Results 21 to 30 of about 4,652,537 (361)
Universal buffers for use in biochemistry and biophysical experiments
AIMS Biophysics, 2015 The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins.
Dewey Brooke +2 more
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Molecules, 2022 Aphid, Aphis craccivora Koch (Hemiptera: Aphididae), is a major sap-sucking insect pest of leguminous crops and also transmits plant viruses, leading to economic yield loss.
Shudh Kirti Dolma +2 more
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Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21 [PDF]
Iranian Journal of Basic Medical Sciences, 2020 Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and ...
Firoozeh Piroozmand +3 more
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Biochemistry and Molecular Biology Education, 2004 AbstractTerms to be familiar with before you start to solve the test: signal transduction, growth factor signalling, nerve growth factor (NGF), epidermal growth factor (EGF), mitogen‐activated protein kinases (MAPKs), extracellular signal‐regulated kinases (ERKs), MAPK/ERK kinase (MEK), SDS‐PAGE, [γ‐32P]ATP, autoradiography, caspases, protein kinases ...
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Digital enzyme assay using attoliter droplet array
In Analysis, 2018 Attoliter-volume and air-sealed reactor array for fast and robust single-molecule enzyme assay.
T. Ono, T. Ichiki, H. Noji
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Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay [PDF]
Journal of Investigative Dermatology, 2013 inTrOducTiOn Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in various industries; they are also used as analytical tools in biomedical research for the detection and quantification of specific antigens or antibodies in a given sample. These two
Stephanie D. Gan, Kruti R. Patel
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In Analysis, 2015 Digitalization of fluorogenic enzymatic assays through the use of femtoliter chamber array technology is an emerging approach to realizing highly quantitative bioassays with single-molecule sensitivity.
Y. Obayashi, R. Iino, H. Noji
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Simultaneous isolation of mRNA and native protein from minute samples of cells
BioTechniques, 2014 Precious biological samples often lack a sufficient number of cells for multiple procedures, such as extraction of mRNA while maintaining protein in a non-denatured state suitable for subsequent characterization.
Tonny Studsgaard Petersen +1 more
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Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion
mSphere, 2020 In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage.
Michael J. Cohen +2 more
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Metabolites, 2018 The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
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