Results 21 to 30 of about 1,218,633 (316)
Enzymatic Analysis of Yeast Cell Wall-Resident GAPDH and Its Secretion
mSphere, 2020 In yeast, many proteins are found in both the cytoplasmic and extracellular compartments, and consequently it can be difficult to distinguish nonconventional secretion from cellular leakage.
Michael J. Cohen +2 more
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Metabolites, 2018 The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
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Molecules, 2019 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
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A rare PHKA2 variant (p.G991A) identified in a patient with ketotic hypoglycemia
JIMD Reports, 2019 We describe the case of a 4‐year‐old boy who suffered from frequent ketotic hypoglycemia (KH) but did not have hepatomegaly or elevated liver enzyme levels. However, the patient was found to have a rare variant in the PHKA2 gene. To detect the underlying
Yasuhiko Ago +7 more
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Oxygen, 2023 Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling ...
Michal Szczepanczyk +3 more
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A fast, sensitive and fluorescent LPMO activity assay
Frontiers in Microbiology, 2023 Lytic polysaccharide monooxygenases (LPMOs) are industrially relevant enzymes that utilize a copper co-factor and an oxygen species to break down recalcitrant polysaccharides.
Johan Ø. Ipsen +2 more
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Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay [PDF]
Journal of Investigative Dermatology, 2013 inTrOducTiOn Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in various industries; they are also used as analytical tools in biomedical research for the detection and quantification of specific antigens or antibodies in a given sample. These two
Gan, Stephanie D., Patel, Kruti R.
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The Development of Assays for Heparanase Enzymatic Activity: Towards a Gold Standard
Molecules, 2018 The enzyme heparanase, an endo-β-glucuronidase, degrades heparan sulfate (HS) chains on the cell surface and in the extracellular matrix. Heparanase regulates numerous biological processes that drive tumour growth, metastasis and angiogenesis.
Mohit Chhabra, Vito Ferro
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Frontiers in Pharmacology, 2020 Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation.
Nibal Betari +14 more
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BiologyThe present investigation attempts to evaluate the impact of the dietary inclusion of chia (Salvia hispanica) seed oil (CSO) on the indices of haemato-immunology, metabolic enzymes, and expression of immune-responsive cytokine genes in Labeo rohita (rohu)
Sanjay Kumar Gupta +4 more
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