Pharmaceuticals, 2021 Kynurenic acid is a neuroprotective metabolite of tryptophan formed by kynurenine aminotransferase (KAT) catalyzed transformation of kynurenine. However, its high brain levels are associated with cognitive deficit and with the pathophysiology of ...
Michal Maryška +6 more
doaj +1 more source
Synthetic Routes to a Library of Novel Methionine Synthase Inhibitors [PDF]
, 2020 Fungal infections are of continuous concern, especially with regard to immunocompromised patients. In an effort to develop new potential anti-fungal agents, we have begun synthesizing a library of potential inhibitors of the fungal Methionine Synthase ...
Bennett, Zachary +4 more
core +1 more source
Local detection of enzymatic ion generation with polycrystalline silicon interdigitated electrodes and its application to biosensing [PDF]
, 2007 Chips containing polycrystalline silicon interdigitated electrodes are modified with the enzyme urease. The sensors are able to detect changes in the resitivity of the solution near their surface, where the enzymatic reaction generates charged species ...
Antonio Baldi +3 more
core +1 more source
Total tissue lactate dehydrogenase activity in endometrial carcinoma [PDF]
, 2008 Lactate dehydrogenase (LDH) is essential for continuous glycolysis necessary for accelerated tumor growth. The aim of this study was to reconsider if assay of total tissue activity of this enzyme could be useful as marker for endometrial carcinoma (EC ...
Abramić, Marija +4 more
core +1 more source
Molecules, 2019 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
doaj +1 more source
Deactivation of TEM-1 beta-Lactamase investigated by isothermal batch and non-isothermal continuous enzyme membrane reactor methods [PDF]
, 2009 The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR).
Bommarius, Andreas S. +2 more
core +2 more sources
Metabolites, 2018 The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
doaj +1 more source
The Development of Assays for Heparanase Enzymatic Activity: Towards a Gold Standard
Molecules, 2018 The enzyme heparanase, an endo-β-glucuronidase, degrades heparan sulfate (HS) chains on the cell surface and in the extracellular matrix. Heparanase regulates numerous biological processes that drive tumour growth, metastasis and angiogenesis.
Mohit Chhabra, Vito Ferro
doaj +1 more source
A high-throughput mass spectrometric assay for discovery of human lipoxygenase inhibitors and allosteric effectors. [PDF]
, 2015 Lipoxygenases (LOXs) regulate inflammation through the production of a variety of molecules whose specific downstream effects are not entirely understood due to the complexity of the inflammation pathway.
Holman, Theodore R +2 more
core +1 more source
Lipase activity in vesicular systems: Characterization of candida cylindracea lipase and its activity in polymerizable dialkylammonium surfactant vesicles [PDF]
, 1993 Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation.
Engbersen, J.F.J. +2 more
core +4 more sources