Results 11 to 20 of about 4,652,537 (361)
Journal of Enzyme Inhibition and Medicinal Chemistry, 2020 Metachromatic leukodystrophy (MLD) is a rare genetic disease characterised by a dysfunction of the enzyme arylsulphatase A leading to the lysosomal accumulation of cerebroside sulphate (sulphatide) causing subsequent demyelination in patients. The enzyme
Wenjin Li +6 more
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Biomolecules, 2021 Ketalpyruvyltransferases belong to a widespread but little investigated class of enzymes, which utilise phosphoenolpyruvate (PEP) for the pyruvylation of saccharides.
Fiona F. Hager-Mair +8 more
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Antibiotics, 2022 Bacterial pneumonia is considered one of the most virulent diseases with high morbidity and mortality rates, especially in hospitalized patients.
Faizah S. Aljohani +6 more
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Neuronal ceroid lipofuscinoses in children
Annals of Indian Academy of Neurology, 2021 Background: The neuronal ceroid lipofuscinoses (NCL) constitute a group of gray matter neurodegenerative disorders characterized by the accumulation of ceroid lipopigment in lysosomes in neurons and other cell types.
Mahesh Kamate +3 more
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A sensitive homogeneous enzyme assay for euchromatic histone-lysine-N-methyltransferase 2 (G9a) based on terbium-to-quantum dot time-resolved FRET [PDF]
BioImpacts, 2021 Introduction: Histone modifying enzymes include several classes of enzymes that are responsible for various post-translational modifications of histones such as methylation and acetylation. They are important epigenetic factors, which may involve several
Mohammad Amjadi +2 more
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Separations, 2022 The search for new anti-inflammatory drugs with less side effects requires simple, fast and reliable screening methods. In this context, we have developed a sensitive thin-layer chromatographic (TLC) assay on silica gel plates to detect cyclooxygenase-2 (
Aurélie Urbain +2 more
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Brazilian Archives of Biology and Technology, 2013 A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E.
Zhen Sun +3 more
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Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase
Molecules, 2022 Hepatitis E virus (HEV) is an understudied pathogen that causes infection through fecal contaminated drinking water and is prominently found in South Asian countries.
Preeti Hooda +13 more
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A method for assaying deubiquitinating enzymes [PDF]
Biological Procedures Online, 1998 A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused alphaNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS,
Kyung Chan Park +7 more
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SLAS discovery : advancing life sciences R & D, 2019 Many factors must be considered during the optimization of an enzyme assay. These include the choice of buffer and its composition, the type of enzyme and its concentration, as well as the type of substrate and concentrations, the reaction conditions ...
F. C. Onyeogaziri, C. Papaneophytou
semanticscholar +1 more source