Results 11 to 20 of about 1,218,633 (316)

Neuronal ceroid lipofuscinoses in children

open access: yesAnnals of Indian Academy of Neurology, 2021
Background: The neuronal ceroid lipofuscinoses (NCL) constitute a group of gray matter neurodegenerative disorders characterized by the accumulation of ceroid lipopigment in lysosomes in neurons and other cell types.
Mahesh Kamate   +3 more
doaj   +1 more source

Development of an Enzyme-Based Thin-Layer Chromatographic Assay for the Detection of Cyclooxygenase-2 Inhibitors

open access: yesSeparations, 2022
The search for new anti-inflammatory drugs with less side effects requires simple, fast and reliable screening methods. In this context, we have developed a sensitive thin-layer chromatographic (TLC) assay on silica gel plates to detect cyclooxygenase-2 (
Aurélie Urbain   +2 more
doaj   +1 more source

A sensitive homogeneous enzyme assay for euchromatic histone-lysine-N-methyltransferase 2 (G9a) based on terbium-to-quantum dot time-resolved FRET [PDF]

open access: yesBioImpacts, 2021
Introduction: Histone modifying enzymes include several classes of enzymes that are responsible for various post-translational modifications of histones such as methylation and acetylation. They are important epigenetic factors, which may involve several
Mohammad Amjadi   +2 more
doaj   +1 more source

Conductimetric Enzyme Assays [PDF]

open access: yesEuropean Journal of Biochemistry, 1971
A direct reading, six channel conductivity recording apparatus has been constructed and used to assay enzymes, including some of those found in muscle. This paper gives the essential theory of the method, and indicates that it is a highly versatile assay system. It may be applicable whenever ionic substrates are involved, but this is more easily tested
openaire   +2 more sources

Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)

open access: yesBrazilian Archives of Biology and Technology, 2013
A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E.
Zhen Sun   +3 more
doaj   +1 more source

Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase

open access: yesMolecules, 2022
Hepatitis E virus (HEV) is an understudied pathogen that causes infection through fecal contaminated drinking water and is prominently found in South Asian countries.
Preeti Hooda   +13 more
doaj   +1 more source

Universal buffers for use in biochemistry and biophysical experiments

open access: yesAIMS Biophysics, 2015
The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins.
Dewey Brooke   +2 more
doaj   +1 more source

Insecticidal and Enzyme Inhibition Activities of Leaf/Bark Extracts, Fractions, Seed Oil and Isolated Compounds from Triadica sebifera (L.) Small against Aphis craccivora Koch

open access: yesMolecules, 2022
Aphid, Aphis craccivora Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss.
Shudh Kirti Dolma   +2 more
doaj   +1 more source

Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21 [PDF]

open access: yesIranian Journal of Basic Medical Sciences, 2020
Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and ...
Firoozeh Piroozmand   +3 more
doaj   +1 more source

Simultaneous isolation of mRNA and native protein from minute samples of cells

open access: yesBioTechniques, 2014
Precious biological samples often lack a sufficient number of cells for multiple procedures, such as extraction of mRNA while maintaining protein in a non-denatured state suitable for subsequent characterization.
Tonny Studsgaard Petersen   +1 more
doaj   +1 more source

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