Results 11 to 20 of about 1,860,594 (340)

Inhibition of Tryptophan Hydroxylases and Monoamine Oxidase-A by the Proton Pump Inhibitor, Omeprazole—In Vitro and In Vivo Investigations

Frontiers in Pharmacology, 2020
Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation.
Nibal Betari, Kristoffer Sahlholm, Kristoffer Sahlholm, Kristoffer Sahlholm, Xavier Morató, Xavier Morató, Xavier Morató, Héctor Godoy-Marín, Héctor Godoy-Marín, Olga Jáuregui, Knut Teigen, Francisco Ciruela, Francisco Ciruela, Jan Haavik, Jan Haavik   +14 more
doaj   +1 more source

Novel 1,2,3-Triazole-sulphadiazine-ZnO Hybrids as Potent Antimicrobial Agents against Carbapenem Resistant Bacteria

Antibiotics, 2022
Bacterial pneumonia is considered one of the most virulent diseases with high morbidity and mortality rates, especially in hospitalized patients.
Faizah S. Aljohani, Nadjet Rezki, Mohamed R. Aouad, Mohamed Hagar, Basant A. Bakr, Marwa M. Shaaban, Bassma H. Elwakil   +6 more
doaj   +1 more source

Assaying Paenibacillus alvei CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release

Biomolecules, 2021
Ketalpyruvyltransferases belong to a widespread but little investigated class of enzymes, which utilise phosphoenolpyruvate (PEP) for the pyruvylation of saccharides.
Fiona F. Hager-Mair, Cordula Stefanović, Charlie Lim, Katharina Webhofer, Simon Krauter, Markus Blaukopf, Roland Ludwig, Paul Kosma, Christina Schäffer   +8 more
doaj   +1 more source

Isolation of Peroxisomes from Frozen Liver of Rat by Differential and Iodixanol Gradient Centrifugation

Separations, 2023
In the last decade, research has shown that most diseases are associated with organelle dysfunction in which metabolites play a crucial role or indicate specific processes.
Lina Baz, Mona Al-thepyani, Salha Algarni, Hana Gashlan   +3 more
doaj   +1 more source

Assaying activity and assessing thermostability of hyperthermophilic enzymes [PDF]

, 2001
There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature.
Akanuma, Beynon, Daniel, Daniel, Daniel, Daniel, Danson, Dixon, Fourage, Gerike, Giver, Hensel, Hensel, Hudson, John, Laidler, More, Ramirez, Ratcliffe, Stevens, Tetas, Thomas, Walsh   +22 more
core   +2 more sources

Purification and Crystallographic Analysis of a Novel Cold-Active Esterase (HaEst1) from Halocynthiibacter arcticus

Crystals, 2021
This report deals with the purification, characterization, and a preliminary crystallographic study of a novel cold-active esterase (HaEst1) from Halocynthiibacter arcticus.
Sangeun Jeon, Jisub Hwang, Wanki Yoo, Joo Won Chang, Hackwon Do, Han-Woo Kim, Kyeong Kyu Kim, Jun Hyuck Lee, T. Doohun Kim   +8 more
doaj   +1 more source

Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA) [PDF]

, 1979
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay.
Avrameas, Bennink, Doherty, Doherty, Ertl, Gerhard, Hunter, Koszinowski, Koszinowski, Koszinowski, Leinikki, Lowry, Nakane, Ornstein, Roberts, Salk, Svenag, White   +17 more
core   +1 more source

Development of a Simple Assay Method for Adenosine Deaminase via Enzymatic Formation of an Inosine-Tb³⁺ Complex

Sensors, 2019
Adenosine deaminase (ADA), which catalyzes the irreversible deamination of adenosine to inosine, is related to various human diseases such as tuberculous peritonitis and leukemia.
Suji Lee, Heewon Park, Yeongcheol Ki, Hohjai Lee, Min Su Han   +4 more
doaj   +1 more source

A sensitive homogeneous enzyme assay for euchromatic histone-lysine-N-methyltransferase 2 (G9a) based on terbium-to-quantum dot time-resolved FRET [PDF]

BioImpacts, 2021
Introduction: Histone modifying enzymes include several classes of enzymes that are responsible for various post-translational modifications of histones such as methylation and acetylation. They are important epigenetic factors, which may involve several
Mohammad Amjadi, Tooba Hallaj, Niko Hildebrandt   +2 more
doaj   +1 more source

A method for assaying deubiquitinating enzymes [PDF]

Biological Procedures Online, 1998
A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused alphaNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS,
Kyung Chan Park, Seung Kyoon Woo, Sung Hee Baek, Yung Joon Yoo, Yung Joon Yoo, Keun Il Kim, Jae Il Lee, Chin Ha Chung   +7 more
openaire   +3 more sources