagnosis of infectious diseases. ELISA is continuously evolving with newer and sensitive formulations being added to it. The basic tenets of ELISA are discussed. The role of ELISA in two important issues facing us today, namely tuberculosis and human immunodeficiency ...
Mkk Rao, K Kapila, Rajiv Mohan Gupta
openaire +3 more sources
Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA) [PDF]
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay.
Avrameas+17 more
core +1 more source
Enzyme Linked Immunosorbent Assay for Anatid Herpesvirus [PDF]
Duck plague virus causes a hemorrhagic disease affecting the family Anatidae, resulting in 60-80% mortality in captive flocks. Plaque and neutralization assays for Anatid herpesvirus (AHV) require 5-10 days for completion and are restricted by the ...
Lewis, Margaret Anne
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Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease [PDF]
. To date, the sensitivity of the interferon gamma (IFN-c) enzyme-linked immunosorbent assay (ELISA) to detect Johne’s disease (JD) has been poor, especially in the early stages of disease.
Begg, Douglas J.+6 more
core +2 more sources
Gold-black micropillar electrodes for microfluidic ELISA of bone metabolic markers [PDF]
Modification with gold black considerably increased the detection current of protruding micropillar electrodes in microfluidic electrochemical enzyme-linked immunosorbent assay (ELISA)
Fukuda Junji+6 more
core +1 more source